Wyniki wyszukiwania - AGRO

1

Mycelial culture of Xerocomus chrysenteron and its metabolites

100%

Kohlmunzer S., Wegiel J., Grzybek J.

Acta Mycologica

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1998

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tom 33

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nr 2

2

The effect of metal ions and metabolites on the activity of pyruvate kinase from bean roots

100%

Jezewska M. J., Flieger M.

Applied Biology Communications. Lublin Scientific Center

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1992

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tom 02

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nr 4

3

Changes in the uptake of selected metabolites in relation to differentiation in Brassica napus callus cultures

100%

Zur I., Skoczowski A., Pienkowski S.

Acta Physiologiae Plantarum

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1997

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tom 19

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nr 2

4

The X-ray structure and absolute configuration of the anticancer drug (plus)-4-ketocyclophosphamide

86%

Gdaniec M., Adamiak D. A., Misiura K.

Acta Biochimica Polonica

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1989

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tom 36

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nr 3-4

5

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Vitamin B6 and its metabolite reveal radical scavenging activity at basic pH

86%

Gliszczynska-Swiglo A., Szymusiak H.

Polish Journal of Food and Nutrition Sciences

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2007

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tom 57

|

nr 4[A]

163-167

In the present study, the pH-dependent radical-scavenging activities, in the TEAC (Trolox equivalent antioxidant capacity) assay, of pyridoxine (PN), pyridoxal (PL), pyridoxamine (PM) (vitamin B6 forms) and their main metabolite 4-pyridoxic acid (PA) was determined. For all compounds, the increase in the TEAC values with increasing pH of the medium from value 7 to 9 was observed. The order of radical-scavenging activity was found as follows: PN>PM>PL>PA and it was confirmed by their oxidative potentials measured using cyclic and square-wave voltammetry. Quantitative relationship between experimental radical-scavenging activity and calculated bond dissociation energy values (BDE) was found suggesting hydrogen atom donation as the main mechanism of radical-scavenging activity of vitamin B6 and PA. The results of the present study suggest that vitamin B6 may contribute to the total antioxidant capacity of antioxidants present in human body especially at basic conditions.

6

Formation of lipid droplets induced by 2,3-dihydrogeranylgeranoic acid distinct from geranylgeranoic acid

86%

Kodaira Y., Kusumoto T., Takahashi T., Matsumura Y., Miyagi Y., Okamoto K., Shidoji Y., Sagami H.

Acta Biochimica Polonica

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2007

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tom 54

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nr 4

777-782

Geranylgeranoic acid (GGA) and 2,3-dihydrogeranylgeranoic acid (2,3-diGGA) are geranylgerani-ol-derived metabolites (Kodaira et al.(2002) J Biochem132:327–334). In the present study, we examined the effects of these acids on HL-60 cells. The cells were differentiated into neutrophils by GGA stimulation like retinoic acid stimulation. In the case of cells stimulated with 2,3-diGGA, neutrophils were not detected, but the formation of lipid droplets was induced. On the other hand, when the cells were cultured in the presence of 0.1% FBS instead of 10% FBS, apoptotic cells were induced not only by GGA stimulation but also with 2,3-diGGA. In the latter case, when the cells were cultured in the co-presence of a caspase-3 inhibitor (Ac-DMQD-CHO), the lipid droplets formation was observed in the cells. These results suggest that GGA and 2,3-diGGA are extremely different from each other with respect to their effects on HL-60 cells.

7

Nucleotide metabolism in an Italian APRT deficient patient

86%

Micheli V., Jacomelli G., Barberi S., Burroni B., Di Marcello F., Notarantonio L., Peruzzi L., Mazzaferro S., Pompucci G.

Cellular and Molecular Biology Letters

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1999

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tom 04

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nr 3

8

Determination of metoprolol and its metabolites in human urine by capillary isotachophoresis

86%

Sadecka J., Polonsky J., Shintani H.

Polish Journal of Environmental Studies

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1997

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tom 06

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nr 2

The separation and determination of metoprolol and its metabolites M I (metoprolol acid), M II (2-hydroxy-3-(4-metoxyethylphenoxy)-propanoic acid, M III (a-hydroxymetoprolol) and H 105 (O-desmethylmetoprolol) in human urine by capillary isotachophoresis were investigated. Metoprolol and metabolites M I, M III and H 105 were separated by cationic isotachophoresis in the electrolyte system sodium acetate buffer (pH 5.0) (c,=10 mM) -ß-alanine. Metabolite M II was separated using the anionic electrolyte system histidine hydrochloride buffer (pH 6.0) (cL = 10 mM) -morpholinoethane-sulfonic acid. Endogenous and the possible exogenous compounds were almost totally removed from urine by solid-phase extraction using a SepPak Cls cartridge. The recovery of compounds varied from 84.6 to 95.8%. The linear calibration range was studied for eventual application of the method to real urine samples. Limit of determination was 0.5 mg/ml.

