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Aerodynamic properties of solid materials have long been used to convey and separate seeds and grains duringpost harvest operations. The objective of this study was the evaluation of the aerodynamic properties of mung bean seeds as a function of moisture content and two grades referred to above and below a cut point of 4.8 mm in length. The results showed that as the moisture content increased from 7.8 to 25% (w.b.), the terminal velocity of seeds increased followinga polynomial relationship, from 7.28 to 8.79 and 6.02 to 7.12 m s-1,for grades A and B, respectively. Seeds at grade A had terminal velocities with a mean value of 8.05 m s-1, while at grade B hada mean value of 6.46 m s-1. The Reynolds number of both grades increased linearly with the increase of seeds moisture content, while the drag coefficient decreased with the increase of moisture content. Mathematical relationships were developed to relatethe change in seeds moisture content with the obtained values of aerodynamic properties. The analysis of variance showed that moisture content had a significant effect, at 1% probability level, on all the aerodynamics properties of mung beans.
Seeds of mung bean (Vigma radiata L.) were germinated for 7 days in dark, and the sensory quality of their sprouts was compared using a descriptive sensory analysis and consumer testing. In the descriptive analysis a trained panel (n=9) rated the sprouts for appearance, odour, taste and texture. In the affective tests the panelists rated the sprouts for overall quality. Changes in the contents of total phenolics and tannins were monitored for 7 days of germination using spectrophotometric methods. The results proved that the time of germination had a significant effect on the sensory profiles of the samples. All analysed attributes differentiated statistically significantly the sensory profiles of the sprouts. The overall sensory quality of 3-day-old sprouts was essentially better than that of the other samples. A statistically significant correlation was found between the total phenolics and the overall quality (p=0.05), the total phenolics and bitterness (p=0.01), the total phenolic and astringency (p=0.05), proanthocyanidins and bitterness (p=0.01), astringency and the overall quality (p=0.05), as well as bitterness and the overall quality (p=0.05).
Mung bean CYP90A2 is a putative brassinosteroid (BR) synthetic gene that shares 77% identity with the Arabidopsis CPD gene. It was strongly suppressed by chilling stress. This implies that exogenous treatment with BR could allow the plant to recover from the inhibited growth caused by chilling. In this study, we used proteomics to investigate whether the mung bean epicotyl can be regulated by brassinosteroids under conditions of chilling stress. Mung bean epicotyls whose growth was initially suppressed by chilling partly recovered their ability to elongate after treatment with 24-epibrassinolde; 17 proteins down-regulated by this chilling were re-up-regulated. These up-regulated proteins are involved in methionine assimilation, ATP synthesis, cell wall construction and the stress response. This is consistent with the re-up-regulation of methionine synthase and S-adenosyl-L-methionine synthetase, since chilling-inhibited mung bean epicotyl elongation could be partially recovered by exogenous treatment with DL-methionine. This is the first proteome established for the mung bean species. The regulatory relationship between brassinosteroids and chilling conditions was investigated, and possible mechanisms are discussed herein.
Seeds of local trees, such Azadirachta indica A. Juss, Adenanthera pavonina L., Leucaena leucocephala (Lam.) de Wit and Eucalyptus spp., were used as aqueous extract at 25, 50 and 100 % concentration to control the activity of Meloidogyne javanica (Treub) Citwood. All seed extracts showed lethal effect on M. javanica eggs, and a gradual decrease in egg hatching and an increase in mortality of second-stage juveniles were observed with the increase in extract concentration. L. leucocephala was found to be most effective in reducing egg hatching, whereas 100 % mortality of juveniles was observed in the case of A. indica seed extract. Number of knots was significantly reduced at 100 % concentration when seeds of chick pea and mung bean were treated and soil was drenched with A. pavonina and Eucalyptus spp. seed extract.
The objective of the study was to compare parallel immunoreactive properties and sensory characteristics of lentil (Lens culinaris) and mung bean (Vigma radiata L.) sprouts. The seeds were germinated with and without light at 20°C up to 3 days. The changes in immunoreactivity of the sprouts were determined by competitive ELISA method using rabbit polyclonal antisera to lentil and mung bean proteins. The sensory quality of samples was evaluated by quantitative descriptive analysis (QDA). In QDA a trained panel (n=9) rated the sprouts for odour, taste and texture. The results indicated that the effects of germination on the immunoreactive properties of sprouts with cotyledons depended on the type of legumes. The presence or absence of light during the germination process did not affect the results achieved. The removal of the cotyledons almost completely reduced immunoreactivity of samples (97% for lentil and 99% for mung bean). The effects of germination on the sensory profile of sprouts were found to depend on the type of legumes but as far as the sprouts of mung bean are concerned also on the processing conditions. The QDA demonstrated significant differences between the sprouts for the following attributes: bitterness (p<0.001), “pea-pod” taste (p<0.01), flourness (p<0.001) and juiciness (p<0.01).
