Contents of ginkgoflavonoglycosides and macronutrients were analysed in leaves of maidenhair trees, collected from one- and two-year-old shoots, at four dates in 2002, from a field plantation established from a selected biotype. Gingkoflavonoglycoside contents were always higher in leaves collected from one-year-old shoots and ranged from 1.28% (30 August) to 1.54% (15 July). The highest contents of nitrogen, calcium and magnesium were recorded in leaves from one-year-old shoots, while that of potassium was higher in leaves collected from one-year-old shoots. Phosphorus content in July and mid-August was similar in one- and two-year-old leaves. In the third decade of August, especially in leaves collected from one-year-old shoots, phosphorus content was the lowest, amounting to 0.20%.