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Recent studies suggest that horizontal gene transfer (HGT) played a significant role in the evolution of eukaryotic lineages. We here review the mechanisms of HGT in plants and the importance of HGT in land plant evolution. In particular, we discuss the role of HGT in plant colonization of land, phototropic response, C4 photosynthesis, and mitochondrial genome evolution.
A survey of fully-sequenced chloroplast genomes revealed that in land plants there are six tRNA genes that have introns. Moreover, the length of a particular tRNA gene intron remains relatively stable across species. However, in algae, the presence of chloroplast tRNA genes containing introns is exceptional. A survey of mitochondrial plant genomes revealed intron-containing tRNA genes are rather rare features, with the exception of tRNASerGCU genes in liverworts and peat-mosses. We isolated and sequenced one mitochondrial and three chloroplast intron-containing tRNA genes and a fragment of the mitochondrial coxIII gene containing the first intron from the following liverwort species: Pellia borealis, Pellia epiphylla-species N, Pellia epiphylla-species S and Porella baueri, Porella cordaeana, Porella platyphylla. We showed that, as in the case of higher plants, the rate of nucleotide substitution is lower in the mitochondrial genome than in the chloroplast genome. Moreover, the comparison of intron nucleotide sequences enabled us to show that in the case of one allopolyploid species, Pellia borealis, organelles were transmitted from one parent species, Pellia epiphylla-species N. In the case of another allopolyploid species, Porella baueri, organelles were also inherited from one parent species, Porella cordaeana. Therefore, organellar inheritance in liverworts seems to be uniparental. It remains clear that analysis of carefully chosen chloroplast and mitochondrial DNA sequences allowed us to reconstruct historical events.
We present the effects of cytoplasm substitution on five productivity traits in an alloplasmic barley collection. 60 lines combining 5 nuclear genomes of cultivated barley varieties and 12 plasmons of two barley species (H. vulgare, H. spontaneum) displayed various effects depending on definite nuclei-cytoplasm combinations. Only four cytoplasmic genomes (W1, W4, W5, W10) significantly modified the expression of the nuclear genes controlling productivity. RAPD-PCR analysis revealed that both the mitochondrial and chloroplast DNA of the W1, W5, and W10 lines have common molecular characters distinguishing them from the cytoplasmic genomes of the other lines. The cytoplasmic genetic factors influencing the expression of "productivity" genes remain elusive.
Mitochondrial genomes are frequently used to infer phylogenetic relationships. Some taxa are, however, poorly represented. To facilitate better understanding of the potential of mitochondrial genome data in freshwater mussels, we present here, for the first time, the mitochondrial sequences of 4 complete F-type mitochondrial genomes from the European freshwater bivalve Unio pictorum (Unionidae). These genomes are very compact (15 761 bp) but have atypical gene complement for bilaterian mitochondrial genomes and a very similar organization to other unionid genomes available in databases. Very low nucleotide diversity within the species suggests a small effective population size of Polish U. pictorum, a phenomenon of potential importance for environmental management policies.
This review focuses on plant-to-plant horizontal gene transfer (HGT) involving the three DNA-containing cellular compartments. It highlights the great incidence of HGT in the mitochondrial genome (mtDNA) of angiosperms, the increasing number of examples in plant nuclear genomes, and the lack of any convincing evidence for HGT in the well-studied plastid genome of land plants. Most of the foreign mitochondrial genes are non-functional, generally found as pseudogenes in the recipient plant mtDNA that maintains its functional native genes. The few exceptions involve chimeric HGT, in which foreign and native copies recombine leading to a functional and single copy of the gene. Maintenance of foreign genes in plant mitochondria is probably the result of genetic drift, but a possible evolutionary advantage may be conferred through the generation of genetic diversity by gene conversion between native and foreign copies. Conversely, a few cases of nuclear HGT in plants involve functional transfers of novel genes that resulted in adaptive evolution. Direct cell-to-cell contact between plants (e.g. host-parasite relationships or natural grafting) facilitate the exchange of genetic material, in which HGT has been reported for both nuclear and mitochondrial genomes, and in the form of genomic DNA, instead of RNA. A thorough review of the literature indicates that HGT in mitochondrial and nuclear genomes of angiosperms is much more frequent than previously expected and that the evolutionary impact and mechanisms underlying plant-to-plant HGT remain to be uncovered.
