Wyniki wyszukiwania

1

Analysis of the murein of a Listeria monocytogenes EGD mutant lacking functional penicillin binding protein 5 [PBP5]

100%

Korsak D., Popowska M., Markiewicz Z.

Polish Journal of Microbiology

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2005

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tom 54

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nr 4

Cells of a mutant of Listeria monocytogenes lacking functional PBP5, an enzyme with DD-carboxypeptidase activity, make thicker cells walls. In this study we show that the muropeptide profile of the mutant, obtained after HPLC analysis of a muramidase digest of cell wall murein, differs from that for the wild type strain. The main differences embrace strongly reduced disaccharide-tripeptide content, strongly increased amounts of pentapeptide-containing muropeptides and a shift in profile from less cross-linked muropeptides (monomers, dimers) towards more highly cross-linked ones.

2

Localization of the attachment site of oligoglucans to Mesorhizobium loti HAMBI 1148 murein

84%

Karas M., Russa R.

Acta Biochimica Polonica

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2009

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tom 56

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nr 1

155-160

The location and nature of the linkage between peptidoglycan and oligoglucans in the cell wall of Mesorhizobium loti HAMBI 1148 have been defined by the analysis of nitrous acid deamination of peptidoglycan glucosaminyl residues. The MurNH2-Glcn fraction was obtained after converting deaminoacylated and N-deacetylated muramyl residues in the cell wall preparation to lactam forms which were stable during subsequent deamination, followed by reduction and opening of the lactams. GC/MS analysis of this material, subjected to partial hydrolysis and reduction or to methanolysis followed by peracetylation, confirmed the presence of glucosyl residues glycosidically attached to muramic acid. The MALDI-TOF spectroscopic analysis of the deaminated material also revealed the presence of [M-H]- or [M+Na-2H]- ions representing fragments containing muramic acid with one to three linked glucose residues. The analysis of fully methylated neutral oligosaccharides released from the peptidoglycan with lysozyme followed by borohydride reduction showed the presence of di- and trisaccharides lacking the reducing end.

3

Effect of benzylpenicillin on murein biosynthesis, turnover and structure in Listeria monocytogenes cells

84%

Siwinska M., Kloszewska M., Loniewska A., Markiewicz Z.

Acta Microbiologica Polonica

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1999

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tom 48

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nr 4

4

Convergence of the beta-lactamase induction and murein recycling pathways in enterobacteria

84%

Szydlowska M., Markiewicz Z.

Acta Microbiologica Polonica

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1997

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tom 46

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nr 3

Ograniczanie wyników

2 Acta Microbiologica Polonica

1 Acta Biochimica Polonica

1 Polish Journal of Microbiology

3 Markiewicz Z.

1 Karas M.

1 Kloszewska M.

1 Korsak D.

1 Loniewska A.

1 Popowska M.

1 Russa R.

1 Siwinska M.

1 Szydlowska M.

1 2009

1 2005

1 1999

1 1997

Analysis of the murein of a Listeria monocytogenes EGD mutant lacking functional penicillin binding protein 5 [PBP5]

Localization of the attachment site of oligoglucans to Mesorhizobium loti HAMBI 1148 murein

Effect of benzylpenicillin on murein biosynthesis, turnover and structure in Listeria monocytogenes cells

Convergence of the beta-lactamase induction and murein recycling pathways in enterobacteria