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A field experiment was carried out in 2008−2010 at the Agricultural Experimental Farm of the University of Natural Sciences and Humanities in Siedlce, located in Zawady. The experiment was established on light loamy soil which belonged to a very good rye complex. The experiment was designed in a two-factor split-plot pattern with three replicates. The factors examined in the experiment included three cultivars of edible potatoes: Cekin, Satina, Tajfun, and five weed control methods: application of the following herbicides (mixed or alone) Command 480 EC, Command 480 EC and Dispersive Afalon 450 SC, Stomp 400 SC, Stomp 400 SC and Dispersive Afalon 450 SC, while the control consisted of mechanical weeding without chemical protection. The aim was to determine the influence of selected herbicides and their combinations (Command 480 EC, Command 480 EC and Dispersive Afalon 450 SC, Stomp 400 SC, Stomp 400 SC and Dispersive Afalon 450 SC) on the content of copper, zinc and manganese in three potato cultivars. Potato tubers were harvested at full maturity. Samples of potato tubers for chemical analyses were taken from plots during harvest. Microelements in the dry matter of potato tubers were determined by the AAS method. The content of copper was significantly dependent on the cultivars, weed control methods and weather conditions during the growing season. In contrast, concentrations of depended on the cultivars and meteorological conditions, whereas the content of manganese in potato tubers was strongly conditioned by genetic traits of the cultivars.
A method for blood manganese measurements has been described. The method provides high reproducibility of the concentrations measured. The coefficients of variation were 3.4% and 4.1 % for within-run and between-runs, respectively. The recovery of the method was 98.1 %. Manganese concentrations in the blood ranged from 6.27 ng/ml to 8.98 ng/ml. The measurements revealed that contamination of tubes containing samples and prepared for determination can be the cause of the divergence of the results obtained.
Corn was grown for green matter in a pot experiment, in which soil was fertilized with composts alone or in combination with 3 g N per pot (urea). The composts were made in wooden boxes, measuring 50×60×60 cm. They were composted for 3.5 months, until the temperature in the composts became stable and equal to the ambient temperature. Rates of the composts were balanced with amounts of added nitroge, such as 6.0 g N per pot. A one-factor experiment was conducted in Kick-Brauckmann pots, kept in a greenhouse at the University of Warmia and Mazury in Olsztyn. After harvest, fresh matter of stems and leaves as well as corn cobs was determined. Next, the plant samples were dried to determine the dry matter content and finally, after mineralisation, the concentration of Cu, Zn and Mn was determined by atomic absorption spectrophotometry. The composts significantly reduced the concentration of zinc and manganese in the vegetative yield of cor (lstems and leaves). Urea applied in combination with the composts very strongly increased the concentration of manganese and, to a lesser degree, the level of zinc and copper in vegetative organs. In corn cobs, the application of urea increased only the content of manganese. Urea had a stronger effect on increasing the weight of cobs rather than the vegetative mass of corn plants. The uptake of Cu, Zn and Mn was more evidently conditioned by the accumulation of these elements in dry matter than by the total weight of corn plants. The extent of the relationship between the uptake of Cu, Zn and Mn and their concentration of corn biomass is expressed the by corresponding correlation coefficients: 0.66, 0.65 and 0.68.
The Premenstrual Syndrome (PMS) is described as a cyclical disorder related to the hormonal changes during a menstrual cycle, which affects the emotional and physical health of many women during their reproductive period of life. The PMS can obviously change the quality of life. The syndrome is characterized by a complex group of symptoms, such as depression, irritability, mood swings, anxiety, abdominal discomfort. These signs occur during the luteal phase of a menstrual cycle and disappear after the onset of menses. Some studies suggest that a variety of nutrients may play an important role in the mood swings which occur cyclically during the course of the premenstrual syndrome and that some can have a beneficial impact, especially on the estrous phase of a menstrual cycle. The aim of the paper is to review the results of some studies concerning the role of bioelements in patients with the PMS. Concentrations of magnesium, zinc, selenium and manganese are the highest during menses and the lowest in the ovulatory phase. Fluctuations of the magnesium concentration during a menstrual cycle and the involvement of this element in many cellular pathways and neuromuscular activities obviously affect the incidence or intensity of the PMS symptoms. However, we lack firm evidence that magnesium supplementation can have a positive effect on alleviating the the PMSrelated ailments. Some relationship between the PMS and bone demineralization or depressed calcium concentration in blood has been identified. However, further studies are necessary to examine how the calcium concentration in a human body can decrease the intensity of the PMS symptoms.
Statistical analysis of results indicated a significant positive correlation (r = 0.676, P<0.001) between manganese concentrations in serum and blood of cows. Manganese concentration measurements of 147 serum and 117 blood samples from cows in various stages of their production period and fed various manganese levels in their feed were used for determination of the range of reference values. The concentrations of manganese in lactating and drying cows ranged from 1.55 - 4.85 x ng/ml and 1.27 - 3.05 x ng/ml in the serum and from 5.10 ng/mL to 12.35 ng/ml and 4.40 ng/ml to 9.45 ng/ml in the blood, respectively. Analysis of the variance of mean manganese concentrations in the serum and blood revealed that the lowest concentrations of manganese were found in drying cows.
