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Rhizobium strains isolated from nodules of the different legumes including wild-growing plants were examined for their siderophore activity. Fifteen of the 84 screened rhizobial strains were able to grow under conditions of limited iron supply. Nine of them gave orange halos in the assay with Chrom azurol S. Among these strains were Rhizobium sp. (Ononis) and Rhizobium (Genista), producing hydroxamates and phenolates. These compounds could promote the growth of siderophore-negative bacteria on iron-deficient media. The results imply that the hydroxamates from G1 and Ol strains may belong to the monohydroxamate class of siderophores.
The paper showed the applicability of the PCR method to an early identification of Fusarium avenaceum and Fusarium culmorum in infected tissues of selected crops. The polymerisation chain reaction used species-specific SCAR primers. There was observed a discrepancy between the size of multiplied DNA of Fusarium avenaceum fragment and that of Fa-U17f 5’, Fa-U17r earlier described by authors of primers. The present research product size for F. avenaceum was 950 bp, while for F. culmorum - 472 bp which confirmed the reports by other authors.
Although diversity-ecosystem theory predicts that ecosystem functioning is strongly determined by species number, species traits play an important role in regulating ecosystem-level dynamics. We analyze responses of species attributes to diversity level and resource availability, and explore their consequences for ecosystem functioning and ultimately assess the contributions of five traits (vegetative plant height, clonal growth, root depth, cespitose habit and seed mass) to ecosystem functioning defined by spatial stability of community biomass. We found that functional traits disproportionately affected spatial stability. Relationships between species functional traits and spatial stability of community biomass indicated that diversity of vegetative plant height facilitated stability of a nitrogen fertilized undisturbed natural community (NAT), and that of a phosphorus fertilized forb, legume and bunchgrass community (FLB). The clonal growth form was also identified as a stabilizing trigger for a unfertilized undisturbed natural community (NAT), whereas diversity in root depth, cespitose habit and seed mass were related to destabilization of a nitrogen fertilized rhizomatous grass community (RRR). Studies quantifying interactions among plant traits, community structure and ecological functioning will contribute much more to understanding of the effects of the ecological behavior of specific traits on the ecosystem functioning.
 Two early nodulin 40 (enod40) genes, ENOD40-1, the shortest legume ENOD40 gene, and ENOD40-2, were isolated from Lupinus luteus, a legume with indeterminate nodules. Both genes were expressed at similar levels during symbiosis with nitrogen-fixing bacteria. ENOD40 phylogeny clustered the L. luteus genes with legumes forming determinate nodules and revealed peptide similarities. The ENOD40-1 small ORF A fused to a reporter gene was efficiently expressed in plant cells, indicating that the start codon is recognized for translation. The ENOD40-1 RNA structure predicted based on Pb(II)-induced cleavage and modeling revealed four structurally conserved domains, an absence of domain 4 characteristic for legumes of indeterminate nodules, and interactions between the conserved region I and a region located upstream of domain 6. Domain 2 contains Mg(II) ion binding sites essential for organizing RNA secondary structure. The differences between L. luteus and Glycine max ENOD40 RNA models suggest the possibility of a switch between two structural states of ENOD40 transcript.
Changes in the amino acid profile of protein during ensiling depend on the proteolytic potential of plant species, the rate of the wilting and acidifying of ensiled herbage and, on the other hand, on the microbiological processes and fermentation of amino acids. The interaction of these factors determines the subsequent efficiency of nitrogen utilization in the rumen. The aim of this study has been to determine the influence of ensiling in round bales on changes in the amino acid content in lucerne and red clover silages. The amino acid composition was determined by analyzing the content of amino acids in herbages and silages. The lucerne silage protein was characterized by a higher contribution of all amino acids except Trp in comparison with red clover silage. Ensiling in bales of wilted lucerne and clover herbage lowered the content of Asp, His (P < 0.01), Ile, Lys (P < 0.05) in both plants. The ensiling process and plant species affected (P < 0.05) the total content of amino acids per 100 g of protein, where the range of changes in both raw materials was different. The loss of amino acids observed during the ensiling of wilted lucerne in round bales should be regarded as typical, whereas changes in the amino acid profile of red clover should be considered as atypical. A significantly higher ADIN content in red clover than in lucerne silage indicates that a rapid and significant increase of temperature occurred in experimental bales, which were characterized by a low degree of density, and this temperature rise strongly affected the availability of amino acids of the red clover protein also to the current microflora in the silage. Ensiling in round bales changed the amino acid profile of the lucerne and red clover protein. The ensiling process in this technology, however, severely deteriorated the quality of the red clover protein by lowering the content of all essential amino acids except Cys and Met.
Legumes and herbs are valuable elements of the meadow sward. In the conditions of extensive meadow-pasture management, for example in protected areas where first cut harvest is delayed, they determine to a large extent fodder quality, for instance total protein content. The aim of the study was the evaluation of total protein and crude fibre contents, as well as dry matter digestibility of chosen legume and herb species from extensive grasslands. Research was conducted in the years 1998-2000 before first cut harvest (June – first ten days of July) in the Olsztyn Lake District. Together, 444 plant samples were studied. The determinant for sample uptake location was the occurrence of phytocenoses with at least 5% area coverage (2 in the Braun-Blanquet scale) with chosen legume species: Trifolium pratense L., Trifolium repens L., Lotus corniculatus L., Lathyrus pratensis L., Lotus uliginosus Schkuhr., and Vicia cracca L., as well as herbs: Taraxacum officinale F. H. Wigg., Achillea millefolium L., Plantago lanceolata L., Alchemilla vulgaris L., Heracleum sibiricum L., and Cirsium oleraceum (L.) Scop. Average total protein content in the legumes oscillated between 176.9 and 196.6 g·kg⁻¹. Total protein content in herbs usually reached 100-120 g·kg⁻¹ for Taraxacum officinale F.H. Wigg., Achillea millefolium L. and Alchemilla vulgaris L. and 140-160 g·kg⁻¹ for Heracleum sibiricum L. and Cirsium oleraceum (L.) Scop. Research demonstrated that the legumes contained significantly more total protein and crude fibre than herbs, where no significant differences were found between the particular legume species. However, in the case of herbs, significant diversification of those components occurred, and the highest amount of protein and at the same time the lowest amount of fibre was accumulated by Heracleum sibiricum L. and Cirsium oleraceum (L.) Scop. Legume digestibility reached 60-65%. Among the herbs, higher digestibility was characteristic for Heracleum sibiricum L. (74.1%) and Cirsium oleraceum (L.) Scop. (74.4%), whereas the lowest one for Achillea millefolium L. (62.2%).
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