Wyniki wyszukiwania

1

CD30 expression on allergen- and non-allergen-specific T cell lines and its role in cytokine production

100%

Tarkowski M.

Archivum Immunologiae et Therapiae Experimentalis

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2003

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tom 51

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nr 5

2

Correlation between porcine PPARGC1A mRNA expression and its downstream target genes in backfat and longissimus dorsi muscle

86%

Erkens T., Vandesompele J., Van Zeveren A., Peelman L. J.

Journal of Applied Genetics

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2009

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tom 50

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nr 4

361-369

Knowledge of in vivo relationship between the coactivator PPARGC1A and its target genes is very limited, especially in the pig. In this study, a real-time PCR experiment was performed on longissimus dorsi muscle (MLD) and backfat with 10 presumed PPARGC1A downstream target genes, involved in energy and fat metabolism, to identify possible relationships with PPARGC1A mRNA expression in vivo in the pig (n = 20). Except for UCP3 and LPL, a very significant difference in expression was found between MLD and backfat for all genes (P < 0.01). Hierarchical cluster analysis and the significant pairing of mRNA expression data between sampling locations suggested a genetic regulation of the expression of several target genes. A positive correlation with PPARGC1A was found for CPT1B, GLUT4, PDK4, and TFAM(P < 0.0001). A negative correlation was found for UCP2, FABP4, LEP (P < 0.0001), and TNF (P = 0.0071). No significant correlation was detected for UCP3 and LPL. This study provides evidence for a clear difference in mRNA expression of crucial genes in fat and energy metabolism between 2 important tissues. Our data suggest a clear impact of PPARGC1A on energy and lipid metabolism in vivo in the pig, through several of these downstream target genes.

3

Pharmacological characterization of lipidized analogs of prolactin-releasing peptide with a modified c-terminal aromatic ring

86%

Prazienkova V., Ticha A., Blechova M., Spolcova A., Zelezna B., Maletinska L.

Journal of Physiology and Pharmacology

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2016

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tom 67

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nr 1

4

Altered dopamine signalling in chronic epigastric pain syndrome

72%

Chojnacki C., Poplawski T., Blasiak J., Fila M., Konrad P., Chojnacki J.

Journal of Physiology and Pharmacology

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2020

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tom 71

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nr 6

5

Immunolocalization of leptin receptor and mRNA expression of leptin and estrogen receptors as well as caspases in the chorioallantoic membrane (CAM) of the chicken embryo

72%

Grzegorzewska A. K., Lis W., Sechman A.

Folia Biologica

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2016

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tom 64

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nr 2

6

Adropin suppresses insulin expression and secretion in INS-1E cells and rat pancreatic islets

72%

Billert M., Jasaszwili M., Strowski M., Nowak K. W., Skrzypski M.

Journal of Physiology and Pharmacology

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2020

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tom 71

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nr 1

7

In vitro effects of TCDD, PCB126 and PCB153 on estrogen receptors, caspases and metalloproteinase-2 mRNA expression in the chicken shell gland

72%

Hrabia A., Lesniak A., Sechman A.

Folia Biologica

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2013

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tom 61

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nr 3-4

8

Arecoline inhibits interleukin-2 secretion in Jurkat cells by decreasing the expression of alpha7-nicotinic acetylcholine receptors and prostaglandin E2

72%

Hwang G. S., Hu S., Lin Y. H., Chen S. T., Tang T. K., Wang P. S., Wang S. W.

Journal of Physiology and Pharmacology

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2013

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tom 64

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nr 5

9

Expression of alpha and beta estrogen receptors in the chicken ovary

72%

Hrabia A., Wilk M., Rzasa J.

Folia Biologica

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2008

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tom 56

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nr 3-4

187-191

10

Adaptation of myosin heavy chain mRNA expression after implantation of polyhydroxybutyrate scaffolds in rat m. latissimus dorsi

72%

Mack H. B., Mai R., Lauer G., Mack F., Gedrange T., Franke R., Gredes T.

Journal of Physiology and Pharmacology. Supplement

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2008

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tom 59

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nr 5

95-103

11

Zolpidem withdrawal induced uncoupling of GABAA receptors in vitro associated with altered GABAA receptor subunit mRNA expression

72%

Jazvinscak Jembrek M., Vlainic J., Suran J.

