Wyniki wyszukiwania

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Detection of myxoma virus in the classical form of myxomatosis using an AGID assay: statistical assessment of the assay’s diagnostic performance

100%

Kwit E., Osinski Z., Lavazza A., Rzezutka A.

Journal of Veterinary Research

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2020

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tom 64

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nr 3

2

RAPD analysis and numerical taxonomy methods in identification of wheat cultivars

100%

Borsuk P., Batko A., Czaplicki A. Z.

Journal of Applied Genetics

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1996

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tom 37A

3

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The importance of molecular identification methods of mosquitoes in epidemiology of vector-borne diseases

86%

Rydzanicz K., Lonc E., Becker N.

Annals of Parasitology

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2016

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tom 62

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nr Suppl.

4

Advanced methods of bacteriological identification in a clinical microbiology laboratory

86%

Zukowska M. E.

Journal of Pre-Clinical and Clinical Research

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2021

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tom 15

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nr 2

5

An improved procedure of the metagenomic DNA extraction from saline soil, sediment and salt

86%

Kashi F. J.

International Letters of Natural Sciences

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2016

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tom 60

6

Molecular methods for rapid detection of aneuploidy

86%

Dudarewicz L., Holzgreve W., Jeziorowska A., Jakubowski L., Zimmermann B.

Journal of Applied Genetics

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2005

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tom 46

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nr 2

Rapid molecular biological methods for prenatal diagnosis of the most common aneuploidies, collectively known as rapid aneuploidy testing, are compared in this review. We discuss methodological problems and limitations of these various methods. All these techniques are believed to be accurate and carry a low risk of misdiagnosis, but they differ in terms of labour-intensity and amenability to automation and high throughput testing. The question how to apply them safely and economically in a clinical setting has not been answered yet. The discussed techniques are so far not used as stand-alone tests, but some of them are routinely applied as a preliminary test that shortens the waiting time for classic cytogenetic karyotyping. In the future, mainly because of economical reasons, these methods may replace cytogenetics in the category of patients who make up the majority of those currently offered prenatal karyotyping: patients with moderately increased risk and no abnormalities detected by ultrasound.

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The modern classification of cyanoprokaryotes [Cyanobacteria]

86%

Komarek J.

Oceanological and Hydrobiological Studies

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2005

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tom 34

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nr Suppl.3

8

Progress in the molecular methods for the detection and genetic characterization of Cryptosporidium in water samples

72%

Skotarczak B.

Annals of Agricultural and Environmental Medicine

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2010

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tom 17

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nr 1

1-8

9

PCR and real time PCR for the detection of Cryptosporidium parvum oocyst DNA

72%

Adamska M., Leonska-Duniec A., Maciejewska A., Sawczuk M., Skotarczyk B.

Folia Biologica

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2011

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tom 59

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nr 3-4

10

Detection of resistance to anti-tuberculosis drugs in the clinical isolates of Mycobacterium tuberculosis from Slovakia through comparison between phenotypic and genetic methods and evaluation of resistance levels with clinical parameters

72%

Mokry J., Porvaznik I., Kusnir P., Dohal M., Solovic I.

Journal of Physiology and Pharmacology

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2019

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tom 70

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nr 1

11

Intra-specific differentiation of Paramecium bursaria strains by molecular methods - preliminary studies

72%

Greczek-Stachura M., Tarcz S., Przybos E.

Folia Biologica

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2010

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tom 58

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nr 1-2

35-45

12

Use and comparison of molecular and classical methods for the identification of moulds isolated from the soil

72%

Piegza M., Rzasa J., Siepka E., Witkowska D.

Acta Scientiarum Polonorum. Biotechnologia

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2010

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tom 09

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nr 4

Present work contains the attempt to compare methods of microorganisms identification, for example, the similarity between species – molecular and classical. The both methods were used because classical approaches based on the use of morphological criteria are, as in several other fungi, difficult to apply in Trichoderma, due to plasticity of characters to interpret. Consequently, almost all recent studies have used molecular data to characterize and identify species. The comparison of these two techniques is based on using guidelines of macroscopic and microscopic evaluation of few classical factors as well as RAPD and RFLP – molecular techniques. Results indicate that none of the technique being compared is adequate when used alone, although molecular ones are more accurate. There is no complete convergence between RAPD and RFLP what makes it even harder to interpret but this fact can be the result of inconvenient markers used in experiment.

13

Molecular methods as ways of dealing with terminological difficulties in fungal ecology

72%

Budziszewska J., Piatkowska J.

Ecological Questions

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2009

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tom 11

Many ecological terms are very difficult to define, or to apply to some groups of organisms. In the case of fungi, it is unclear how to speak of individuals, populations and species. Relying on morphological characters often gives ambiguous results. However, in recent years new techniques have been developed, techniques that provide us with robust tools, helpful in recognizing specific groups in nature. This paper aims to give a short overview of how molecular methods are used to delimitate species, individuals and populations in fungi.

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Different DNA methylation pattern in A and B chromosomes of Crepis capillaris detected by in situ nick-translation. Comparison with molecular methods

72%

Luchniak P., Maluszynska J., Olszewska M. J.

