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Micromorphological characterisation and the comparative statistical analysis of the size of Primula veris L. pollen grains collected in three natural and three cultivated populations were done. Observations were carried out with SEM. The obtained measurements were analysed with the use of one-way ANOVA, Kruskal-Wallis Test and the Student-t Test. Pollen grains from long-styled ('pin') flower-morphs were mainly 6 colpate and from shortstyled ('thrum') flower-morphs 8 colpate. Colpi of some grains from 'thrum' flowers were 'sinuous' and 'circular', and they incised into the apocolpium zone. Ornamentation of 'pin' pollen grains was microreticulate, with lumina up to 0.8 μm wide, and for pollen grains from 'thrum' flowers was reticulate and eureticulate with lumina 1.1-1.7 μm wide. In lumina of mesocolpium area some free columellae were observed. Pollen grains from 'thrum' flower-morphs were more variable in size, both in natural and in cultivated populations, than grains from 'pin' flower-morphs. The differences in mean length (P) and breadth (E) of pollen grains from 'thrum' flowers collected in cultivated populations were statistically important (FP = 3.154 for the critical F005 = 3.098; K-We = 7.469 for the critical Test value α=005 = 5.991). Pollen grains from 'thrum' flowers were bigger when coming from plants growing in natural populations (tE = 2.784 for the critical Test value α=005 = 2.001).
Faunistic, distributional, and taxonomic data for the genus Phalangium Linnaeus, 1758 from the Caucasus are presented. Eight species of the genus have been found in the region. Two new species: P. mcheidzeae from Georgia and P. gorbunovi from Volgograd region, Kalmikiya and Krasnodar region are described, the other six species of the area are P. opilio, P. punctipes, P. armatum, P. staregai, P. bakuense and P. armenicum. Each of them is illustrated, their main diagnostic features are summarized and the occurrence of each species is presented. A key to the species of Phalangium of the region is provided.
During two years of the investigations some morphological properties as well as the chemical composition of the one cultivar of the meadow clover (Trifolium pretense) and white clover (Trifolium repens) were compared with that of their ecotypes. The meadow clover was charac­terized in its first regrowth in cutting maturity and the white clover in the second regrowth in its pasture maturity. In both cultivars examined properties were characterized with the statistically higher parameters than in their ecotypes. The greatest differences between cultivars and ecotypes did not refer to the chemical composition.
Taxonomy of the genus Quercus L. is very complicated and often controversial because of its great variability and intense gene flow among the related species. The purpose of this research was to determine morphological and molecular variation, relationships and taxonomic status of the Croatian populations of Quercus pubescens Willd. using morphological analysis of the leaves and RAPD-PCR technique. The results of the morphological and molecular analyses were very similar, both showing differentiation of the southern (Mediterranean) from the northern (Continental) pubescent oak populations. These two groups were clearly separated and the estimated gene flow among the populations that belong to different groups (Nm=1.38) is significantly less than among the populations that belong to the same group (Nm=3.70). The obtained results were compared to the available studies. This study confirms a high variability of the Q. pubescens populations, but differences were not so big to confirm the opinion of existence of several species in this area. The conclusion is that the southern Croatian populations could be pure Q. pubescens populations, while the peculiarities of the northern Croatian populations originate probably from the Q. petraea introgression.
The aim of the present study was biometrical comparison of three Iberian populations of Juniperus phoenicea, represented by the subsp. turbinata and subsp. phoenicea. Eight features of the cones and seeds, two of the shoots and leaves were studied. The biometrical analysis of three distant populations of J. phoenicea shows great taxonomic distances among them. Two of them, representatives of J. phoenicea subsp. turbinata, are closer related each other than to the third, which represents J. phoenicea subsp. phoenicea. These results confirm the genetic differentiation of the taxons and also the biochemical and morphologic division of them. Nevertheless, the distances between particular populations are so great that more resemble the distances between species than between subspecies.
Males, females, and larvae of Carios fonsecai sp. nov. are described from free-living ticks collected in a cave at Bonito, state of Mato Grosso do Sul, Brazil. The presence of cheeks and legs with micromammillate cuticle makes adults of C. fonsecai morphologically related to a group of argasid species (mostly bat-associated) formerly classified into the subgenus Alectorobius, genus Ornithodoros. Examination of larvae indicates that C. fonsecai is clearly distinct from most of the previously described Carios species formerly classified into the subgenus Alectorobius, based primarily on its larger body size, dorsal setae number, dorsal plate shape, and hypostomal morphology. On the other hand, the larva of C. fonsecai is most similar to Carios peropteryx, and Carios peruvianus, from which differences in dorsal plate length and width, tarsal setae, and hypostome characteristics are useful for morphological differentiation. The mitochondrial 16S rDNA sequence of C. fonsecai showed to be closest (85–88% identity) to several corresponding sequences of different Carios species available in GenBank. Bats identified as Peropteryx macrotis and Desmodus rotundus were found infested by C. fonsecai larvae in the same cave where the type series was collected. C. fonsecai showed to be aggressive to humans in the laboratory.
