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1

Newer facets of sickle cell adhesiveness

100%

Shohet S. B., Thevenin B. J. M., Sherman I. W., Andrian U.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 4

To further explore the finding that peptides derived from specifically exposed regions of band 3 inhibited sickle cell adhesiveness in vitro, we examined the effects of such peptides with human sickle cells in the mouse microcirculation in vivo. Human red cells were loaded with the fluorescent dye BCECF, and infused into the arterial circulation of anaesthetized mice. The microcirculation of several tissues was examined in a specially designed warm-stage microscope with stroboscopic epi-fluorescent illumination. Adhesive events were video-recorded and quantified after peptide infusion and after treatment of the mice with various biological modifiers. An adhesive index (AI), proportionate to the number of adhesive events per number of cells entering a vessel, was calculated. Sickle cells (SS) were found to be more adhesive than normal cells particularly in small vessels such as post-capillary venules of the cremaster muscle (AI 2.3 vs 0.5). Moreover, endothelium activation substantially increased SS adhesiveness (e.g., AI 8.3 after pretreatment with platelet activating factor (PAF), 5.8 with tumor necrosis factor, and 8.7 with thrombin-activated mouse platelets). A band 3 peptide which blocked SS adhesiveness in vitro inhibited much of the increased adhesiveness of SS cells (e.g., in the PAF-treated mouse AI 2.8 vs 8.3, vs pretreatment control 2.3). No inhibitory effect was seen with a control peptide with the same composition but a scrambled sequence. These preliminary findings suggest that the inhibitory activity of specific band 3 sequences on SS adhesiveness is retained in vivo and extend the previous in vitro demonstration of an adhesive role for these exposed band 3 sequences. In addition, they imply a substantial role for endothelial and/or platelet activation in the adhesiveness of sickle cells in vivo. Finally, they demonstrate the utility of direct visualization of the mouse microcirculation for studying the adhesiveness of human cells.

2

Production of prostacyclin and prostaglandin E2 in resting and IL-1beta-stimulated A549, HUVEC and hybrid EA.HY 926 cells

100%

Olszanecki R., Gebska A., Korbut R.

Journal of Physiology and Pharmacology

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2006

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tom 57

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nr 4

Production of arachidonic acid (AA) metabolites - prostacyclin (PGI2) in large vessels and prostaglandin E2 (PGE2) in microcirculation is intrinsically involved in maintenance of vascular wall homeostasis. EA.hy 926 is a hybrid cell line, is derived by fusion of HUVEC with A549 cells. The aim of this study was to examine the production of prostacyclin and PGE2 in resting and IL-1ß-stimulated EA.ha 926 cells, in comparison with its progenitor cells. Non-stimulated EA.hy 926 cells has been found to produce much lower amounts of prostacyclin than resting HUVEC. Resting hybrid cells produced more PGE2 than prostacyclin, despite they expressed high levels of COX-1 and PGI2 synthase. On the contrary to HUVEC and A549, EA.hy 926 cells did not respond to IL-1ß with COX-2 induction and increase of prostaglandin production, however they did it in response to lysophosphatidylcholine (LPC). The characteristics of EA.hy 926 cells in terms of the pattern of prostanoid formation could facilitate studies on endothelial metabolism and role of these important lipid mediators.

3

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Lung thrombopoiesis in pregnant rabbits during septic shock - an ultrastructural study

100%

Sulkowska M., Terlikowski S., Famulski W., Sulkowski S.

Bulletin of the Veterinary Institute in Puławy

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2001

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tom 45

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nr 1

The aim of the present study was the comparative analysis of morphological changes found in the pulmonary microcirculation of pregnant rabbits in the course of experimental septic shock induced by endotoxin administration. Sixteen female rabbits, white Dutch, c.3 kg of mean body weight were used in the experiments. The endotoxin of Escherichia coli, serotype S.0127:138 (Sigma) was applied intraperitoneally in a single dose of 100 μg/kg b.w. Morphological examinations were based on the ultrastructural analysis with the transmission electron microscope. Severe damage of endothelial cells (necrosis inclusive) was observed in endotoxin-treated rabbits. The vascular lumen in these animals showed cellular aggregation of neutrophils and platelets in particular, as well as microthrombi. Some of the blood vessels showed fragments of megakaryocytes. An increased tendency towards the development of these changes was noted in pregnant rabbits. The study confirmed that the lungs may be an important site of extramedullary thrombopoiesis in the course of septic shock especially during pregnancy.

4

Clinical evaluation of the microcirculation in health and disease

100%

Fagrell B.

Journal of Physiology and Pharmacology. Supplement

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1993

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tom 44

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nr 2

5

Neurogenic and myogenic resting skin blood flowmotion in subjects with masked hypertension

84%

Gryglewska B., Necki M., Cwynar M., Baron T., Grodzicki T.

