Wyniki wyszukiwania - AGRO

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Optimization of the medium for Lactobacillus acidophilus by Plackett-Burman and steepest ascent experiment

100%

Chen H., Niu J., Qin T., Ma Q., Wang L., Shu G.

Acta Scientiarum Polonorum. Technologia Alimentaria

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2015

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tom 14

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nr 3

Background. Lactobacillus acidophilus not only improves the intestinal flora balance but also inhabits the growth of undesirable microorganisms in intestine, which is benefit to the health of humans and animals. Plackett-Burman and steepest ascent experiment are the rapid and concise ways of screening the main effective factors. This study is aimed to select the main influence factors and optimize the medium for Lactobacillus acidophilus by Plackett-Burman experiment and steepest ascent experiment. Material and methods. The ideal carbon source was screened among glucose, maltose, lactose and whey powder, and the ideal nitrogen source was screened among casein hydrolysate, peptone, yeast extract powder, fish meal, carbamide, ammonium sulfate and sodium nitrate by single factor experiment. Plackett-Burman and steepest ascent experiment were applied to screen the main effective factors of Lactobacillus acidophilus among peptone, beef extract, yeast extract powder, glucose, K2HP04, C6H1407N2, CH3COONa, MgS04 and Tween-80. Result. The results indicated that glucose (p = 0.01510) as negative factor and K,HP04 ip = 0.02017) as positive effect were the significant growth factors of Lactobacillus acidophilus, CH3COONa (p = 0.09273) as positive effect was an important factor, and the optimized medium was as follows: glucose - 21 g/L, K2HP04 - 3.5 g/L, CH3COONa - 6.5 g/L, peptone - 10 g/L, beef extract - 8 g/L, yeast extract powder - 8 g/L, C6H1407N2 - 2 g/L, MgS04 - 0.2 g/L and Tween-80 - 1 mL/L when the maximum viable count could achieve 2.72* 109 cfii/mL. Discussion. The experimental model is reliable and the experimental results are of good stability. Variance analysis is performed to determine the adequacy and significance of the linear model. Thus, Plackett-Burman and steepest ascent experiment improve the veracity of optimization the medium for Lactobacillus acidophilus compared with the previous research.

2

The influence of different media on the Laetiporus sulphureus [Bull.:Fr.] Murr. mycelium growth

100%

Siwulski M., Pleszczynska M., Wiater A., Wong J.-J., Szczodrak J.

Herba Polonica

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2009

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tom 55

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nr 3

278-284

The aim of the research was to determine the effect of medium on the mycelium growth of L. sulphureus. The subject of the studies were six L. sulphureus strains: LS02, LS206, LS286, LS302, LSCNT1 and LSCBS 388.61. Eight different agar media and six solid media were used in the experiment. Some morphological characteristics of mycelium on agar media were also evaluated. It was found that PDA was the best agar medium for mycelium growth of all tested strains. The strain LS302 was characterized by very high growth rate regardless of the examined agar medium. The tested strains presented changes in mycelium morphology on different agar media. The best mycelium growth was obtained on alder, larch and oak sawdust media, mainly in LSCBS 388.61, LS02 and LS286 strains.

3

Lipid composition and lectin-mediated agglutination of Candida kefyr cells grown in media enriched with choline or its analogues

100%

Sharma S. C., Singh B.

Acta Biochimica Polonica

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1989

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tom 36

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nr 3-4

4

Automatyzacja personalizacji reklamy internetowej

100%

Borusiak B., Pieranski B., Romanowski R., Strykowski S.

Marketing i Rynek

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2015

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tom 22

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nr 03

5

Effect of cultivation conditions on Monilia coryli growth and sporulation

100%

Zalewska E.

Phytopathologia Polonica

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2001

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tom 22

6

Shoot cultures of strawberry cv. Syriusz

100%

Malodobry M., Dziedzic E., Lech W.

Folia Horticulturae

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1997

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tom 09

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nr 1

7

Media for efficient generating nitrate-nonutilizing [nit] mutants of Verticillium dahliae, Colletotrichum gloeosporioides, Colletotrichum lindemuthianum and Fusarium oxysporum

100%

Rataj-Guranowska M., Pieczul K., Nowak E., Hiemstra J. A., Drapikowska M.

Phytopathologia Polonica

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2001

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tom 22

8

Mechanizm oddziaływania mediów reklamy – cz. 1

100%

Czarnecki A.

Marketing i Rynek

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1999

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tom 06

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nr 03

9

Carbohydrates content in Clematis shoots cultured in vitro depending on temperature and sucrose/nitrogen level in the medium

84%

Kawa-Miszczak L., Wegrzynowicz-Lesiak E., Gabryszewska E., Saniewski M.

