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To protect materials against biodetenoration, physical, biological or chemical methods can be used. Chemical inhibitors of biodetenoration are the most common and effective. A new class of chemical inhibitors-gemini alkylammonium salts-shows excellent biocidal properties and good ecological profile. These compounds can be applied as biodeterioration inhibitors in a wide variety of materials.
Earthworms, as ecosystem engineers, strongly regulate microbial activities and microorganism-mediated processes in the soil; their effects differ among species and ecological groups. Lumbricids are suggested to have density-dependent regulation of species populations, but it is not known whether their effects on soil processes are density-dependent. In a field experiment, litter/soil microcosms contained monocultures of five common lumbricid species belonging to epigeic, anecic and endogeic ecological groups, at various density levels. After 6 and 15 weeks, respiration rates of soil systems were measured and (after subtraction of approximately calculated earthworm respiration) microbial respiration rates estimated. In the presence of earthworms, respiration of soil systems tended to increase. After 6 weeks, this increment was explained by earthworm's own respiration. However, after 15 weeks earthworm respiration comprised 12–80% of the respiration increment; hence, microbial respiration was stimulated by earthworm activities. In any earthworm species, total community and microbial respiration were correlated with its density increase. However, specific respiration increments (per unit earthworm biomass) were not significantly affected by lumbricid density. The lack of density-dependent patterns indicates a weak impact of lumbricid intraspecific interactions on soil respiration. However, specific respiration increments and stimulation of microbial respiration varied across earthworm species, being higher for endogeic than for epigeic/anecic species. This is explained by a relatively lower microbial grazing by endogeics and a depletion of litter (a resource and environment for the microbial community) by epigeic/anecic earthworms. Overall, the results support the view that microbial community adapts to the presence of earthworms by switching to a smaller, but a more active one.
Field study was performed on Haplic Luvisol soil to determine the effects of soil compaction and straw mulching on microbial parameters of soil under soybean. Treatments with different compaction were established on unmulched and mulched with straw soil. The effect of soil compaction and straw mulching on the total bacteria number and activities of dehydrogenases, protease, alkaline and acid phosphatases was studied. The results of study indicated the decrease of enzymes activities in strongly compacted soil and their increase in medium compacted soil as compared to no-compacted treatment. Mulch application caused stimulation of the bacteria total number and enzymatic activity in the soil under all compaction levels. Compaction and mulch effects were significant for all analyzed microbial parameters (P<0.001).
To evaluate the small-scale (100 m²) spatial variability of selected soil biological properties in a beech stand and the influence of soil properties on soil microbial activity, we collected soil samples on April 2, July 2 and October 30 (2002) from three (9 ¥ 10 m) plots, distributed on a 1 ¥ 1 m grid, from the top 10 cm of the mineral soil (A horizon). In soil samples, soil respiration and catalase activity were measured. Within-plot coefficients of variation of catalase activity varied from 19 to 23%, those of soil respiration rate ranged from 37 to 54%. Catalase activity exhibited highly significant correlations with soil reaction, whereas soil respiration rate correlated with moisture and humus content. Soil respiration rates appear to be randomly distributed over the sampling area. On the other hand, catalase activity exhibits a rather distinct patchy structure. No relationships between the position of trees and soil respiration rates were found. For catalase activities, the highest values were observed on transsections of perimeters of dominant-tree crowns.
The effects of Cu on the production of hydroxamate siderophores, microbial activity and physiological state of bacteria in a sandy-loam soil were studied. Soil samples were treated with either 0.5 or 2.5 mg Cu (as chloride) g-1 or were left untreated. Soil microflora responded to added Cu by changing the physiological state and production of specific metabolites, i.e. siderophores; however, microbial activity did not show any significant change.