A good knowledge of species delimitation is crucial for the biodiversity protection and the conservation of wild species. We studied the efficiency of AFLP markers and morphological characters to assist species determination for Melica ciliata L. and M. transsilvanica Schur within European range of distribution, including isolated and range-limit populations of "M. ciliata" (i.e. M. cf. ciliata) from the Polish Sudetes, where it is regarded as critically endangered. AFLP markers were found to be more effective then morphological characters (more or less continuous) in distinguishing the both studied species. AMOVA revealed very low genetic diversity within populations and high differentiation among populations of M. ciliata and M. transsilvanica (FST = 0.89 and 0.95, respectively). The species-diagnostic AFLP markers of M. transsilvanica shared with "M. ciliata" from the Sudetes were detected. On the other hand, no species-diagnostic genetic markers of M. ciliata or hybrid-diagnostic markers of M. × thuringiaca were found within "M. ciliata". PCoA and NJ showed an overlapping genetic diversity of "M. ciliata" and M. transsilvanica. Hierar­chical AMOVA supported the absence of a significant genotypic distinction between "M. ciliata" and M. transsilvanica. ANOVA showed that the length ratio of lower to upper glumes was the best morphological character to discriminate between M. ciliata and M. transsilvanica. Combined morphological and genetic data show that M. ciliata is not currently present in Poland as its putative Polish populations represent M. transsilvanica. A significant decrease in genetic varia­bility that could influence viability was not observed the in Sudetian populations of M. transsilvanica. However, the population size changes significantly as a result of plant succession. Correction of the northern limit of the continuous distribution of M. ciliata L. in Central Europe is presented.
The structure, micromorphology and distribution of trichomes on Dracocephalum moldavicum L. shots were investigated using light and scanning electron microscopy (SEM). There were distinguished 3 types of non-glandular trichomes, 3 types of glandular trichomes and papillae of the osmophore in the epidermis of the corolla. The highest density of non-glandular and glandular trichomes was found on the abaxial surface of the calyx, on bracts and in the upper part of the stem. Structural variations in the head and stalk of long glandular trichomes were demonstrated. The dimensions of particular types of trichomes are given in the present paper. Differences in the structure of the trichomes of this species are presented, compared to literature data. The micromorphology of particular trichome types is documented in numerous photographs showing the rich ornamentation of the cuticle of non-glandular trichomes.
Pinus eldarica has been introduced to Iran from more than 800 years ago. Some individuals of this pine have altered both in shape and growth rate in northeastern of Iran, and generated two distinct morphotypes which are called Conical-shaped and Ball-shaped pines. This study was conducted to discriminate these morphotypes using macromorphological and anatomical characteristics. Results of macromorphological analysis showed significant differences both with univariate and multivariate analysis and consequently two new morphotypes were clearly separated from Mondell pine individuals. Furthermore, anatomical differences observed in Conical-shaped pine in comparison with two other pines, from some valuable taxonomical point of view traits such as cross-section form of needle, number of stomata per area, number and position of resin ducts etc. Furthermore, the existent difference in traits like cuticle thickness, stomata density, needle perimeter and length, state increasing the adaptation potential to aridity in Conical-shaped pine in comparison with two others. The differences of two new morphotypes demonstrate that they are new variants of Mondell pine and it is need to be used molecular markers and phylogenic studies for specifying the cause of these morphological and anatomical differences.
Badano zależności między morfologią kwiatów marchwi męskosterylnych i męskopłodnych linii hodowlanych i zdolnością do wytwarzania nasion. W badanej populacji męskosterylnych roślin marchwi kwiaty charakteryzowały się przekształceniem pręcików w dodatkowe struktury płatkopodobne o różnych kształtach. W niektórych pylnikopodobnych strukturach kwiatu stwierdzono obecność degenerującej tkanki sporogennej. Wyniki obserwacji i analiz mikroskopowych pozwalają twierdzić, że na obniżenie produktywności nasion linii hodowlanych marchwi mogły mieć wpływ defekty i nieprawidłowości morfologiczno-funkcjonalne kwiatów roślin ojcowskich oraz matecznych. Degeneracja pyłku, brak warstwy endotecjum, decydującej o otwieraniu się pylnika, wzrost łagiewek pyłkowych po zewnętrznej powierzchni szyjki słupka należały do najczęściej występujących ograniczeń w ilości pyłku aktywnego w zapłodnieniu. Cechy męskosterylnych roślin matecznych utrudniające proces zawiązywaniu nasion to: zielona barwa płatków, redukcja miodników, deformacje słupków, brak zalążni, degeneracja zalążków lub zarodków. Najmniej nasion wytworzyły rośliny z licznymi deformacjami morfologicznymi znamion słupka i miodników. Kwiaty roślin męskosterylnych o białych płatkach, dużych miodnikach i wysokich, podwójnych słupkach wydały najwięcej nasion, gdyż były prawdopodobnie najbardziej atrakcyjne dla zapylających owadów.
The aim of the present study was to determine the relation between the quality of seed germination and morphological differentiation of seedlings of the studied tomato cultivars. The seeds of very early tomato cultivars (Beta, Betalux, Promyk) compared to the semi-early New Yorker cultivar treated as control were subjected to evaluation. It was found out that their germination rate significantly affected the quantity of obtained seedlings. The seedlings grown from seeds that germinated after 5 days were significantly higher than those that germinated after 7 or 10 days. They also had higher number of leaves, larger diameter of root collar and higher fresh mass.
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