Folia Histochemica et Cytobiologica

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2002

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tom 40

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nr 3

15

Application of molecular methods for typing of H.pylori for monitoring of treatment and epidemiological studies

72%

Dzierzanowska D., Gzyl A.

Journal of Physiology and Pharmacology. Supplement

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1997

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tom 48

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nr 1

16

Identification of Lactobacillus strains present in fermented dairy products and their differentiation using molecular methods

72%

Markiewicz L. H., Wasilewska E., Bielecka M.

Polish Journal of Food and Nutrition Sciences

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2008

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tom 58

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nr 2

The aim of the study was to identify and differentiate five strains isolated from fermented dairy products using species-specific polymerase chain reaction (PCR) as well as PCR of internal transcribed spacer (ITS-PCR) and pulsed field gel electrophoresis (PFGE) in reference to type strains. Results of species-specific PCRs showed that three strains belonged to the species of L. helveticus and two strains to the species of L. casei. Results obtained with both IST-PCR and PFGE method showed low diversity of the isolates since only three different ITS-PCR and PFGE profiles were obtained. Moreover, differentiation conducted merely with the PFGE method allowed distinguishing the type L. casei DSMZ20011, L. rhamnosus DSMZ20021 and L. paracasei subsp. paracasei DSMZ5622 strains. Results of this study confirmed that, although time-consuming and expensive, the PFGE method was characterised by the highest discriminatory power in strain differentiation. The ITS-PCR method even though fast, easy and relatively inexpensive, showed to be more suitable for the pre-selection of strains.

17

Life cycle, morphology, ontogenesis, and phylogeny of Bromeliothrix metopoides nov. gen., nov. spec., a peculiar ciliate [Protista, Colpodea] from tank bromeliads [Bromeliaceae]

58%

Foissner W.

Acta Protozoologica

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2010

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tom 49

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nr 3

159-193

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Zastosowanie metod molekularnych do wykrywania wirusa lisciozwoju ziemniaka [PLRV]

58%

Treder K., Lewosz J.

Progress in Plant Protection

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2000

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tom 40

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nr 1

177-187

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Wykrywanie bakterii Salmonella metodami molekularnymi w produktach żywnościowych

58%

Misiewicz A., Goncerzewicz A.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2013

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tom 573

Bakterie z rodzaju Salmonella są najbardziej znanymi bakteriami patogennymi występującymi w przemyśle spożywczym. Powodują zakażenia prawie wszystkich produktów żywnościowych – od mięsnych, mlecznych, jajecznych po rośliny oleiste i pasze. Wykrycie i identyfi kacja bakterii Salmonella na podstawie tradycyjnej metody mikrobiologicznej, zgodnie z normą PN-EN 6579:2003, jest ciągle powszechnie stosowane w laboratoriach. Analiza ta jest czaso- i pracochłonna, jej wykonanie trwa około 5–7 dni. Wykorzystanie nowoczesnych technik biologii molekularnej, z etapem namnożenia i izolacji DNA, do uzyskania końcowego wyniku trwa znacznie krócej. W niniejszej pracy zastosowano metodę Real-Time PCR i klasyczny PCR do identyfi kacji bakterii Salmonella w różnych produktach żywnościowych. Określono czas potrzebny do wykonania tej analizy molekularnej. Do reakcji Real-Time PCR wykorzystano komercyjny kit. Klasyczną reakcję PCR prowadzono z użyciem starterów Sal465Li Sal142F, uzyskując właściwy produkt o wielkości 343 pz. We wszystkich badanych próbkach wykryto bakterie Salmonella. Wynik analiz molekularnych uzyskano w ciągu 21–25 godzin.

20

Usefulness of PCR/RFLP and ERIC PCR techniques for epidemiological study of Haemophilus parasuis infections in pigs

58%

Jablonski A., Zebek S., Kolacz R., Pejsak Z.

Polish Journal of Veterinary Sciences

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2011

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tom 14

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nr 1

Haemophilus parasuis belongs to opportunistic microorganisms of undefined virulence. The purpose of the studies was to compare suitability of PCR/RFLP in our modification and ERIC PCR for epidemiological study of domestic strains of H. parasuis. The results were evaluated taking into account two different aspects: suitability of the tests for isolating the highest possible number of clone groups and subjective evaluation of the method judged with respect to the following criteria: difficulty, availability of equipment and reagents as well as time and cost of the study. The results obtained in the present study show that the two methods used for typing of H. parasuis had high discriminatory power. Taking into account this parameter it can be concluded that ERIC PCR is more suitable than PCR/RFLP. This justifies the use of ERIC PCR for routine epidemiological analyses of mentioned pathogen. Taking into account the complexity of method used, ERIC-PCR based on random amplification of DNA, proved to be comparable to PCR/RFLP. The last mentioned technique is relatively less expensive and labour-consuming, especially when diagnostic PCR method is used for the epidemiological studies.

Ograniczanie wyników

Detection of myxoma virus in the classical form of myxomatosis using an AGID assay: statistical assessment of the assay’s diagnostic performance