Soil microbes' activity is very important for forming of the nutrient stock and , soil structure, as well as the carbon cycle simulation. This is particularly crucial for deep soil layers. Effect of soil microbes on the rate of accumulation and decomposition of the soil organic carbon (SOC) has been found for different regions. However, it is known still a little on the SOC performance for different decomposition rates and its relation to the microbial activity in the saline-alkali desert ecosystem. Therefore, the main task of our research was investigation of interrelation between the soil organic carbon and microbial carbon (SMC) at different depths in the original saline-alkali Gurbantünggüt Desert. Our results showed in the soil vertical profile, (i) SMC and SOC presented a very significant positive linear correlation (R² = 0.63, P = 0.0003); (ii) SMC exhibited two obvious changed-interfaces - 20 cm and 80 cm, the SMC at depth of 0–20 cm, 20–80 cm and 80–500 cm was 2.24–3.06, 0.19–0.72, and 0.0017–0.0097 mg kg⁻¹, respectively; (iii) in the depth of 0–20 cm and 20–80 cm, the SMC had highly significant difference (P < 0.0001) and at 20–80 cm and 80–500 cm, significant difference (P = 0.013); (iv) according to the soil division based on the SMC, SOC also had some certain stratification; (v) organic carbon layers can be respectively defined according to different microbial activities as active, inert, and stable organic carbon pool. Therefore, these three kinds of organic carbon pools can be quantitatively measured by analyzing their location at different depths of the soil profile.
The effects of the colonization of an abandoned grassland by forest trees and the associated microenvironmental changes on soil properties and the microbial activity of soil were studied along a 170 m transect established on a former pasture colonized by Norway spruce (Picea abies Karst.) in Central Slovakia. The transect with a 5×5 m grid of sampling points crosses all successional stages from a closed forest to an open grassland, representing 50 years of secondary succession. Changes of basal soil respiration, substrate-induced respiration and soil catalase activity in association with tree density, light and temperature regime were examined, and their relationships with chemical soil properties were documented. Generally, light conditions, tree influence and soil temperatures were highly significantly correlated with the distance from the lower transect edge, but the correlation coefficients were not very high (|r| ranged between 0.37 and 0.70), indicating that the microsite conditions within the transect are strongly heterogeneous. With the advance of colonization, the amount of soil organic matter was found to increase, probably in association with a higher amount and a lower quality of litter. Catalase activity exhibits a linear trend along the transect, which is explained by a lower direct insolation of soils (r = 0.28***) and decreasing temperatures (r = 0.36***) in the upper part. Basal respiration is the lowest in the middle of the transect, where colonizing spruces form a dense closed stand with soil covered by a thick layer of raw litter. Both in the lower part covered by species-rich grassland communities and in the upper part, where forest herb species begin to establish, basal respiration is higher, probably due to higher amounts of easily decomposable plant material. In addition to these transect-wide trends, stationary spatial patterns were observed for most soil variables. Generally, microbial activity rates as well as chemical soil properties exhibited spatial continuity up to the distance of approx. 40 to 60 m.
The influence of crumbled sewage sludge on the biological properties of podzolic soil was studied under laboratory conditions during a five-month period. It was found that when compared to only slightly crumbled sludge, soil mixed with two different concentrations of crumbled sludge (1% and 10%) of a 0.5-mm particle diameter exhibited an increase in the numbers of all tested bacteria groups (total, lipolytic, cellulolytic, proteolytic). The stimulation of development of some fungi groups (cellulolytic, proteolytic) was also observed in soil with higher contents of crumbled sludge. Finally, strongly crumbled sludge stimulated most of the biochemical activities of the soil (ammonification, nitrification, dehydrogenases, lipase, protease). Such an effect was most evident in the cases of dehydrogenases and protease.
The paper describes a field experiment with the application of two biocides: oxytetracycline that reduced bacteria and captan that reduced soil fungi. The purpose of the experiment was to investigate the response of enchytraeid species to the changes of the abundance and activity of the microbial community as part of their food resources. The following variables were recorded: the biomass of microorganisms by the PLFA method, the rate of cellulose decomposition and the numbers and species composition of enchytraeids. No relationship was found between enchytraeids and abundance of microorganisms, but numbers of animals were correlated with changes in soil cellulolytic activity. Both biocides, even the non toxic oxytetracycline, reduced the diversity (H’) of enchytraeid community by increasing the proportion of dominant species. It is suggested that biocides reduced the functional diversity of microorganisms, and this factor had an effect on the enchytraeid populations. The animals responded to the treatment and their community became predominated by the species probably with a broad food spectrum.
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