Acta Neurobiologiae Experimentalis

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2015

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tom 75

|

nr 2

Hypnotic Zolpidem produces its effects via the benzodiazepine binding site in a1-containing GABAA receptors. The aim of the study was to assess the influence of duration of Zolpidem treatment and its withdrawal, as well as the role of a1-containing GABAA receptors in the development of physical dependence and tolerance. Namely, recombinant receptors can be used to characterize mechanisms involved in different processes in the brain and to delineate the contribution of specific receptor subtypes. To address the influence of chronic Zolpidem treatment we exposed HEK293 cells stably expressing a102y2S recombinant GABAA receptors for seven consecutive days, while withdrawal periods lasted for 24, 48, 72 and 96 hours. Using radioligand binding studies we determined that chronic Zolpidem treatment did not induce changes in either GABAA receptor number or in the expression of subunit mRNAs. We observed the enhancement of binding sites and upregulated expression of subunit mRNAs only following 96-hour withdrawal. Moreover, Zolpidem treatment and its withdrawal (all time points) induced functional uncoupling between GABA and benzodiazepine binding sites in the GABAA receptor complex. Accordingly, it might be assumed that Zolpidem withdrawal-induced uncoupling of GABAA receptors is associated with altered GABAA receptor subunit mRNA expression. The results presented here provide an insight into molecular and cellular mechanisms probably underlying adaptive changes of GABAA receptor function in response to chronic usage and withdrawal of zolpidem and perhaps the observed molecular changes could be linked to the tolerance and dependence produced upon prolonged treatment with other GABAergic drugs.

12

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Gonadotropin-releasing hormone and kisspeptin-10 regulate nuclear receptor subfamily 5 group a member 1/catenin beta 1/nuclear receptor subfamily 0 group B member 1 activity in female rat anterior pituitary gland

72%

Zielinska-Gorska M., Gorski K., Biernacka K., Sawosz E., Kaminski T., Gajewska A.

Journal of Physiology and Pharmacology

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2018

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tom 69

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nr 3

13

Analysis of integrins and vascular endothelial growth factor isoforms mRNA expression in the canine uterus during perimplantation period

72%

Bukowska D., Kempisty B., Jackowska M., Wozna M., Antosik P., Piotrowska H., Jaskowski J. M.

Polish Journal of Veterinary Sciences

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2011

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tom 14

|

nr 2

Integrins are the major receptors within the extracellular matrix (ECM) that mediate several functions connected with cell life and metabolism, such as cell adhesion, migration, cytoskeletal organization, proliferation, survival, and differentiation. A vascular endothelial growth factor (VEGF) is one of the most important angiogenic factors. It has been suggested that the expression of this gene may play crucial physiological roles in reproductive organs. All investigated endometrial tissues were isolated on day 10-12 after mating. Control bitches, used in this study, were in metestrus, which was determined according to the vaginal cytology and progesterone level in blood. Early pregnancy was verified by flushing the uterine horns with PBS. Total RNA was isolated from the bitches endometrium by means of the Chomczyński and Sacchi method, treated by DNase I, and reverse-transcribed into cDNA. A quantitative analysis of integrins α2b, β2 and β3, VEGF 164, 182 and 188 cDNA was performed by RT-PCR. In results we have shown an increased expression of all investigated genes (integrins α2b, β2 and β3, VEGF 164, 182, and 188) in pregnant bitches uterus as compared to non-pregnant females (P<0.001). Our results indicated that the expression of genes encoding integrins and vascular endothelial growth factors is different in relation to the time of the embryo implantation and it is increased in the first period of this process. This may be associated with the induction of specific mechanisms responsible for receptivity of uterus following the embryo attachment. In addition, all of investigated genes are up-regulated in a pregnancy-specific manner and the increased expression of these genes may regulate the uterus function during the implantation of canine embryos.

14

Experimental autoimmune myocarditis in rats and therapeutic histamine H1 – H4 receptor inhibition

72%

Stasiak A., Gola J., Kraszewska K., Mussur M., Kobos J., Mazurek U., Stark H., Fogel W. A.

Journal of Physiology and Pharmacology

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2018

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tom 69

|

nr 6

15

Mesenteric artery reactivity and small intestine morphology in a chicken model of hypoxia-induced fetal growth restriction

72%

Moonen R. M. J., Kessels C. G. A., Zimmermann L. J. I., Villamor E.

Journal of Physiology and Pharmacology

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2012

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tom 63

|

nr 6

16

Changes in growth hormone secretion and leptin receptor mRNA expression under the influence of leptin and adrenocorticotropin in pituitary cells of early weaned ewe lambs

72%

Kosior-Korzecka U., Wojcik M., Longo V., Puzio I., Nowakiewicz A., Patkowski K., Gregula-Kania M.

Journal of Physiology and Pharmacology

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2019

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tom 70

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nr 4

17

mRNA expression and immunocytochemical localization of leptin receptor in the oviduct of the laying hen [Gallus domesticus]

72%

Grzegorzewska A. K., Paczoska-Eliasiewicz H. E., Rzasa J.

