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A multiple linear regression analyses were performed to screen for the significant factors simultaneously influencing production of delta-endotoxin, proteolytic activities and spore formation by a Bacillus thuringiensis kurstaki strain. Investigated factors included: pH of the medium, available oxygen and inoculum size. It was observed that oxygen availability was the most influencing setting on both deltaendotoxins production and spores counts, followed by initial pH of the medium and inoculum size. On other hand, pH of medium was found to be the most significant parameter for proteolytic activity, followed by inoculum size and dissolved oxygen. Our results suggested that the first order with two-factor interaction model seemed to be more satisfactory than simple first order model for optimization of delta-endotoxin overproduction. The coefficients of determination (R²) indicated a better adequacy of the second order models to justify the obtained data. Based on results, relationships between delta-endotoxins production, proteolytic activities and spores counts were established. Our results can help to balance delta-endotoxins production and its stability.
Many studies on the changes in the rhythm of nature (animate and inanimate) revealed that plants are good indicators of climate change, because they are responsive to air temperature variations. There is a clear trend towards earlier onset of plant development in Poland, especially for spring phenological events as flowering and leafing. The main objective of this study was to identify phenological tendencies of selected plant indicator species in Poland and to explore the statistical relationship between mean phenological onset date against mean monthly air temperature. Phenological observations of 9 plant species: Corylus avellana L., Tussilago farfara L., Padus avium L., Taraxacum officinale F.H. Wiggers coll., Betula pendula Roth, Syringa vulgaris L., Aesculus hippocastanum L., Robinia pseudoacacia L., Tilia cordata Mill. from 25 locations acquired in the period 1951–1990 were used. The results proved that phenological changes were the reaction of plants to air temperature variations. For spring and summer phenophases, the mean correlation coefficient was negative — higher air temperature was related to earlier onset dates. The advance of spring and summer phenology was estimated at -2.2 days per 1°C. Majority of phenological events were the most significantly correlated with mean monthly air temperature of the preceding months. Phenological trends observed in Poland were mainly negative (73% of analysed stations), significant (22%) but diversified between the selected species. Linear regression analysis of the onset dates against year (including regression coefficient) showed the highest values for Corylus avellana flowering: -0.24 days per year (20% significant) and Betula pendula leaf unfolding: -0.18 days per year (33% significant). Robinia pseudoacacia showed significant advances (72% of negative trends were significant) in a summer season with an average rate of -0.3 days per year. Whereas in case of Tilia cordata no tendencies to advance has been proved.
The long-term evolution of the river beds of Nida River in Pińczów and Wiślica gauging station as well as an analysis of the causes of significant cases of lowering of the bottom of stream beds are presented in the paper. The intensity of river bed evolution as a stochastic process is described with the use of a statistical model. The analysis of the intensity of the process of river bed erosion was conducted under the assumption that the observed long-term trend of the lowest annual water levels reflect the elevation of the river bottom in the same years. The recorded observations at the Pińczów and Wiślica gauging stations on the Nida River, covering over 70-year period of time, are analyzed. Observations of the variability in the studied bottom levels over the decades allow concluding that the great transformation of riverbeds occurred there. The observations and calculations show that in Pińczów multistream or meandering river bed changed into straight channel and the process of shallowing of the river bed appeared. At the Wiślica gauging station the process of riverbed erosion with the variable intensity was observed. The research shows that the causes of these changes observed in Pińczów and Wiślica are not natural, but rather of human activity.
The work aimed at determination of the share of individual components in the yield of spring barley and field bean cultivated in 1993-1995. The method applied was linear regression using (or not) the logarithmic transformation. The study revealed that yields of spring barley and field bean were determined by a number of ears and plants per 1 m². However, the mass of 1000 grains had a small share in the yield formation. Individual share of yield components in yield of barley grain was more influenced by changes in its structural traits over the years, than the corresponding share in yield of field bean seeds. Full models were the best for predicting yields of both crops using linear regression. For barley the sufficient exactness was achieved also using models with two yield components, in which the number of ears per 1 m² had to be included. The above holds as well for field bean yield, proven that logarithmic transformation of the data has been previously done.
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