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Serum, livers and kidneys of 30 silver foxes from one breeding farm were subjected to analysis of the four microelements contents. The samples derived from 3 groups of animals (An=10) selected according to age and developed reproduction disorders. Cu, Cr and Mn were determined by a graphite furnace AAS whereas Zn by the flame AAS methods. Serum levels of Zn were the least variable (×(n=30)=4.72 ± 2.313 μg ·mL-1) and the mean of Cu content was 0.26 ± 0.244, of Cr was 0.029 ± 0.032 and of Mn was 0.074 ± 0.085 μg ·mL-1. The livers and kidneys contained respectively: 159.9 ± 23.66 and 74.25 ± 14.44 μg g⁻¹ of Zn; 34.03 ± 12.43 and 13.66 ± 1.67 μg g⁻¹ of Cu as well as 6.28 ± 0.97 and 2.60 ± 0.33 μg g⁻¹ of Mn. The most variable was Cr level achieving 1.00 ± 1.06 and 1.43 ± 2.64 μg g⁻¹ (all results per gram of wet weights). The differences between means and medians within the age groups did not exceed 41%, however chromium was an exception, its values differed more than 2 times. High zinc level found both in the serum and the organs resulted from its concentration in feedstuff being 1040,5 μg g⁻¹, exceeding the recommended level for farming foxes. Over-supplementation of dietary Zn might suggest connection between subtoxic action of Zn or its interaction with Cu or Cr followed by subsequent effect on reproduction events. Whether nature of Zn effects derived from direct target action or from Zn – Cu or Zn – Cr interactions have to be solved experimentally. Some foxes contained copper and chromium in livers and kidneys at levels exceeded significantly those concidered as physiological ranges for mammals, whereas manganese was within these limits. Thus, manganese was considered to keep homeostasis status of this element in the examined foxes.
The objective of the studies was to evaluate the influence of a natural preparation supplemented with unsaturated and saturated fatty acids on the clinical features and some biochemical indices in the mares in the last trimester of pregnancy and the first lactation months. The preparation efficacy was assessed in the prophylaxis of energy balance disturbances, with a special concern given to hyperlipaemia in horses. The preparation decreased the concentration of triglycerides (TG), total cholesterol, and cholesterol high density and low-density lipoprotein fractions. No significant changes were recorded in the levels of free fatty acids (FFA) and glucose. In the group of mares without the dietary addition, the TG and FFA levels exceeded the upper limits in some animals. The preparation had a beneficial effect on the energy balance in mares at the enhanced energy metabolism period.
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