Journal of Physiology and Pharmacology

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2010

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tom 61

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nr 5

6

Temperature distribution in rheumatoid hand after standard infrared treatment

84%

Rutkowski R., Straburzynska-Lupa A., Korman P., Romanowski W., Raglewska P., Konarska A., Gizinska M., Hurnik E., Czubaszewski L.

Trends in Sport Sciences

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2013

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tom 20

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nr 1

Introduction. Comprehensive therapy of rheumatoid arthritis (RA), apart from pharmacological treatment, also necessitates an implementation of specialized procedures aimed at improving the mobility, stamina and strength of affected joints. Physical treatments used for the rehabilitation of patients with RA are most often related to thermal stimuli and change the temperature of the tissues. Each change of that kind is linked to the adequate vascular reaction and the changes in blood circulation within the affected area. Aim of the Study. The aim of this present study was to examine vascular changes in rheumatoid hand occurring as a physiological response to the mild thermal stimulus being applied, accounting for individual differences in its progress. Material and Methods. The research embraced 32 patients aged 54.9 ± 6.8 with diagnosed RA according to the standards of American College of Rheumatology. For physical therapy a conventional infrared lamp emitting A, B, C waves with a red colour filter was used. The area under treatment was the dorsal side of the hand. Each subject had eight thermographic pictures taken at the pre-defined time intervals: before the application, immediately after the application, and 5, 15, 30, 45, 60 and 120 minutes after the application. Results. The results were shown in a form of graphic reaction progress of the heated and unheated (contralateral) hand. Average static temperatures of both hands did not show any differences (31.8 ± 1.7°C – heated hand; 31.9 ± 1.8°C – unheated hand). Maximum temperature was obtained immediately following the IR lamp application: 35.0 ± 1.2°C for the heated hand and 32.2 ± 2.1°C for the unheated one. Among all analyzed diagrams showing reaction progress following IR application, four individual groups with the most similar results were formed. Conclusions. For all the patients in the study, a comparable decrease in tissue temperature initially increased by IR application was noted within the next 45 minutes following the application. No subject observed any undesirable reactions.

7

Mechanisms of vascular dysfunction after subarachnoid hemorrhage

84%

Kozniewska E., Michalik R., Rafalowska J., Gadamski R., Walski M., Frontczak-Baniewicz M., Piotrowski P., Czernicki Z.

Journal of Physiology and Pharmacology. Supplement

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2006

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tom 57

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nr 11

145-160

The main consequence of subarachnoid hemorrhage, for those who survive bleeding, is delayed, persistent vasospasm of intracranial conduit arteries which occurs between the third and seventh day after the insult and results in symptomatic brain ischemia in about 40% of cases. This vasospasm is considered to be a major cause of disability of post-SAH patients. Despite extensive experimental and clinical research, mechanisms of vasospasm are not fully understood. Dysfunction of the endothelium resulting in enhanced production of vasoconstrictors, phenotypic changes of the receptors in endothelium and smooth muscle cells, increased sensitivity of vascular smooth muscle cells to vasoconstrictors, release of spasmogens from lysed blood clot and inflammatory response of the vascular wall have been demonstrated and discussed as pathological mechanisms participating in the development of spasm. In recent years more attention is paid to the functional and structural changes in microcirculation and a concept of microvascular spasm is evolving. Our experimental studies in rat model of SAH strongly suggest that microcirculatory dysfunction and delayed vasospasm are related to the severity of acute, transient ischemia caused by critical decrease of perfusion pressure and active vasoconstriction immediately after the bleeding.

8

Microangioarchitectonics of the myocardial venous vessels in coronary heart disease

84%

Ratajczyk-Pakalska E., Bloch P., Kulig A.

Folia Morphologica

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1990

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tom 49

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nr 1-2

9

Microcirculatory disturbances in caerulein-induced acute pancreatitis in rats with reference to nitric oxide, heparin and procaine treatment

84%

Dobosz M., Hac S., Wajda Z., Rzepko R., Roszkiewicz A., Zawrocka-Wrzolek T.

Journal of Physiology and Pharmacology. Supplement

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1998

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tom 49

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nr 2

10

Nitric oxide, heparin and procaine treatment in experimental ceruleine-induced acute pancreatitis in rats

84%

Dobosz M., Wajda Z., Hac S., Mysliwska J., Bryl E., Mionskowska L., Roszkiewicz A., Mysliwski A.

Archivum Immunologiae et Therapiae Experimentalis

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1999

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tom 47

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nr 3

11

Role of the endogenous nitric oxide in the vasodilatory tone and CO2 responsiveness of the rostral ventrolateral medulla microcirculation in the rat

84%

Wolk R., Nowicki D., Sieminska J., Trzebski A.