9

Persistent organic pollutants [POPs] in bottom sediments from the Baltic Sea

86%

Sapota G.

Oceanological and Hydrobiological Studies

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2006

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tom 35

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nr 4

10

Influence of polyamines on growth and formation of secondary metabolites in hairy root cultures of Beta vulgaris and Tagetes patula

86%

Bais H. P., Madhusudhan R., Bhagyalakshmi N., Rajasekaran T., Ramesh B. S., Ravishankar G. A.

Acta Physiologiae Plantarum

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2000

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tom 22

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nr 2

11

Secondary metabolites as Co-markers in the taxonomy of Aspergilli

86%

Abu-Seidah A. A.

Acta Microbiologica Polonica

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2003

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tom 52

|

nr 1

12

The effect of amphiphilic biocydes contained in a medium on liposome membrane fluidity

86%

Podolak M., Waga S., Przestalski S.

Polish Journal of Environmental Studies

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1994

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tom 03

|

nr 2

It was found that quaternary ammonium salt derivatives of glycine ester (GE) admixtures induced increased fluidity of egg yolk lecithin (EYL) liposome membranes (L). The increase depended on chain-lengths of the GEs added and on their concentrations. A maximum fluidity was observed with the 12-carbon atom compound. From considerations concerning the electrostatic interactions in the hydrophilic layer of the liposome mebranes it follows that GEs at low concentrations can induce an increase of binding energy of the layer, while at hihger concentrations the opposite effect can occur.

13

Effect of energy restriction on serum cortisol and its faecal metabolite [11,17-dioxoandrostan] in pregnant ewes

86%

Ates A., Altiner A., Ozpinar A., Mostl E.

Bulletin of the Veterinary Institute in Puławy

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2008

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tom 52

|

nr 3

The objective of the study was to investigate the effect of an energy restriction on serum cortisol and its faecal metabolite (11,17-dioxoandrostan) in Chios ewes in late gestation. Twenty pregnant and ten non-pregnant out of 38 randomly selected ewes were detected with ultrasound and were separated into three groups (n=10) on day 105 after mating: normal energy fed pregnant group (NE), restricted energy fed pregnant group (RE), and non-pregnant group (NP). Blood samples were taken on days 117, 130, and 144 of pregnancy and faecal samples were taken twice a week starting on day 106 of pregnancy from both pregnant and non-pregnant ewes. After extraction of serum and faecal samples, concentrations of cortisol and 11,17-dioxoandrostan (11,17- DOA) were measured. A significant decrease in RE was determined only on day 144 of pregnancy (P≤0.05). Between the two pregnant groups (NE and RE), a significant difference was found only on day 144; at this period, RE had lower serum concentrations. RE had significantly lower 11,17-DOA concentrations in late pregnancy than NE. In pregnant ewes, the levels decreased in a late gestational period. The present data shows that in ewes fed 14.47% crude protein and 8.82 MJ /kg of metabolic energy in late pregnancy, pregnancy toxaemia does not occur. We suggest that the determination of Cortisol metabolites in faeces is more objective and safe than that of serum Cortisol concentrations.

14

Pharmacokinetics of tramadol and metabolites after injective administrations in dogs

72%

Giorgi M., Del Carlo S., Lebkowska-Wieruszewska B., Kowalski C. J., Saccomanni G.

