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The bio-efficacy of four plant leaf extracts viz., apple of sodom, Calotropis procera Aiton; Mexican poppy, Argemone mexicana Linnaeus; Mexican marigold, Tagetes minuta Linnaeus and Indian neem, Azadirachta indica were tested against mustard aphid, Lipaphis erysimi on Indian mustard, Brassica juncea. The highest per cent aphid reduction during first, second and third spray were 28.79, 40.52 and 59.32 at 1 : 10 g/ml; 34.70, 44.49 and 66.14 at 1 : 5 g/ml and 53.88, 64.84 and 100.00 at 1 : 2.5 g/ml with Indian neem. However, Mexican marigold was also effective at highest concentration (1 : 2.5 g/ml) and reduced 96.38 per cent L. erysimi. All the treatments of plant leaf extracts showed insecticidal activity, but Indian neem followed by Mexican marigold reduced the aphid population to a great extent.
Jasminum auriculatum belonging to the family Oleaceae are reported to have good medicinal values in traditional system of medicines. The present study deals with pharmacognostical examination of morphological and microscopical characters and phytochemical investigations of Jasminum auriculatum leaves including determination of loss on drying, ash values and extractive values. The preliminary phytochemical screening of powdered drug was also carried out, the qualitative chemical examinations revealed the presence of various phytoconstituents like alkaloids, steroids, terpenoids, saponins, carbohydrates, Glycosides, protein, mucilages, phenols ,tannins and flavonoids.
The aim of this study was to evaluate the effects of dietary supplementation of bamboo leaf extract (BLE) on the growth performance, antioxidant traits, immune function, and lipid metabolism of weaning piglets. After weaning at 21 days, two hundred and forty healthy piglets (Large White × Landrance) were randomly assigned to 4 treatments with 6 pens and 10 piglets per pen. The control group (Ctr) received a maize-soyabean based diet, and the test groups received the control diet plus 0.5%, 1%, or 2% (w/w) BLE, respectively. The experiment was carried out for 5 weeks. At the end of it, average daily feed intake in the 1% BLE group was decreased (p < 0.05). Plasma concentrations of malondialdehyde were decreased with supplementation of 1% and 2% BLE. Immunoglobulin G concentrations and lysozyme activity in plasma were significantly increased in piglets supplemented with BLE. Diets with 1% and 2% BLE increased (p < 0.05) plasma concentrations of low density lipoprotein-cholesterol and reduced (P < 0.01) high density lipoprotein-cholesterol levels. Higher triglyceride concentrations were observed in the 0.5% (P < 0.01) and 2% (p < 0.05) BLE groups. In conclusion, these novel findings demonstrate that supplementation of BLE to the diet improved the antioxidant activity, immune function, and lipid metabolism of weaning piglets.
In the present study Limonia acidissima Groff. ethanolic leaves extract was used for the detection of its antidermatophytic assay. It results broad spectrum of antifungals and antibacterial. Where the antimycotic activity against Trichophyton rubrum, Trichophyton tonsurans, Trichophyton mentagrophytes, Microsporium gypseum, Candida albicans and four Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa were more pronounced. The effective antidermatophytic activity observed against M. gypseum, T. tonsurans, E. coli and B. subtilis. Minimum Inhibitory Concentration of all the test microbes was determined using broth dilution technique. MFC, MBC also standardized. Preliminary Phytochemical tests were carried for the revealing basics antimicrobial responsible molecules of secondary metabolites.
Terminalia chebula, a plant rich with phytochemicals, was selected in the present investigation for evaluating its immunomodulatory activities. Extract of the leaves were prepared in various solvents. Ethanolic extract was found to contain significant amount of phytochemicals. Hence, it was selected to study immunomodualtory activity. Silica gel column chromatography of ethanolic extract was performed. The fractions were further subjected to TLC and most active fractions were administrated to balb/C mice for evaluating immunomodulatory studies. Out of the five fraction (S1 – S5), S3 was found to possess immunostimulant activity. Provision of Ethanol Extracts of Terminalia chebula on balb/C mice can increase the antibody titers IgM and IgG.
