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Background. The widespread and increasing occurrence of obesity, hypertension and associated disease has necessitated serial testing in order that risks of contracting such conditions become minimised through appropriate therapy and prevention. Many studies report that nutritional factors significantly affect the aetiology of hypertension and obesity that include mineral uptake. There are only a few studies however which are focused on the body’s changing mineral content during pharmaco-therapeutic treatment. Objectives. To determine concentrations of minerals in the hair and urine of hypertensive patients in conjunction with assessing their nutrition. Material and Methods. Subjects were 17 patients presenting with essential hypertension and 18 healthy controls. Atomic absorption spectrometry (AAS) was used to measure Mg, Ca, Fe, Zn and Cu in the hair and urine on a Zeiss AAS-3 instrument. Dietary mineral intakes were assessed by interview over 24 hours prior to the analysis. Results. The hypertensive group had significantly lower urine concentrations of Ca and Mg as well as Mg and Zn in hair. Urinary zinc excretion was significantly increased in this group compared to controls, but dietary intakes of Cu were reduced. The dietary mineral intakes were found to be unrelated to the concentrations of such minerals in the hair and urine. Conclusions. Compared to controls, excretion of Ca and Mg were reduced in hypertensive subjects, whereas Zn excretion was higher, and Mg and Zn were relatively low in the hair. Daily dietary intakes of Cu were also reduced in the hypertensive.
The field experiment (2009–2012) was conducted to assess the influence of different biofertilizers (AMF liquid/granular inocula, humic and seaweed extracts) on the growth and yielding of ‘Topaz’/M.26 apple planted on SARD soils in Poland. During conversion to organic orchard trees’ growth, fruit yield, their quality indices as well as nutritional status of leaf and fruit was ascertained. Fruit polyphenol content and their free radical scavenging activity were assessed. Moreover, the mycorrhizal root parameters (mycorrhizal and arbuscules frequency) were also presented. The most vigorous trees were inoculated with liquid inocula MicoPlant M and MicoPlant S. The plants treated with MicoPlant S gave the highest total yield (12.12 kg/tree) and revealed the best productivity (> 1 kg cm-2) as well as the average fruit weight. The liquid suspended inocula were more effective than granular one in terms of mycorrhizal root colonisation. Investigated biofertilizers increased P, K and Cu content of leaf. Organic soil extract (HumiPlant) decreased P and K content of fruit, while seaweed extracts (AlgaminoPlant) increased Ca amount of fruit. These treatments had the lowest K:Ca ratio. Used biofertilizers influenced apples polyphenol content as well as their antioxidant status.
‘Katja’ apple trees on M.9 Emla, M.9 751, M.9 984 and P 60 were planted on a silty loam alluvial soil, strongly fixing K, in autumn 1992. They were grown on non-irrigated and irrigated plots, with different potassium fertilisation applied since 1980. Leaf samples were taken in the years 1999–2001. Irrigation had not any significant effect on leaf mineral element content. However, a tendency to increasing leaf K concentration and decreasing Mg concentration in irrigated trees was noted. Fertilisation, irrespective of the dose and mode of application, significantly affected leaf mineral status. The annual doses of 200 kg K₂O·ha⁻¹ and cumulative dose of 800 kg K₂O·ha⁻¹, applied once in every four years, significantly increased K and P concentration. Leaves of non-fertilised trees contained more Mg. Leaves of P 60 contained more Ca and less Mg.
Retinoic acid-induced osteoporosis (RBM) is one of the most common causes of secondary osteoporosis. This study tested the anti-osteoporetic effect of quercetin in RBM-induced bone loss model (RBM). After 14-day supplementation of 13cRA to induce RBM, rats were administered with quercetin (100 mg/kg) or alendronate (40 mg/kg). We analysed changes in body and uterine weight of animals, femoral geometric characteristics, calcium and phosphorus content, bone weight index, bone hystology, bone mineral density (BMD), markers of bone turnover, lipid peroxidation, glutathione levels and SOD, CAT activity of liver, kidney spleen, and ovary as well as biochemical and haematological variables. In comparison to the control RBM rats, the treatment with quercetin increased bone weight index, BMD, osteocalcin level, femoral geometric characteristics, calcium and phosphorus content in the 13cRA-induced bone loss model. Histological results showed its protective action through promotion of bone formation. According to the results, quercetin could be an effective substitution for alendronate in 13cRA-induced osteoporosis. Good therapeutic potential of quercetin on rat skeletal system is based partly on its antioxidant capacity and estrogenic activity.
Studies concerning the effect of food consumption frequency on the mineral status in rheumatoid arthritis patients were carried out. The studies included 37 women with rheumatoid arthritis and 20 normal women serving as controls. The data relating to dietary preferences were collected from interview-based questionnaires. The copper and zinc content in serum samples were determined using the flame atomic absorption spectrometry (FAAS) technique.
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