Acta Physiologiae Plantarum

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2007

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tom 29

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nr 1 Suppl.

10

Effect of temperature and sucrose/nitrogen level in the medium on chlorophyll and anthocyanin contents in Clematis cultured in vitro

84%

Kawa-Miszczak L., Wegrzynowicz-Lesiak E., Gabryszewska E., Saniewski M.

Acta Physiologiae Plantarum

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2007

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tom 29

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nr 1 Suppl.

11

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Effect of polymer supersorbent added to medium on the content of mineral elements in strawberry leaves and fruit

84%

Mikiciuk G., Mikiciuk M.

Journal of Elementology

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2010

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tom 15

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nr 2

313-319

The aim of the study was to evaluate the influence of polymer supersorbent AgroHydrogel, added to soil, on the content of some mineral elements in leaves and fruit of strawberry cv. Elsanta. In 2007-2008, a pot experiment set up in a system of complete randomization was carried out in four replications in a greenhouse of the West Pomeranian University of Technology in Szczecin. The experimental factor was the addition of two doses of AgoHydrogel to the medium. The two rates were 1.8 and 3.6 g dm–3, i.e. 15 and 30 g per a Kick’s pot versus the control which was the medium with no gel. The experiment was carried out indoors. Soil moisture was measured by means of contact soil tensometers. The plants were watered with 0.5 dm3 per pot and the tensometer, fitted in the medium with 15 g of gel per pot, showed 450 hPa. The content of the elements in strawberry leaves and fruit was determined by means of the AAS method. The applied polymer supersorbent decreased the cummulation of zinc and lead in leaves and copper, nickel and lead in fruit of strawberry. This effect particularly visible in plants growing in the medium with an increased dose of hydrogel. However, the application of AgroHydrogel did not affect the content of iron, manganese, copper and nickel in leaves and manganese, zinc and iron in fruit of the analyzed variety of strawberry.

12

Biotransformation of phosphogypsum in media containing different forms of nitrogen

84%

Rzeczycka M., Mycielski R., Kowalski W., Galazka M.

Acta Microbiologica Polonica

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2001

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tom 50

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nr 3-4

281-289

13

Effect of temperature and sucrose/nitrogen level in medium on growth and development of Clematis cultured in vitro

84%

Gabryszewska E., Kawa-Miszczak L., Wegrzynowicz-Lesiak E., Saniewski M.

Acta Physiologiae Plantarum

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2007

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tom 29

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nr 1 Suppl.

14

Lignite as a new medium in soilless cultivation of tomato

84%

Dysko J., Kaniszewski S., Kowalczyk W.

Journal of Elementology

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2015

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tom 20

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nr 3

Thus far, lignite has not been used as a growing medium in soilless cultivation. The aim of the research conducted in 2011-2013 was to develop a new growing medium made of lignite, and to determine the suitability of such a medium to prolonged soilless cultivation of tomato under greenhouse conditions. Lumps of lignite were crashed and sifted to obtain fractions of lignite particles 20, 10, 2.5 mm in diameter of and the earthy fraction. Cultivation mats measuring 100 x 20 x 7 cm were made of the particular fractions and a mixture of lignite fractions. These substrates were used in a prolonged cycle cultivation of the tomato cultivar Growdena, with the results compared to its cultivation in Grodan-Grotop rockwool. The crashing of lignite into small-grain fractions changed the substrate’s pH, salinity and concentration of S-SO4. Smaller lignite fractions resulted in the reduction of the medium’s pH and an increase of the salt content, particularly sulphate. Together with the crashing of lignite, the bulk density and water capacity increased, while the air capacity decreased. The most preferred air and water properties at pF=1.0 occurred in the medium with the fraction of lignite particles 2.5 mm in diameter and in the media formed by mixing different lignite fractions. The pH of the solution taken from the cultivation mats made of lignite was similar to the pH of the nutrient from rockwool. The concentration of nutrients in the lignite media throughout the whole cultivation period was similar to the concentration in rockwool. There was no biological sorption of nitrogen in the initial period of tomato cultivation on lignite and rockwool. The highest early, marketable and total yields of tomato fruits from cv Growdena F1 were obtained in the cultivation on mats made of the lignite fraction with particles 2.5 mm in diameter. These yields did not significantly differ from the marketable and total yields obtained in rockwool.

15

Production and quality appraisal of mycellium of parasol mushroom Macrolepiota procera [Scop. ex Fr.] Sing.

84%

Wozniak W.

