Ograniczanie wyników

Czasopisma help
Autorzy help
Lata help
Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 23

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  legume seed
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
Na-phosphatc buťťcr extracts from seed coat of faba bean, horse bean, lentil and pea showed superoxide radical anion scavenging effect in the xanthine-xanthine oxidase system. The potential hierarchy for scavenging superoxide radicals in investigated seed coats extracts appears to be as follows: lentil>faba bean>pea>horse bean. Superoxide scavenging activity of extracts showed statistically insignificant correlation with the total antioxidative activity (r = 0.64; p≤0.05). Extracted and partly purified (with the use of Sephadex G-75) seed coat proteins of pea revealed the activity of superoxide dismutase (SOD). Electrophoretic separation of protein fractions showing antioxidative activity conducted in denaturating conditions (SDS-PAGE) showed the presence of proteins of molecular weights lower than 66 kDa, whereas the predominant proteins in both active fractions were those of high molecular weights (approx. 600 kDa) as it was found by gel filtration chromatography in FPLC system. In these two active fractions phenolic compounds were detected, so it can be supposed that they can interact with protein and occur as protein-phenolic compound complexes of high molecular weight.
Beside RFOs, which are commonly present in legume seeds, seeds of some species contain galactosyl pinitols (GPs). These carbohydrates, like RFOs, have been hypothesized to constitute an important energy and carbon skeletal source during germination. To test this hypothesis we have applied a specific α-galactosidase inhibitor (1-deoxygalactonojirimycin, DGJ) to germinating winter vetch (Vicia villosa Roth.) seeds, containing more galactosyl pinitols than RFOs. The breakdown of RFOs but not that of GPs was completely blocked in both embryonic axes and cotyledons tissues, during the first 18 h of imbibition in DGJ. The inhibitor only decreased the rate of GPs degradation. The inhibitory effect of DGJ on GPs degradation was partially alleviated by addition of sucrose or galactose to DGJ solutions. After three days of germination in water, RFOs and GPs disappeared in axial tissues of seeds imbibed in water, galactose or sucrose. Eighteen-hour imbibition of seeds in DGJ drastically reduced germination, by ca 50%, during the first three days. The inhibitory effect of DGJ decreased during the next seven days of germination. The presence of galactose or sucrose in imbibition solution initially stimulated seed germination, but later this effect was not statistically significant. Our study provides clear evidence that galactosyl pinitols play an important role in early winter vetch seeds germination. Additionally, we suggest that galactosyl pinitols can replace RFOs as reserve material necessary for early germination.
The leguminous seeds constitute a valuable source of feed and dietary protein, however, they contain also many different substances that are recognised as antinutritive factors. This review deals with harmful substances and composition of leguminous seeds, their profitable and negative activity considering also the effect on physiology of digestion, intestinal functions and health of animals.
The aim of the presented research was to determine the activity and interactions of the human intestinal microflora with biologically-active compounds and to ascertain the degree of its “in vitro” survivability in the applied model of the gastrointestinal tract. Products (noodles) from colour bean seeds cv. Red Kidney were obtained after thermoplastic treatment with addition of plant extracts: garlic, onion and oregano (concentration of aqueous extracts 50 mg/mL). The highest antioxidant capacity (22.88 mg Tx/g) as well as content of phenolic compounds (2.94 mg/g) after the digestion process was determined for noodles from red bean with the onion extracts. The growth of investigated bacteria (Enterobacteriaceae, Lactobacillus, Enterococcus, Bifidobacterium genera) was affected by the digested noodles from red bean with the plant extracts, especially of the Enterococcus and Bifidobacterium species. The amount of bacteria after digestion in the large intestine maintained at 108 cfu/mL, whereas the noodles without the addition of plant origin extracts also stimulated the growth of microflora. In effect, the amount of bacteria detected in the large intestine accounted for 109 – 1010 cfu/mL.
The objective of this work was to use the fluorometric method to study the antioxidative properties of the preparations obtained from the seed coats of red and brown beans. The preparations were obtained by extraction of the polyphenols with 0.5% HCl solution in methanol, vacuum concentration and lyophilisation. The qualitative and quantitative compositions of the preparations were determined. The antioxidative properties of polyphenolic preparations were determined by measuring the fluorescence as produced by the formation of hydroxide radicals from hydrogen peroxide and sodium benzoate in the presence of copper ions and dithioerythritol (DTET) in phosphate buffer, pH 7.2. The fluorescence of hydroxide derivatives was measured spectrofluorometrically. The studies showed that the preparations had the capabilities to scavenge the hydroxide radicals by 51% in the case of brown pigments and by 47% in the case of red pigments.
The objective of the study was to compare parallel immunoreactive properties and sensory characteristics of lentil (Lens culinaris) and mung bean (Vigma radiata L.) sprouts. The seeds were germinated with and without light at 20°C up to 3 days. The changes in immunoreactivity of the sprouts were determined by competitive ELISA method using rabbit polyclonal antisera to lentil and mung bean proteins. The sensory quality of samples was evaluated by quantitative descriptive analysis (QDA). In QDA a trained panel (n=9) rated the sprouts for odour, taste and texture. The results indicated that the effects of germination on the immunoreactive properties of sprouts with cotyledons depended on the type of legumes. The presence or absence of light during the germination process did not affect the results achieved. The removal of the cotyledons almost completely reduced immunoreactivity of samples (97% for lentil and 99% for mung bean). The effects of germination on the sensory profile of sprouts were found to depend on the type of legumes but as far as the sprouts of mung bean are concerned also on the processing conditions. The QDA demonstrated significant differences between the sprouts for the following attributes: bitterness (p<0.001), “pea-pod” taste (p<0.01), flourness (p<0.001) and juiciness (p<0.01).
The influence of pepsin (EC 3.4.1.1) and trypsin (EC 3.4.4.4) action on the chemical composition of legume flours was the aim of this study. The level of proteins and lipids in hydrolysed flours was changed significantly. In comparison to the raw flours also fatty acid composition in treated flours was altered. In the lentil flours both trypsin and pepsin digestion conditions have decreased the level of unsaturated fatty acid. It is noteworthy that in all investigated, hydrolysed flours ratio linoleic: oleic fatty acid was significantly decreased in comparison to unhydrolysed flours (about 40%-pea; 60%-lentil). Our investigations were also focused on the potential implementations of IMAC method in the separation and purification of peptides. Generally, peptides separation profiles, performed on immobilized Zn (II), were dependent on the kind of flour and enzyme used in the hydrolysis process. In the lights of our results is clearly visible that investigated peptides had a weak affinity to the chelated metal ions. It is noteworthy, that in some cases the influences of chelating factor on separation profiles were noticeable.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.