Polish Journal of Veterinary Sciences

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2010

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tom 13

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nr 4

The aim of this study was to determine the pharmacokinetics of tramadol and its main metabolites after IV and IM injections. The pharmacokinetic cross-over study was carried out on 6 healthy male beagle dogs. Tramadol was administered by intravenous (IV) and intramuscular (IM) injection at 4 mg/kg. Tramadol and its main metabolites O-desmethyl-tramadol (M1), N-,N-didesmethyl-tramadol (M2) and N-,O-didesmethyl-tramadol (M5) concentrations were measured in plasma samples by a HPLC coupled with fluorimetric detection; pharmacokinetic evaluations were carried out with a compartmental and non-compartmental model for tramadol and its metabolites, respectively. The bioavailability of the drug, ranging between 84-102% (mean 92%), was within the generally accepted values for a positive bioequivalence decision of (80-125%). After the IM injection the mean plasma drug concentration peak was reached after a Tmax of 0.34 h with a Cmax of 2.52 μg/mL. No therapeutic relevant differences were observed between IM and IV administration. The minimal effective plasma concentration was reached after a few minutes and maintained for about 6-7 h in both administrations. M1 plasma concentration was low and the amounts of the other metabolites produced were analogous in both routes of administration. In conclusion, tramadol was rapidly and almost completely absorbed after IM administration and its systemic availability was equivalent to the IV injection. The different onset time and duration of action observed were very small and probably therapeutically irrelevant. The IM injection is a useful alternative to IV injection in the dog.

15

Using 13C labeled glucose to investigate glutamate metabolism

72%

Sonnewald U., Brekke E., Walls A., Waagepetersen H., Schousboe A.

Acta Neurobiologiae Experimentalis

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2013

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tom 73

|

nr 1

Using 13C labeled compounds and 13C magnetic resonance spectroscopy (MRS) it is possible to monitor cellular metabolism and astrocyte-neuronal interactions. Various 13C labeled substrates are used to unravel different aspects of cerebral metabolism. This presentation will focus on [1-13C]glucose, [U-13C] glucose, [2-13C]glucose and [3-13C]glucose metabolism in cerebellar and cerebro-cortical neurons and astrocytes in culture. [1-13C]Glucose is metabolized by both astrocytes and neurons and labeling of metabolites from this isotopomer of glucose will not be affected by the pentose phosphate pathway (PPP). Using [U-13C]glucose and 3-nitropropionic acid it could be confirmed that pyruvate carboxylation takes place in cortical astrocytes but not neurons. This carboxylation leads to the formation of oxaloacetate, which condenses with acetyl coenzyme A to form citrate. However, oxaloacetate may also be converted to malate and fumarate before being regenerated. This redundant pathway is termed the oxaloacetate-fumarate-flux, or backflux and has been shown to be extensive using [2-13C]- and [3-13C]glucose in cultured cerebral cortical and cerebellar cultures. It could also be calculated to be present in vivo. [2-13C]- and [3-13C]glucose can also be used to probe the PPP in neurons where pyruvate carboxylation is not present. Indeed, the PPP contributed to labeling of glutamate and other metabolites.

16

Determination of Carbofuran and its metabolites in plant tissues by electron capture gas chromatography

72%

Dec J., Czaplinski E.

Prace Naukowe Instytutu Ochrony Roślin

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1980

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tom 22

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nr 2

17

Dietary conjugated linoleic acid isomers and selenium affect the profile of conjugated linoleic acid isomers and their metabolites in abdominal fat of rats

72%

Czauderna M., Kowalczyk J., Korniluk K.

Polish Journal of Natural Sciences. Supplement

|

2006

|

tom 03

563-568

18

Fate of dietary phytosteryl/-stanyl esters upon digestion by humans: analysis of intact esters and their metabolites in feces

72%

Lubinus T., Barnsteiner A., Engel K.-H.

Polish Journal of Food and Nutrition Sciences

|

2011

|

tom 61

|

nr Suppl.1

19

Anaerobic processes in yellow lupin (Lupinus luteus L.) root nodules

72%

Mazurowa H., Ratajczak L.

Acta Biochimica Polonica

|

1989

|

tom 36

|

nr 3-4

20

Identification of 2-[2-nitro-4-[trifluoromethyl]benzoyl]-cyclohexane-1,3-dione metabolites in urine of patients suffering from tyrosinemia type I with the use of1H and 19F NMR spectroscopy

72%

Szczecinski P., Lamparska D., Gryff-Keller A., Gradowska W.

Acta Biochimica Polonica

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2008

|

tom 55

|

nr 4

749-752

Organic extracts of six urine samples from children treated with nitisinone, a medicine against tyrosinemia type I, were investigated by 1H and 19F NMR spectroscopy. The presence of unchanged 2-[2-nitro-4-(trifluoromethyl)benzoyl]cyclohexane-1,3-dione (NTBC), 6-hydroxy-2-[2-nitro-4-(trifluoromethyl)benzoyl]cyclohexane-1,3-dione (NTBC-OH) and 2-nitro-4-trifluoromethylbenzoic acid (NTFA) as well as a few other unidentified compounds containing CF3 group was documented.

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