Herba Polonica

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2009

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tom 55

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nr 3

285-291

The paper presents the production of mycelium of parasol mushroom Macrolepiota procera, an endangered mushroom species. Mycelium was produced in tissue culture, from which spawns were produced on five media. From the best production variant mycelium for consumption in highly processed foodstuffs was grown and analyzed chemically. Mycelium may also be used to produce mushrooms under natural conditions.

16

The effect of genotype and medium on plant regeneration from androgenic embryos

84%

Krzyzanowska D., Gorecka K., Kiszczak W., Kowalska U.

Journal of Fruit and Ornamental Plant Research

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2006

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tom 14

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nr Suppl.1

17

The influence of the mineral composition of the medium on in vitro propagation of Kohleria amabilis [Planch. et Linden] Fritsch shoots

84%

Kozak D., Hetman J., Witek M.

Acta Agrobotanica

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2007

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tom 60

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nr 1

The aim of the study was to test the influence of the mineral salt composition of the medium on in vitro multiplication and growth of kohleria shoots. Shoot tips were cultured on Murashige and Skoog (MS) (full- and half strength), Gamborg et al. (B₅), Nitsch and Nitsch (NN) or Lloyd and McCown (WPM) media supplemented with BA 1 mg·dm⁻³. The influence of passage time on the induction and growth of shoots on the medium containing half strength of MS mineral salts and BA 1 mg·dm⁻³, GA₃ 1 mg·dm⁻³ was also studied. The explants used in the experiments were obtained from aseptically grown shoot clusters. A significant influence of the medium type and the time of propagation on the number, length and weight of axillary shoots was observed. The medium containing full or half strength of mineral salts according to MS was the best. The propagation of kohleria shoots should take place at 4-week passages.

18

Micropropagation of asparagus [Asparagus officinalis L.] I. Multiplication in vitro

84%

Gorecka K., Krzyzanowska D., Gorecki R.

Vegetable Crops Research Bulletin

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2003

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tom 59

19

The influence of medium on viability of bovine morulae produced in vitro after their biopsy

84%

Lasiene K., Valanciute A., Lasys V., Vitkus A., Tusas S.

Polish Journal of Veterinary Sciences

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2006

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tom 09

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nr 3

The aim of this study was to determine the influence of two different media on the viability of in vitro produced biopsied bovine morulae. Bovine morulae were produced in vitro, then biopsied and cultured in the Ham's F10 and IVM media. Cultured and control morulae were stained with Hoechst 33342 and propidium iodide. Morulae were classified morphologically for excellent, good and degenerated quality. 42.86% of biopsied morulae cultured in the Ham's F10 medium and only 11.11% (only one) of these embryos cultured in the IVM were of excellent quality. Embryos of good quality were about 2 times less numerous in Ham's F10 medium (28.57%) than in IVM medium (55.5%) (P < 0.05). 28.57% of biopsied morulae cultured in Ham's F10 medium and 33.33% of these embryos cultured in the IVM degenerated (P > 0.05). The media had no significant influence on the number of total and viable blastomeres of morulae cultured in vitro after biopsy (P > 0.05). But the quantity of restored (excellent and good quality) embryos was higher when they were cultured after biopsy in Ham's F10 medium than in IVM. These statistically significant results (P < 0.05) show that the Ham's F10 medium is better for the restoring of biopsied bovine embryos produced in vitro than IVM.

20

Growth and activity of sulphate-reducing bacteria in media containing phosphogypsum and different sources of carbon

84%

Rzeczycka M., Blaszczyk M.

Polish Journal of Environmental Studies

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2005

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tom 14

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nr 6

The possibility of using a mixed community of sulphate-reducing bacteria (SRB) for the biotransformation of phosphogypsum was examined. The greatest reduction of phosphogypsum (g/l) was determined in cultures with lactate (3.3), ethanol or casein (2.7), almost two-fold less in media with glucose or lactose and threefold less in medium with acetate. In media with lactate or ethanol growth inhibition (I = μ (μ max) was slight (I = 0.80 or 0.79) but much higher in the case of cultures with lactose (I=0.41), glucose (I=0.54), acetate or casein (I=0.62). In those cultures in which the concentration of sulphides was very high (about 600 mg HS⁻/l) and the concentration of acetic acid did not exceed 10 mg/l (e. g. in cultures with glucose), inhibition of SRB was mainly caused by H₂S. In cultures with transient low pH value (e. g. containing lactose or acetate) the factor causing stronger growth inhibition was acetic acid. A condition for obtaining high SRB activity in media with phosphogypsum and fermentable carbon sources is constant monitoring of the reaction of the medium and/or counteracting the accumulation of toxic concentrations of hydrogen sulphide and acetic acid.

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