Wyniki wyszukiwania

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Life quality of patients with allergy

100%

Jedrzejewska B., Cimek I., Kowalska M.

Polish Journal of Environmental Studies

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2004

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tom 13

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nr Suppl.2, Part 1

Allergic diseases are categorized as civilization diseases. The highest rise of sick count is reported in highly developed countries. The course of these illnesses is very long and require long-term therapy, and a high level of patients dedication. They pose a great threat of medical and social nature. The aim of the study was to determine how allergic diseases influence the quality of life of the patients. The study was carried out amongst 110 people with allergy-based diseases. The tool used was the standard interview questionnaire. Amongst the group, 58.2% were women, and 64.5% lived in a city, in 57.3% of the cases allergy has occurred after reaching the age of 18. 59.1% required constant therapy. Because of the disease 35.5% of patients had to change the family's diet. 27.3% had to remove all animals, 15.5% had to remove house plants, 57.7% changed the way of doing laundry and cleaning. Because of the disease, 33.6% had to limit contact with other people.

2

A microarray gene analysis of peripheral whole blood in normal adult male rats after long-term GH gene therapy

84%

Qin Y., Tian Y.-P.

Cellular and Molecular Biology Letters

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2010

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tom 15

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nr 2

The main aims of this study were to determine the effects of GH gene abuse/misuse in normal animals and to discover genes that could be used as candidate biomarkers for the detection of GH gene therapy abuse/misuse in humans. We determined the global gene expression profile of peripheral whole blood from normal adult male rats after long-term GH gene therapy using CapitalBio 27 K Rat Genome Oligo Arrays. Sixty one genes were found to be differentially expressed in GH gene-treated rats 24 weeks after receiving GH gene therapy, at a two-fold higher or lower level compared to the empty vector group (p < 0.05). These genes were mainly associated with angiogenesis, oncogenesis, apoptosis, immune networks, signaling pathways, general metabolism, type I diabetes mellitus, carbon fixation, cell adhesion molecules, and cytokine-cytokine receptor interaction. The results imply that exogenous GH gene expression in normal subjects is likely to induce cellular changes in the metabolism, signal pathways and immunity. A real-time qRT-PCR analysis of a selection of the genes confirmed the microarray data. Eight differently expressed genes were selected as candidate biomarkers from among these 61 genes. These 8 showed five-fold higher or lower expression levels after the GH gene transduction (p < 0.05). They were then validated in real-time PCR experiments using 15 single-treated blood samples and 10 control blood samples. In summary, we detected the gene expression profiles of rat peripheral whole blood after long-term GH gene therapy and screened eight genes as candidate biomarkers based on the microarray data. This will contribute to an increased mechanistic understanding of the effects of chronic GH gene therapy abuse/misuse in normal subjects.

3

Treatment of Churg-Strauss syndrome with an inhaled corticosteroid after oral steroids discontinuation due to side effects

67%

Swietlik E., Doboszynska A.

Journal of Physiology and Pharmacology. Supplement

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2008

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tom 59

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nr 6

689-695

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Journal of Physiology and Pharmacology. Supplement

1 Polish Journal of Environmental Studies

1 Cimek I.

1 Doboszynska A.

1 Jedrzejewska B.

1 Kowalska M.

1 Qin Y.

1 Swietlik E.

1 Tian Y.-P.

1 2010

1 2008

1 2004

Life quality of patients with allergy

A microarray gene analysis of peripheral whole blood in normal adult male rats after long-term GH gene therapy

Treatment of Churg-Strauss syndrome with an inhaled corticosteroid after oral steroids discontinuation due to side effects