The natural resistance against the majority of potential pathogens that exist in most plant species is known as non-host resistance. Several reports suggest the role of antioxidant enzymes in non-host resistance. We assayed the expression or activity of four scavenging enzymes during non-host pathogen-plant interaction (Xanthomonas hortorum pv. pelargonii/mung bean) and host pathogen-plant interaction (Xanthomonas axonopodis pv. phaseoli/mung bean). The expression of superoxide dismutase (SOD) and ascorbate peroxidase (APX) and the enzyme activity of catalase (CAT) and peroxidase (POX) were investigated. The activities of CAT and POX were higher during non-host pathogen invasion vs. host pathogen attack. The expression of SOD and APX were also different between compatible and incompatible interactions. The expression of SOD and APX were higher in the incompatible compared to the compatible interaction. Additionally, induction of the antioxidant enzymes in response to non-host pathogen was earlier than induction in response to host pathogen. Such information is important for plant breeders, and useful when looking for alternative control strategies as well.
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Magnetic-time model at off-season germination

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Effect of static magnetic field on germination of mung beans is described. Seeds of mung beans, were exposed in batches to static magnetic fields of 87 to 226 mT intensity for 100 min. Magnetic time constant – 60.743 Th (Tesla hour) was determined experimentally. High value of magnetic time constant signifies lower effect of magnetic field on germination rate as this germination was carried out at off-season (13°C). Using decay function, germination magnetic constant was calculated. There was a linear increase in germination magnetic constant with increasing intensity of magnetic field. Calculated values of mean germination time, mean germination rate, germination rate coefficient, germination magnetic constant, transition time, water uptake, indicate that the impact of applied static magnetic field improves the germination of mung beans seeds even in off-season.
Each plant species is regarded to substantially influence and thus, select for specific rhizosphere microbial populations. This is considered in the exploitation of soil microbial diversity associated with important crops, which has been of interest in modern agricultural practices for sustainable productivity. This study used PCR-DGGE (polymerase chain reaction - denaturing gradient gel electrophoresis) in order to obtain an initial assessment of the bacterial and fungal communities associated in bulk soil and rhizospheres of different mungbean genotypes under natural field conditions. Integrated use of multivariate analysis and diversity index showed plant growth stage as the primary driver of community shifts in both microbial groups while rhizosphere effect was found to be less discrete in fungal communities. On the other hand, genotype effect was not discerned but not inferred to be absent due to possible lack of manifestations of differences among genotypes based on tolerance to drought under non-stressed environment, and due to detection limits of DGGE. Sequence analysis of prominent members further revealed that Bacillus and Arthrobacter species were dominant in bacterial communities whereas members of Ascomycota and Basidiomycota were common in fungal communities of mungbean. Overall, fungal communities had higher estimated diversity and composition heterogeneity, and were more dynamic under plant growth influence, rhizosphere effect and natural environmental conditions during mungbean growth in upland field. These primary evaluations are prerequisite to understanding the interactions between plant and rhizosphere microorganisms with the intention of employing their potential use for sustainable crop production.
Drought is one of the major abiotic stresses which adversely affect crop growth and production worldwide as water is vital for every aspect of plant growth and development. The present experiment was carried out during the growing seasons (September – December) of 2012 and 2013 to evaluate the response of black gram (Vigna mungo L.) and green gram (Vigna radiata L.) in terms of some important growth indices, biochemical traits and seed quality under drought stress. Four commonly grown genotypes - T9, KU 301(black gram) and Pratap, SG 21-5 (green gram) of Assam, India were grown in a randomized block design with three replications under stress and non-stress conditions. Stress was applied by withholding irrigation for fifteen consecutive days at vegetative, flowering and pod filling stages. Leaf area index (LAI), seed protein content and protein yield significantly decreased (p ≤ 0.01) whereas proline, total flavonoids and anthocyanin content increased significantly (p ≤ 0.01) in response to water deficiency. Among the studied genotypes, T9 and Pratap showed better tolerance capacity towards the applied drought by presenting higher values of LAI, plant height stress tolerance index (PHSI), dry matter stress tolerance index (DMSI), proline, total flavonoids, anthocyanin, lower percentage of chlorophyll degradation and finally producing high quality seeds.
A experiment was conducted to investigate the efficacy of different plant derivatives against the development of the green gram pest Callosobruchus chinensis (L). fed on green gram seeds Vigna radiate for one month period. The leaf extracts of lemon (Citrus lemon), mint (Mentha arvensis) and black pepper seed (Piper nigram) powders were evaluated for their growth, adult mortality and oviposition inhibition of Callosobruhus chinensis. The results revealed that the extracts of all the three plant products caused a considerable reduction in the number of bruchids. Among the treatments Piper nigram were found to be effective in destroying the pest.
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