Southern blots of mitochondrial (mt) DNAs of three Lupinus species cleaved with three restriction enzymes were probed with Lupinus luteus mtDNA fragments containing 18S, 5S rRNA genes or a tRNA-like repeat. Comparison of the number of hybridizing bands and their intensity suggested that the mt 18S and 5S rRNA genes occur mostly in one copy in the genomes of three lupin species. The exception concerned the Lupinus angustifolius 5S rRNA gene showing two hybridizing bands of unequal intensity. The results of hybridization of the lupin mitochondrial genomes with a probe specific for the Lupinus luteus tRNA-like repeat pointed to the presence of such a repeat in other parts of the genomes besides the vicinity of the 18S rRNA gene. Northern hybridization analysis showed the presence of 18S, 5S and tRNA-like repeat transcripts similar in size in all lupin species.
Genetic information of human is encoded in two genomes: nuclear and mitochon­drial. Both of them reflect molecular evolution of human starting from the beginning of life (about 4.5 billion years ago) until the origin of Homo sapiens species about 100000 years ago. From this reason human genome contains some features that are common for different groups of organisms and some features that are unique for Homo sapiens. 3.2 X 10 base pairs of human nuclear genome are packed into 23 chromosomes of different size. The smallest chromosome - 21st contains 5 X 10 7 base pairs while the biggest one -1st contains 2.63 X 10 8 base pairs. Despite the fact that the nucleotide sequence of all chromosomes is established, the organisation of nuclear genome put still questions: for example: the exact number of genes encoded by the human genome is still unknown giving estimations from 30 to 150 thousand genes. Coding sequences represent a few percent of human nuclear genome. The ma­jority of the genome is represented by repetitive sequences (about 50%) and noncoding unique sequences. This part of the genome is frequently wrongly called "junk DNA". The distribution of genes on chromosomes is irregular, DNA fragments containing low percentage of GC pairs code lower number of genes than the frag­ments of high percentage of GC pairs.
Background. A new taxonomy of tubenose gobies of the genus Proterorhinus has been proposed recently. However, some of taxonomic hypotheses have not been confirmed by recent genetic studies or seem not to correspond with ecological features and genetic differentiation of these fishes. The aim of this paper was to test the new taxonomy through a phylogeographic analysis of the fish from 15 sites in Southern Ukraine and Russia, through analyzing the genotypes deposited in the GenBank and to define the range of those species. Materials and methods. Fin clips were sampled from 52 specimens of tubenose gobies collected from 2003 up to 2009 in 15 localities of the Ponto-Caspian basin. Genomic DNA for amplification of mitochondrial cyt b gene fragment (about 408 bp) was extracted using the Diatom®DNA Prep 100. The haplotype MedianJoining Network was constructed using the Network 4.5.1.0. software. Results. Tubenose gobies from the Black Sea basin are represented by two euryhaline species historically distributed in different areas: P. semilunaris in the north-western part of the Black Sea basin (and possibly eastern Aegean basin) and P. marmoratus in the north-eastern part. The hypothesis about freshwater and marine Black Sea Proterorhinus species seems to be not probable. The validity of endemic Crimean P. tataricus is not confirmed, several independent findings presume it to be conspecific with P. marmoratus. Some Crimean rivers and reservoirs are populated by P. semilunaris which could spread from the Dnieper River basin through the Severo-Krymskyi channel or may be accidentally introduced. The populations from the Caspian Sea and Sea of Azov basins represent the distinct evolutionary lineage of tubenose goby. But their taxonomic state should be analyzed by further molecular genetic studies of a wide scope of Caspian samples. Conclusion. The present data on the variability of the mitochondrial cyt b gene fragment in different tubenose goby populations result in revision of some previous taxonomic hypotheses in this fish group. At the same time further molecular genetic researches of Caspian populations seem to be the main tool for future development of taxonomic conclusions.
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