Methanol and ethanol extract showed antibacterial activity in range of 0-11 at 10 mg/mL at against all tested bacterial species. Methanol extract with amoxicillin exhibited effective inhibition E. coli. This combination showed antagonistic effect against B. subtilis and S. aureus. Interaction of ethanol extract with amoxicillin also showed effective inhibition against E. coli and decreased activity against B. subtilis and S. aureus. Methanol extract did not show inhibition against P. aeruginosa but it enhanced activity of ciprofloxacin on combination. Ethanol extract also exhibited effective inhibition against P. aeruginosa on combination with ciprofloxacin. But antagonist effect was observed on interaction of both extract with ciprofloxacin against other bacterial species. Interaction of methanol and ceftazidime showed mostly decreased or indifferent effect against all bacterial species. Ethanol extract with ceftazidime resulted in higher inhibition against E. coli. Combination of methanol extract with erythromycin showed higher inhibition against E. coli and also very effective inhibition against S. aureus. This combination resulted in decreased activity against B. subtilis. Ethanol extract with erythromycin also showed synergistic effect against S. aureus and effective inhibition against E. coli. These results indicate that both methanol and ethanol extract has potential to enhance the combination effect with erythromycin against S. aureus and E. coli.
The present investigation was carried out to evaluate the hypolipidemic activity of methanolic and aqueous leaf extracts of Cassia fistula (CF) Linn (Family: Fabaceae) on atherogenic diet (2% cholesterol, 1% Choline Chloride and 2% Lard) induced Male Sprauge-Dawley rats for the period of 21 days. The rats receiving the treatment with leaf methanol and aqueous extract of CF showed significant (P < 0.001) reduction in total cholesterol (TC), triglycerides (TG), High density lipoprotein (HDL), lipid peroxidation (LPO) and other parameters at dose dependent manner but the effect was less than the standard drug Atorvastatin. The results revealed the effectiveness of CF plant against hyperlipidemic activity.
Antimycotic activity of Petroleum ether and 98% methanolic young leaf soxhlet extract of Solanum nigrum (Solanaceae) was evaluated against dermatophytic fungi namely, Trichophyton rubrum, Trichophyton tonsurans, Trichophyton mentagrophytes, Microsporium gypseum, Candida albicans, and bacteria like, Staphylococcus aureus, Psudomonas aeruginosa, Bacillus subtilis, Escherichia coli. The maximum activity was observed in interpolar methanolic extract when compared to low polar petroleum ether extract. The minimum inhibitory concentration, minimum fungicidal concentration and minimum bactericidal concentration were determined against all the test strains. This study provides a basis for the isolation and purification of anti-dermatophytic compounds from the young leaves of S. nigrum.
In pancreatic β-cells, although H2O2 is a metabolic signal for glucose stimulated insulin secretion, it may induce injury in the presence of increased oxidative stress (OS) as in the case of diabetic chronic hyperglycemia. Olea europea L. (olive) leaves contain polyphenolic compounds that may protect insulin-secreting cells against OS. The major polyphenolic compound in ethanolic olive leaf extract (OLE) is oleuropein (about 20 %), thus we compared the effects of OLE with the effects of standard oleuropein on INS-1 cells. The cells were incubated with increasing concentrations of OLE or oleuropein for 24 h followed by exposure to H2O2 (0.035 mM) for 45 min. H2O2 alone resulted in a significantly decreased viability (MTT assay), depressed glucose-stimulated insulin secretion, increased apoptotic and necrotic cell death (AO/EB staining), inhibited glutathione peroxidase activity (GPx) and stimulated catalase activity that were associated with increased intracellular generation of reactive oxygen species (ROS) (fluorescence DCF). OLE and oleuropein partly improved the viability, attenuated necrotic and apoptotic death, inhibited the ROS generation and improved insulin secretion in H2O2-exposed cells. The effects of oleuropein on insulin secretion were more pronounced than those of OLE, while OLE exerted a stronger anti-cytotoxic effect than oleuropein. Unlike OLE, oleuropein had no significant preserving effect on GPx; however, both compounds stimulated the activity of catalase in H2O2-exposed cells. These findings indicate different modulatory roles of polyphenolic constituents of olive leaves on redox homeostasis that may have a role in the maintenance of β-cell physiology against OS.
Reaumuria soongorica (Pall.) Maxim., a perennial semishrub, is able to survive almost complete tissue dehydration when water is withheld from it, and then the stem can rehydrate on rewatering. In this work, a series of experiments were conducted to characterize the drought-induced changes in two-year-old Reaumuria soongorica. The plants were subjected to dehydration by withholding water for 15 days. Net photosynthetic rate (Pn), maximal photochemical efficiency of photosystem (Fv/Fm) and the activity of ribulose-l,5-bisphosphate carboxylase (RuBPCO) were significantly decreased under drought stress, but phosphoenolpyruvate carboxylase (PEPCase) activity increased in the leaf extracts. Content of chlorophylls and carotenoids had no marked variation. Zeaxanthin, the xanthophyll cycle pigment, increased during drying. Plants exposed to drought showed accumulation of sucrose, with lower soluble protein content than in the control plants. The SDS-PAGE protein profile showed a 52 kD polypeptide disappearing under progressive drought stress, but no drought-induced protein occurred. All these findings indicate that the metabolic network systems of Reaumuria soongorica have a robust regulation capability for management of severe drought stress.
