Wyniki wyszukiwania

1

Bacterial diversity in the rumen of Indian Surti buffalo (Bubalus bubalis), assessed by 16S rDNA analysis

100%

Pandya P. R., Singh K. M., Parnerkar S., Tripathi A. K., Mehta H. H., Rank D. N., Kothari R. K., Joshi C. G.

Journal of Applied Genetics

|

2010

|

tom 51

|

nr 3

Bacterial communities in buffalo rumen were characterized using a culture-independent approach for a pooled sample of rumen fluid from 3 adult Surti buffaloes. Buffalo rumen is likely to include species of various bacterial phyla, so 16S rDNA sequences were amplified and cloned from the sample. A total of 191 clones were sequenced and similarities to known 16S rDNA sequences were examined. About 62.82% sequences (120 clones) had >90% similarity to the 16S rDNA database sequences. Furthermore, about 34.03% of the sequences (65 clones) were 85-89% similar to 16S rDNA database sequences. For the remaining 3.14%, the similarity was lower than 85%. Phylogenetic analyses were also used to infer the makeup of bacterial communities in the rumen of Surti buffalo. As a result, we distinguished 42 operational taxonomie units (OTUs) based on unique 16S r DNA sequences: 19 OTUs affiliated to an unidentified group (45.23% of total OTUs), 11 OTUs of the phylum Firmicutes, also known as the low G+C group (26.19%), 7 OTUs of the Cytophaga-Flexibacter-Bacteroides phylum (16.66%), 4 OTUs of Spirochaetes (9.52%), and 1 OTU of Actmobacteria (2.38%). These include 10 single-clone OTUs, so Good's coverage (94.76%) of 16S rRNA libraries indicated that sequences identified in the libraries represent the majority of bacterial diversity present in rumen.

2

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Molecular diversity of two-spotted spider mite (Tetranychus urticae) in apple orchards across Poland and its potential link with the resistance to METI-acaricides

84%

Jecz T., Korbin M., Gorzka D., Mohamed A.-R. A., Olszak R.

Journal of Horticultural Research

|

2018

|

tom 26

|

nr 2

3

Assessment of molecular diversity in chickpea (Cicer arietinum L.) rhizobia and structural analysis of 16S rDNA sequences from Mesorhizobium ciceri

84%

Yadav A., Singh A. L., Rai G. K., Singh M.

Polish Journal of Microbiology

|

2013

|

tom 62

|

nr 3

Molecular diversity studies of 19 rhizobia isolates from chickpea were conducted using simple sequence repeats (SSR) and 16S rDNA-RFLP markers. Phenotypic characterization with special reference to salinity and pH tolerance was performed. These isolates were identified as different strains of Mesorhizobium, Rhizobium, Bradyrhizobium, and Agrobacterium. Twenty SSR loci of Mesorhizobium ciceri, distributed across the other rhizobial genome, clearly differentiated 19 rhizobial isolates. Analogous clustering supported the results of 16S rDNA sequence-based phylogeny. Analysis of the 16S rDNA sequences from M. ciceri strains revealed that nucleotide variables (signature sites) were located at 20 different positions; most of them were present in the first 820 bp region from 5’ terminal. Interestingly, 14 signature sites were located in two main regions, the variable region V1 (nt 527–584), and variable region V2 (nt 754–813). The secondary structure and minimal free energy were determined in these two regions. These results will be useful in characterizing the micro-evolutionary mechanisms of species formation and increase understanding of the symbiotic relationship.

4

Use of DNA markers for assessing the genetic identity between native and introduced stone pine [Pinus cembra] in the Tatra Mountains

84%

Chmiel J., Polok K.

Seria Biologiczna. Uniwersytet im. Adama Mickiewicza w Poznaniu

|

2005

|

tom 72

5

Evolutionary divergence within Pellia endiviifolia [Dicks.] Dum. from Poland

84%

Polok K., Sawicki J., Kubiak K., Szczecinska M., Korzekwa K., Szandar K., Zielinski R.

Seria Biologiczna. Uniwersytet im. Adama Mickiewicza w Poznaniu

|

2005

|

tom 72

6

The molecular diversity of different isolates of Beauveria bassiana [Bals.] Vuill. as assessed using inter-microsatellites [ISSRs]

84%

Estrada M. E., Camacho M. V., Benito C.

Cellular and Molecular Biology Letters

|

2007

|

tom 12

|

nr 2

Inter-microsatellite PCR (ISSR-PCR) markers were used to identify and to examine the genetic diversity of eleven Beauveria bassiana isolates with different geographic origins. The variability and the phylogenetic relationships between the eleven strains were analyzed using 172 ISSR-PCR markers. A high level of polymorphism (near 80%) was found using these molecular markers. Seven different isolates showed exclusive bands, and ISSR primer 873 was able to distinguish between all the strains. The dendrogram obtained with these markers is robust and in agreement with the geographical origins of the strains. All the isolates from the Caribbean region were grouped together in a cluster, while the other isolates grouped in the other cluster. The similarity exhibited between the two clusters was less than 50%. This value of homology shows the high genetic variability detected between the isolates from the Caribbean region and the other isolates. ISSR-PCR markers provide a quick, reliable and highly informative system for DNA fingerprinting, and allowed the identification of the different B. bassiana isolates studied.

Ograniczanie wyników

Bacterial diversity in the rumen of Indian Surti buffalo (Bubalus bubalis), assessed by 16S rDNA analysis

Molecular diversity of two-spotted spider mite (Tetranychus urticae) in apple orchards across Poland and its potential link with the resistance to METI-acaricides

Assessment of molecular diversity in chickpea (Cicer arietinum L.) rhizobia and structural analysis of 16S rDNA sequences from Mesorhizobium ciceri

Use of DNA markers for assessing the genetic identity between native and introduced stone pine [Pinus cembra] in the Tatra Mountains

Evolutionary divergence within Pellia endiviifolia [Dicks.] Dum. from Poland

The molecular diversity of different isolates of Beauveria bassiana [Bals.] Vuill. as assessed using inter-microsatellites [ISSRs]