Journal of Physiology and Pharmacology

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1995

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tom 46

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nr 2

12

Glutamine and alanyl-glutamine dipeptide reduce mesenteric plasma extravasation, leukocyte adhesion and tumor necrosis factor-alpha [TNF-alpha] release during experimental endotoxemia

67%

Scheibe R., Schade M., Grundling M., Pavlovic D., Starke K., Wendt M., Retter S., Murphy M., Suchner U., Spassov A., Gedrange T., Lehmann C.

Journal of Physiology and Pharmacology. Supplement

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2009

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tom 60

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nr 8

19-24

13

Specific features and roles of renal circulation: angiotensin II revisited

67%

Sadowski J., Badzynska B.

Journal of Physiology and Pharmacology. Supplement

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2006

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tom 57

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nr 11

169-178

The status of intrarenal circulation determines in part renal excretion, affects body fluid homeostasis and has a role in long term control of arterial blood pressure. The vascular resistance in the renal cortex and medulla is determined by interaction of a vast array of vasoactive hormones and paracrine factors; among these the role of constrictor angiotensin II and dilator prostaglandins and nitric oxide may appear to be dominating. The focus of this review and underlying studies is on the mechanisms whereby the microcirculation of the renal medulla is protected against the vasoconstrictor action of angiotensin II. In anaesthetized normal rats the three mentioned active agents or their inhibitors were applied and total renal blood flow and cortical, outer- and inner medullary laser-Doppler fluxes were determined; in some studies renal tissue nitric oxide was measured using selective electrodes. We conclude that angiotensin II, acting via AT1 receptors, constricts the renal cortical vasculature; in the medulla its action is effectively buffered by prostaglandin E2 but most probably not by nitric oxide.

14

Impairment of microcirculation in juvenile idiopathic arthritis - studies by nailfold videocapillaroscopy and correlation with serum levels of sICAM and VEGF

67%

Gorska A., Kowal-Bielecka O., Urban M., Chlabicz S., Sienkiewicz J., Gorski S.

Folia Histochemica et Cytobiologica

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2008

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tom 46

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nr 4

443-447

15

The expression of the renin-angiotensin-aldosterone system in the skin and its effects on skin physiology and pathophysiology

67%

Aleksiejczuk M., Gromotowicz-Poplawska A., Marcinczyk N., Przylipiak A., Chabielska E.

Journal of Physiology and Pharmacology

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2019

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tom 70

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nr 3

16

Effect of endotoxin on protein degradation and lipid peroxidation of erythrocytes

67%

Bhattacharyya J., Chowdhury T. D., Datta A. G.

Journal of Physiology and Pharmacology

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1999

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tom 50

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nr 2

17

Effects of cervical sympathetic nerve stimulation on the cerebral microcirculation: possible clinical implications

67%

Passatore M., Deriu F., Roatta S., Grassi C., Micieli G.

Acta Neurobiologiae Experimentalis

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1996

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tom 56

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nr 1

18

Role of heat shock proteins in gastric inflammation and ulcer healing

59%

Choi S. R., Lee S. A., Kim Y. J., Ok C. Y., Lee H. J., Hahm K. B.

Journal of Physiology and Pharmacology. Supplement

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2009

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tom 60

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nr 7

Ograniczanie wyników

Newer facets of sickle cell adhesiveness

Production of prostacyclin and prostaglandin E2 in resting and IL-1beta-stimulated A549, HUVEC and hybrid EA.HY 926 cells

Lung thrombopoiesis in pregnant rabbits during septic shock - an ultrastructural study

Clinical evaluation of the microcirculation in health and disease

Neurogenic and myogenic resting skin blood flowmotion in subjects with masked hypertension

Temperature distribution in rheumatoid hand after standard infrared treatment

Mechanisms of vascular dysfunction after subarachnoid hemorrhage

Microangioarchitectonics of the myocardial venous vessels in coronary heart disease

Microcirculatory disturbances in caerulein-induced acute pancreatitis in rats with reference to nitric oxide, heparin and procaine treatment

Nitric oxide, heparin and procaine treatment in experimental ceruleine-induced acute pancreatitis in rats

Role of the endogenous nitric oxide in the vasodilatory tone and CO2 responsiveness of the rostral ventrolateral medulla microcirculation in the rat

Glutamine and alanyl-glutamine dipeptide reduce mesenteric plasma extravasation, leukocyte adhesion and tumor necrosis factor-alpha [TNF-alpha] release during experimental endotoxemia

Specific features and roles of renal circulation: angiotensin II revisited

Impairment of microcirculation in juvenile idiopathic arthritis - studies by nailfold videocapillaroscopy and correlation with serum levels of sICAM and VEGF

The expression of the renin-angiotensin-aldosterone system in the skin and its effects on skin physiology and pathophysiology

Effect of endotoxin on protein degradation and lipid peroxidation of erythrocytes

Effects of cervical sympathetic nerve stimulation on the cerebral microcirculation: possible clinical implications

Role of heat shock proteins in gastric inflammation and ulcer healing