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The paper discusses the scattering of radiation by a Baltic crude oil –sea water emulsion. The scattering spectrum calculated using the Mie solution in the spectral range from 380 nm to 730 nm is compared with the measured spectrum of light scattered through a right angle. Spectra in the wavelength range from 210 nm to 730 nm were measured using a spectrofluorimeter for fresh and stored samples of the Baltic crude oil emulsion. Scattering increases with wavelength in the UV range and then decreases slightly with the wavelength of visible light. The result of the calculation is similar to the measured spectra. Both the calculated and measured spectra display numerous relative extremes throughout the spectral area. Light scattering in the emulsion decreases during storage as the oil concentration in the medium diminishes. The results also demonstrate that the single scattering model describes the phenomenon correctly.
Kinetics of the smooth muscle calponin-F-actin interaction was studied by stopped- flow measurements of light scattering and fluorescence intensity of pyrene-labelled F-actin. The intensity and character of the changes in light scattering, and thus the mode of calponin binding to actin filaments leading to changes in their shape and bun­dling, depend on the molar ratio of the two proteins. Parallel measurements of pyrene-fluorescence quenching upon calponin binding revealed that intrinsic conformational changes in actin filaments are delayed relative to the binding process and are not markedly influenced by the mode of calponin binding. Bundling of actin filaments by calponin was not correlated with fluorescence changes and thus with al­terations in the structure of actin filaments.
This paper presents the results of modeling of spectra of light scattering and absorption coefficients in sea water polluted with crude oil suspension. In the Mie theory, which has been applied for calculations, the results of the measurements of the size distribution spectra in artificial crude oil suspensions in sea water were used as well as the results of the measurements of the components of the complex light refraction index of crude oil from the Baltic Sea (Petrobaltic type). The comparison of the spectra obtained with those which are characteristic for the waters in the Gulf of Gda.sk confirmed that the admixture of emulsion with about 2 ppm of crude oil doubles the light scattering coefficient. The same effect, with respect to the light absorption coefficient, is obtained when the concentration of the crude oil suspension is about 100 ppm.
The paper analyses the optical properties of a crude oil –sea water emulsion, which is a form of petroleum pollution of the sea. These properties depend on the spillage concentration, the optical characteristics of the seawater and oil in question, and on the size distribution of the oil droplets. They may be described by the attenuation specific cross-sections and absorption specific cross-sections. Specific cross-sections and other optical parameters for droplets of a Baltic crude oil –Bal tic seawater emulsion were calculated using Mie’s solution. These characteristics were computed for fresh and weathered petroleum.
The paper presents some results of research on the fluorescence properties of petroleum oil-in-water emulsions. The research addresses the question of measuring and modelling light fields in an oil-polluted marine environment. The fluorescence of the emulsion manifests itself in the spectral range from 260 to over 400 nm and is much less intensive in the visible light region. Fluorescence is always weaker than the radiation scattered in an emulsion. The measurement and modelling of ultraviolet radiation scattering requires fluorescence to be taken into consideration, but in the visible spectral region this phenomenon is negligible.
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