Wyniki wyszukiwania

1

Production of prostacyclin and prostaglandin E2 in resting and IL-1beta-stimulated A549, HUVEC and hybrid EA.HY 926 cells

100%

Olszanecki R., Gebska A., Korbut R.

Journal of Physiology and Pharmacology

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2006

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tom 57

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nr 4

Production of arachidonic acid (AA) metabolites - prostacyclin (PGI2) in large vessels and prostaglandin E2 (PGE2) in microcirculation is intrinsically involved in maintenance of vascular wall homeostasis. EA.hy 926 is a hybrid cell line, is derived by fusion of HUVEC with A549 cells. The aim of this study was to examine the production of prostacyclin and PGE2 in resting and IL-1ß-stimulated EA.ha 926 cells, in comparison with its progenitor cells. Non-stimulated EA.hy 926 cells has been found to produce much lower amounts of prostacyclin than resting HUVEC. Resting hybrid cells produced more PGE2 than prostacyclin, despite they expressed high levels of COX-1 and PGI2 synthase. On the contrary to HUVEC and A549, EA.hy 926 cells did not respond to IL-1ß with COX-2 induction and increase of prostaglandin production, however they did it in response to lysophosphatidylcholine (LPC). The characteristics of EA.hy 926 cells in terms of the pattern of prostanoid formation could facilitate studies on endothelial metabolism and role of these important lipid mediators.

2

Lysophosphatidylcholine triggers cell differentiation in the protozoan parasite Herpetomonas samuelpessoai through the CK2 pathway

84%

Dutra F. L., Vieira D. P., Coelho F. S., Adade C. M., Atella G. C., Silva Neto M. A. C., Lopes A. H.

Acta Parasitologica

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2020

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tom 65

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nr 1

3

Defining sex differences in selected lipid metabolites of blood plasma in Wistar rats

84%

Leskanicova A., Chovancova O., Babincak M., Blicharova A., Kolesarova M., Macekova D., Kostolny J., Smajda B., Kiskova T.

Journal of Physiology and Pharmacology

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2019

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tom 70

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nr 4

4

Lysophosphatidylcholines: bioactive lipids generated during storage of blood components

84%

Maslanka K., Smolenska-Sym G., Michur H., Wrobel A., Lachert E., Brojer E.

Archivum Immunologiae et Therapiae Experimentalis

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2012

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tom 60

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nr 1

5

Superoxide is a potential culprit of caspase-3 dependent endothelial cell death induced by lysophosphatidylcholine

67%

Park S., Kim J. A., Choi S., Suh S. A.

Journal of Physiology and Pharmacology

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2010

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tom 61

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nr 4

375-381

Oxidative stress in the vascular wall has intimately been implicated in the apoptosis of human umbilical vein endothelial cells (HUVECs) by lysophosphatidylcholine (LPC). However, the major type of reactive oxygen species (ROS) in this apoptotic signaling pathway remains to be clarified. In this study, we report that superoxide mediate LPC-induced caspase-3 dependent apoptosis in cultured HUVECs. The stimulation of HUVECs with LPC evoked apoptosis and ROS generation, and inhibited nitric oxide (NO) production in a dose-dependent manner. The classical caspase-3 dependent apoptosis was determined after 16 hours treatment by Western blotting using an antibody against cleaved caspase-3. The caspase-3 activation induced by LPC was prominently inhibited by antioxidants or NO donors and enhanced by NO inhibitors. Especially, LPC-induced caspase-3 activation was inhibited by superoxide dismutase (SOD) and enhanced by ammonium tetrathiomolybdate, SOD inhibitor. Additionally, xanthine/xanthine oxidase mixture increased the caspase-3 activation but catalase failed to reduce this superoxide-induced caspase-3 activation. These findings indicate that the superoxide generation caused by LPC activates the caspase-3 which results in HUVECs death. This study reveals some evidences linking superoxide with caspase-3 activation and provides a new dimension to superoxide-mediated caspase-3 activation in developing the endothelial dysfunction and atherosclerosis.

Ograniczanie wyników

Production of prostacyclin and prostaglandin E2 in resting and IL-1beta-stimulated A549, HUVEC and hybrid EA.HY 926 cells

Lysophosphatidylcholine triggers cell differentiation in the protozoan parasite Herpetomonas samuelpessoai through the CK2 pathway

Defining sex differences in selected lipid metabolites of blood plasma in Wistar rats

Lysophosphatidylcholines: bioactive lipids generated during storage of blood components

Superoxide is a potential culprit of caspase-3 dependent endothelial cell death induced by lysophosphatidylcholine