Folia Biologica

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2008

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tom 56

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nr 3-4

179-185

18

Puerperal influence of bovine uterine health status on the mRNA expression of pro-inflammatory factors

72%

Peter S., Michel G., Hahn A., Ibrahim M., Lubke-Becker A., Jung M., Einspanier R., Gabler C.

Journal of Physiology and Pharmacology

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2015

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tom 66

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nr 3

19

Quantification of thioredoxin mRNA expression in the rat hippocampus by real-time PCR following oxidative stress

72%

Yalcin A.

Acta Biochimica Polonica

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2004

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tom 51

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nr 4

Thioredoxin (Trx) is a multifunctional protein with a redox-active disulfide/dithiol in the active site. Thioredoxin, with its redox-regulating and reactive oxygen species (ROS) scavenging activities, plays several important biologic roles both in intracellular and extracellular compartments. The purpose of this report was to quantify the relative expression of Trx in rat hippocampus following an oxidative stress-involving treatment such as kainic acid (KA) using real-time PCR and the 2-AACt method. The relative changes in expression of Trx mRNA in KA-treated and control animals were significantly different as 2.02 ± 0.77 and 1.0 ± 0.26, respectively (P < 0.05). Minimum and maximum n-fold changes in Trx expression in KA-treated and control animals were determined as (1.4-5.2) and (0.8-1.3), respectively. Thus, real-time PCR and the 2-AACt method for data analysis from real-time PCR were found to be an accurate and sensitive method for quantifying Trx mRNA levels.

20

The effects of indomethacin on angiogenic factors mRNA expression in renal cortex of healthy rats

72%

Mucha K., Foroncewicz B., Koziak K., Czarkowska-Paczek B., Paczek L.

Journal of Physiology and Pharmacology

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2007

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tom 58

|

nr 1

Indomethacin is a nonsteroidal anti-inflammatory drug used frequently to control chronic or temporary pain. In the kidney, indomethacin decreases medullary and cortical perfusion, resulting in hypoxia. Kidney hypoxia has many effects, including changes in gene expression, and is a strong stimulus for angiogenesis. Other angiogenic factors include vascular endothelial growth factor (VEGF), basic fibroblast growth factor (FGF-2), transforming growth factor beta 1 (TGFß1), and platelet-derived growth factor (PDGF). Our goal was to examine the influence of indomethacin on mRNA expression of these factors and their selected receptors in the renal cortex of healthy rats. Groups of 8 healthy, male, six-week-old Wistar rats received either indomethacin (5 mg/kg/day) or placebo orally for three months. RNA from renal cortex biopsies was analyzed by real-time polymerase chain reaction to quantify the mRNA levels of each cytokine. We observed significantly higher mRNA levels for VEGF (1.73-fold), FGF-2 (5.6-fold) and TGFß receptor III (2.93-fold), PDGF receptor alpha (2.93-fold) and receptor ß (2.91-fold) in rats receiving indomethacin compared to rats given placebo (p < 0.05). Amounts of mRNA for TGFß1, PDGF, FGF receptors 1 and 2 and TGFß receptor I did not differ between analysed groups. Our data indicates that indomethacin may regulate the expression of potent angiogenic factors VEGF and FGF-2.

Ograniczanie wyników

CD30 expression on allergen- and non-allergen-specific T cell lines and its role in cytokine production

Correlation between porcine PPARGC1A mRNA expression and its downstream target genes in backfat and longissimus dorsi muscle

Pharmacological characterization of lipidized analogs of prolactin-releasing peptide with a modified c-terminal aromatic ring

Altered dopamine signalling in chronic epigastric pain syndrome

Immunolocalization of leptin receptor and mRNA expression of leptin and estrogen receptors as well as caspases in the chorioallantoic membrane (CAM) of the chicken embryo

Adropin suppresses insulin expression and secretion in INS-1E cells and rat pancreatic islets

In vitro effects of TCDD, PCB126 and PCB153 on estrogen receptors, caspases and metalloproteinase-2 mRNA expression in the chicken shell gland

Arecoline inhibits interleukin-2 secretion in Jurkat cells by decreasing the expression of alpha7-nicotinic acetylcholine receptors and prostaglandin E2

Expression of alpha and beta estrogen receptors in the chicken ovary

Adaptation of myosin heavy chain mRNA expression after implantation of polyhydroxybutyrate scaffolds in rat m. latissimus dorsi

Zolpidem withdrawal induced uncoupling of GABAA receptors in vitro associated with altered GABAA receptor subunit mRNA expression

Gonadotropin-releasing hormone and kisspeptin-10 regulate nuclear receptor subfamily 5 group a member 1/catenin beta 1/nuclear receptor subfamily 0 group B member 1 activity in female rat anterior pituitary gland

Analysis of integrins and vascular endothelial growth factor isoforms mRNA expression in the canine uterus during perimplantation period