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A rapid system for studying foreign gene expression in wheat [Triticum aestivum L.]

100%

Ortiz J. P. A., Ravizzini R. A., Morata M. M., Vallejos R. H.

Journal of Applied Genetics

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1997

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tom 38

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nr 2

The present work describes a rapid and easy way to study foreign gene expression in wheat through microparticle bombardment. Transient expression of the GUS reporter gene was evaluated in different tissues of the cultivar Buck Ombú. Transformation was carried out employing a helium gun microparticle accelerator and several plasmids. Embryogcnic calli, immature embryos and immature inflorescences showed a higher number of transformed cells per Petri dish. Basal leaf segments were the least efficient. When vectors were tested in scutellar tissue of immature embryos, the maize ubiquitin promoter (Ubil) produced the highest level of transient GUS expression, followed by the alcohol dehydrogenase promoter of maize plus its first intron (Adh1 Adh-intron 1) and the sunflower ubiquitin promoter. The CaMV35S promoter was the least effective. These results were in agreement with previous reports carried out in cell suspensions or embryogenic calli and indicate that wheat immature embryos and immature inflorescences are suitable for rapid test of promoter sequences or transformation conditions. Moreover, efficient transformation of these explants could help in the stable transformation of agronomically important genotypes.

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Capillary zone electrophoresis for determination of reduced and oxidised ascorbate and glutathione in roots and leaf segments of Zea mays plants exposed to Cd and Cu

67%

Dresler S., Maksymiec W.

Acta Scientiarum Polonorum. Hortorum Cultus

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2013

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tom 12

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nr 6

The concentration of non-enzymatic antioxidants such as ascorbate and glutathione in tissues is one of the major plant responses to biotic and many abiotic stresses, including metals. Therefore, it is crucial to develop the most effective methods for simultaneous quantitative analysis of these antioxidants. Capillary zone electrophoresis allows relatively fast and effective analysis. The aim of the paper was to apply and optimise the capillary electrophoresis conditions for simultaneous determination of glutathione, glutathione disulphide, ascorbate, and dehydroascorbate in small plant tissue samples exposed to copper and cadmium. The method ensures good linearity and reproducibility, with correlation coefficients 0.988 for ascorbate and 0.999 for glutathione and glutathione disulphide, and with detection limits approximately 2.50, 0.65 and 0.50 ppm, respectively. Cu stress was found to increase the ascorbate concentration and glutathione content in leaves, while Cd increased glutathione in the oldest leaf segments and root.

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Formation of adventitious buds on the leaves of Aesculus hippocastanum L. cultured in vitro

59%

Wojciechowicz M. K., Mol R.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1992

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tom 40

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nr 1

Ograniczanie wyników

1 Acta Scientiarum Polonorum. Hortorum Cultus

1 Bulletin of the Polish Academy of Sciences. Biological Sciences

1 Journal of Applied Genetics

1 Dresler S.

1 Maksymiec W.

1 Mol R.

1 Morata M. M.

1 Ortiz J. P. A.

1 Ravizzini R. A.

1 Vallejos R. H.

1 Wojciechowicz M. K.

1 2013

1 1997

1 1992

A rapid system for studying foreign gene expression in wheat [Triticum aestivum L.]

Capillary zone electrophoresis for determination of reduced and oxidised ascorbate and glutathione in roots and leaf segments of Zea mays plants exposed to Cd and Cu

Formation of adventitious buds on the leaves of Aesculus hippocastanum L. cultured in vitro