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The dose-response curves for IAA and 4-Cl-IAA-induced growth of Zea mays L. coleoptile segments were studied as a function of time. Moreover, some characteristic growth parameters for both auxins were compared. The dose-response curve of growth rate measured after IAA or 4-Cl-IAA application was bell-shaped in all experiments. The optimum concentration was 10⁻⁶ M for 4-Cl-IAA and was found not to depend on the time of the growth measurement. However, in the case of IAA the optimum shifted from 10⁻⁶ M at the time of maximal growth rate to 10⁻⁵ M or even 10⁻⁴ M, when growth measured 3–4 hours after auxin application was analysed. The relative activity of 4-Cl-IAA-induced growth rate (as compared to IAA) increased significantly with increasing time from addition of this auxin to the medium. For both auxins the time needed to reach the maximal growth rate was clearly related to their concentrations. These data provided further evidence that 4-Cl-IAA is much more active auxin than IAA and can also suggest that IAA is more rapidly metabolized in comparison to 4-Cl-IAA.
Experiment with silage maize was established in 2002. Two hybrids were compared: early hybrid Birko (FAO 210) and middle early hybrid Etendard (FAO 260). Sum of effective temperatures was observed during vegetation period. There was confirmed significant dependence of sum of effective temperatures on dry matter production dynamic of silage maize. Based on study of this indicator is possible to assign optimal term of harvesting for specific hybrid on specific stand. Significant difference of dry matter content of variously matured hybrids is reached at the end of vegetation period.
The system consisting of a few proportional detectors with appropriate electronic components was earlier developed for in vivo studies of long distance transport in whole maize seedlings. ¹⁴CO₂ assimilation rate (Pa), time of radioactivity appearing in the loading region (AT), transport speed in the leaf (TSl), transport speed between the leaf and the roots (TSr), the maximum radioactivity values detected in the leaf below the feeding area (Rl) and in the mesocotyl (Rr) from leaves to roots in maize seedlings were calculated from the obtained temporal profiles of radioactivity. The study was undertaken to follow the changes in separate steps of long distance transport in maize seedlings as affected by two light irradiances and application of p-chloromercuribenzenesulphonic acid and fusicoccin, with the aim to investigate different steps of long distance transport, particularly phloem loading. The method used allows to study in vivo the different aspects of long distance transport in maize seedlings, both qualitatively and quantitatively. It was shown that the characteristics obtained from the radioactivity profiles corresponded to different steps of long distance transport, as assimilate synthesis, phloem loading, and phloem translocation. It was also demonstrated that although active phloem loading participate in assimilate export from the leaves, assimilate transport along the maize seedling might undergo accordingly to assimilate gradient, particularly under light irradiance higher than during the growth.
A protein kinase of 57 kDa, able to phosphorylate tyrosine in synthetic substrates pol(Glu4,Tyr1) and a fragment of Src tyrosine kinase, was isolated and partly puri­fied from maize seedlings (Zea mays). The protein kinase was able to phosphorylate exogenous proteins: enolase, caseins, histones and myelin basic protein. Amino acid analysis of phosphorylated casein and enolase, as well as of phosphorylated endoge­nous proteins, showed that both Tyr and Ser residues were phosphorylated. Phosphotyrosine was also immunodetected in the 57 kDa protein fraction. In the protein fraction there are present 57 kDa protein kinase and enolase. This co-purifi­cation suggests that enolase can be an endogenous substrate of the kinase. The two proteins could be resolved by two-dimensional electrophoresis. Specific inhibitors of typical protein-tyrosine kinases had essentially no effect on the activity of the maize enzyme. Staurosporine, a nonspecific inhibitor of protein kinases, effectively inhib­ited the 57 kDa protein kinase. Also, poly L-lysine and heparin inhibited tyrosine phosphorylation by 57 kDa maize protein kinase. The substrate and inhibitor specificities of the 57 kDa maize protein kinase phosphorylating tyrosine indicate that it is a novel plant dual-specificity protein kinase.
In growing maize, an increase in the content of phenolic compounds and selected phenolic acids in soil was found after the incorporation of white mustard, buckwheat, spring barley, oats and rye mulches into the soil. The highest content of phenolic compounds in soil was found after oats mulch incorporation (20% more than in the control soil). The highest content of selected phenolic acids was found for the soil with the oats and rye mulch. Among the phenolic acids investigated, ferulic acid was most commonly found in the soil with the plant mulches. However, two phenolic acids: the protocatechuic and chlorogenic acid, were not detected in any soil samples (neither in the control soil nor in the mulched soil). At the same time, a decrease in the primary weed infestation level in maize was found in the plots with all the applied plant mulches, especially on the plots with oats, barley and mustard. The plant mulches were more inhibitory against monocotyledonous weeds than dicotyledonous ones. During high precipitation events and wet weather, a rapid decrease in the content of phenolic compounds in soil and an increase in the primary weed infestation level in maize were observed.
The genetic basis of different traits and the effect of chromosomal regions on the expression of such traits is the primary interest of this study. In the present investigation, the effect of chromosomal regions on yield and its two primary components was studied, and two genetic parameters, additive effect and degree of dominance, were estimated based on a single-locus model using markers with significant effects on the traits (informative markers). Eight inbred lines from diverse geographical regions of India were crossed in all possible pairs, and F1S were evaluated for the yield and yield components in a replicated trial in two environments. Sequential path analysis was employed to find those yield components showing significant direct effects on yield with negligible multicollinearity. The parental lines were profiled using 56 polymorphic SSR (Simple Sequence Repeat) markers covering 10 chromosomes of maize. Stepwise multiple regression analysis was used to determine the informative markers on yield and its primary components detected through path analysis. Fourteen markers were found to have association with chromosomal regions showing significant effects on the total grain yield, 100-grain weight and total number of kernels per ear. Chromosome 1 with four informative markers revealed the highest genic effects on yield and its components. Markers bnlg594 and bnlg1360 on chromosome 10, and bnlg147 on chromosome 1 revealed the highest additive effects on the total grain yield, 100-grain weight and total number of kernels per ear, respectively. For the analyzed traits, overdominance occurred in all the loci and d/a values, varying from 8.60 for 100-grain weight to 1.40 for total grain yield.
Eighty maize grain samples collected in Nigeria were investigated for fumonisin B1 (FB1) content and Fusarium verticillioides colonization. F. verticillioides DNA was quantified by species-specific real-time PCR and living propagules of the fungus were counted by agar-plating method. FB1 was detected in 55 (68.7%) of the total samples (mean: 98.5 μg/kg, range: 10 to 714 μg/kg) at 10 μg/kg detection limit. The mean amount of F. verticillioides DNA determined by real-time PCR was 49.7 μg/kg (range: 10-126.7 μg/kg), while agar plate method showed the presence of F. verticillioides in 45 samples (mean incidence: 21.0%, range: 6.7-60.0%). There was correlation ties between F. verticillioides DNA by real time PCR and fungal colonization by agar plate method (R = 0.71, p = 00001 at 95% confidence level), and means of FB1 and F. verticillioides DNA in the yellow and white maize were significantly different. Despite the high consumption of maize in Nigeria, the amount of FB1 ingested by consumers appears to be low. The estimated daily intake of fumonisins was 0.21 μg/kg body weight per day.
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