The objective of this study was to investigate the serum concentrations of iron, manganese, cobalt, copper, zinc, and copper/zinc ratio in Downer cows. The study was performed on 20 recumbent Downer cows and 20 healthy cows. The serum concentrations of iron (P=0.002) and copper (P=0.035) were lower in Downer cows compared with the healthy cows. Serum zinc concentrations, although not significantly (P=0.162), were also lower in Downer cows compared with the healthy cows. Cows with Downer syndrome had greater concentrations of serum AST (P<0.001), LDH (P=0.003), and CK (P<0.001). Other biochemical parameters remained unchanged. RBC (P=0.026), Hb (P=0.029), and MCV (P<0.001) in cows with Downer syndrome had lower values compared with those of healthy ones. However, WBC in cows with Downer syndrome had greater values (P<0.00l) compared with those of healthy ones. Other haematological parameters remained similar between Downer cows and healthy cows. It was concluded that serum iron and copper concentrations may play an important role for the aetiology and/or pathogenesis of Downer cows. These elements may be supplemented for the prevention and therapy of recumbency in cows.
Oxidative metabolism is essential for the gamete and the embryo energy production and is unavoidably associated with generation of reactive oxygen species (ROS). Enzymatic antioxidant defenses are present in the mammalian oocytes, embryos and follicular fluid (FF). An addition of porcine FF to maturation media have positive effects on the IVM and IVF results. The aim of this study was to study the CAT, SOD and GSH-Px activity, as well as Cu, Mn, and Zn concentration in porcine FF collected from the left and the right ovary. The ovaries were collected from 77 gilts at age 8 months. All the analyzed samples of FF revealed active enzymes (24.2·10–3 U · l–1, 2.65·10–3 U · l–1, and 525 U · l–1 for CAT, GSH-Px, and SOD, respectively) and contained Zn, Cu and Mn (13.8·103mol · l–1, 33.3·103 mol · l–1, and 133·10–9 mol · l–1, respectively). In the pFF collected from the left ovary, SOD and GSH-Px activity was higher compared to pFF from the right ovary. On the contrary, the concentration of Cu and Mn was significantly lower in the left ovary pFF. The concentration of Cu ions was negatively correlated with SOD activity. The CAT activity in pFF form left or right ovary did not show any differences.
In order to investigate the effect of zinc, iron and manganese fertilization on Zn, Fe and Mn concentrations in the stem and leaves and on the chlorophyll content in leaves of soybean at different reproductive growth stages, two experiments were conducted on a research field of the Islamic Azad University of Kermanshah, Iran, during the 2010 and 2011 growing seasons. The experimental design consisted of a factorial experiment based on the randomized complete block method with three replicates. The treatments included three levels of Zn (0, 20 and 40 kg ha-1), Fe (0, 25 and 50 kg ha-1), and Mn (0, 20 and 40 kg ha-1), all applied to soil. At 30, 60, 90 and 120 days after sowing (DAS), the SPAD readings were done on five leaves from each experimental plot for all replicates. Five plants were randomly selected from each plot at the flowering, pod setting, seed filling and maturity stages. Samples washed with distilled water were dried, weighed and incinerated at 550°C. Finally, the Zn, Fe and Mn concentrations were determined by Atomic Absorption Spectrometry. The results indicated that zinc application had significantly affected Zn, Fe and Mn concentrations in soybean stems at all the sampling times except for the soybean stem Mn concentration at the maturity stage. In contrast, the concentration of Mn at seed filling was unaffected by iron fertilization. Also, the maximum Fe concentration in soybean stems resulting from zinc application was achieved in Zn20 treatment. The highest Zn and Mn concentrations in leaves were recorded when iron was applied in smaller amounts. Furthermore, zinc, iron and manganese applications had significant effects on the leaf chlorophyll concentration during all the growth stages of soybean plants. In addition, increased iron and manganese fertilization raised the soybean leaf chlorophyll concentrations in all the samples. The maximum chlorophyll concentration in soybean leaves at 60 DAS was recorded in Zn20Fe50, Zn20Mn40 and Fe50Mn40 treatments (34.7, 34.0 and 35.2, respectively).
U 100 kobiet, które urodziły dzieci zdrowe (w terminie porodu) oznaczono metodą atomowej spektrometrii absorpcyjnej stężenie manganu w surowicy krwi żylnej i łożysku oraz przeprowadzono ankietą żywieniową. Pomiędzy stężeniem manganu w surowicy krwi a jego stężeniem w łożysku wykazano istotną korelację dodatnią. Częste spożycie pieczywa razowego wpływało na wzrost poziomu manganu w surowicy i łożysku. Zawartość tego pierwiastka w łożysku podwyższało także częste spożycie podrobów oraz picie soków i napojów owocowych.
Przedmiotem badań było określenie zawartości wybranych metali w tkance mięśniowej, wątrobie i nerkach koźląt i tryczków pochodzących z regionu Podlasia. Materiał badawczy stanowiły koziołki rasy białej uszlachetnionej i tryczki rasy polskiej owcy nizinnej. Średnie wartości stężenia magnezu w analizowanej tkance mięśniowej okazały się statystycznie «istotne między badanymi gatunkami zwierząt. Podobne zależności wykazano w wątrobie i nerkach. Ponadto istotnie wyższe wartości stężenia miedzi stwierdzono w wątrobie badanych koźląt (92,58 mg/kg). Mięso koziołków zawierało więcej Pb i mniej Cd w porównaniu z mięsem tryczków. Uzyskane w badaniach własnych zawartości: Cu, Mn, Fe, Zn w ocenianym mięsie mieściły się w granicach uznanych za fizjologiczne i nie stanowiły zagrożeń toksykologicznych.
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