Many experimental studies have demonstrated the favorable biological activities of plants belonging to the genus Rubus, but little is known of the role of Rubus leaf extracts in the modulation of the surface membrane expression and activity of endothelial apyrase. The aim of this study was to assess the influence of 1–15 μg/ml Rubus extracts on CD39 expression and enzymatic activity, and on the activation (ICAM-1 expression) and viability of human umbilical vein endothelial cells (HUVEC). The polyphenolic contents and antioxidative capacities of extracts from dewberry (R. caesius L.) and raspberry (R. idaeus L.) leaves were also investigated. The techniques applied were flow cytometry (endothelial surface membrane expression of ICAM-1 and CD39), malachite green assay (CD39 activity), HPLC-DAD (quantitative analysis of polyphenolic extract), ABTS, DPPH and FRAP spectrometric assays (antioxidant capacity), and the MTT test (cell viability). Significantly increased CD39 expressions and significantly decreased ATPDase activities were found in the cells treated with 15 μg/ml of either extract compared to the results for the controls. Neither of the extracts affected cell proliferation, but both significantly augmented endothelial cell ICAM-1 expression. The overall antioxidant capacities of the examined extracts remained relatively high and corresponded well to the determined total polyphenol contents. Overall, the results indicate that under in vitro conditions dewberry and raspberry leaf extracts have unfavorable impact on endothelial cells.
Bio-stimulants are in excessive demand for the sustainable production of floriculture crops. The current investigation was designed to find out the effect of naturally occurring growth stimulant moringa leaf extract (MLE) on the growth, flowering, post-harvest life and corm production of two gladiolus cultivars (cvs.) ‘Rose Supreme’ and ‘White Prosperity’. The research trial was laid out in the factorial arrangement under randomized complete block design. Corms were grown in the open field under local climatic conditions of Faisalabad, Pakistan. Potential of exogenously applied (MLE; 30 times diluted) was evaluated. Treatments including spraying MLE at 3 leaves, 5 leaves, floral bud stage, one combination of these three stages relative to control with no foliar application. Application of natural bio-stimulant produced maximum height in both cvs. against T4. Both cvs. gave maximum stalk length in response to T4. The number of florets spike–1 found the maximum (13) in T1 compared to control T0 which yield (9) florets. Maximum vase life in sucrose solution (13.33 days) was exhibited by T1 for both cvs. while minimum (10 days) in T0. In cultivar comparison earlier spike emergence was observed in ‘White Prosperity’. In ‘Rose Supreme’ maximum corm weight was attained in response to T3 (43.43 g) while minimum (30.33 g) in T0.‘White Prosperity’ produced maximum weight (40.33 g) against T4 whereas minimum by control. The cultivar mean comparison showed the superiority of cormel diameter in ‘Rose Supreme’ (10.93 mm) than ‘White Prosperity’ (9.13 mm). In treatment comparison, T4 produced maximum diameter (12.04 mm) in ‘Rose Supreme’ moreover, T2 induced maximum (9.57 mm) diameter in ‘White Prosperity’.
Th e present exploration is focused on the bio-fabrication of silver nanoparticles (Ag NPs) using Trichodesma indicum aqueous leaf extract as a reducing agent. Th e synthesized Ag NPs were productively characterized by UV-vis spectroscopy, XRD, and TEM studies. Th e photosynthesis of Ag NPs was done at room temperature for 24 h and at 60°C. Th e green synthesis of spherical-shaped Ag NPs bio-fabricated from T. indicum with a face centred cubic structure showed average particle sizes of 20–50 nm, which is inconsistent with the particle size calculated by the XRD Scherer equation and TEM analysis. We further explored the larvicidal effi cacy of biosynthesized Ag NPs with leaf extracts of T. indicum against Mythimna separata. Th e results showed that Ag NPs (20–50 nm) of T. indicum possess good larvicidal activity against M. separata with an LC50 of 500 ppm. Th us, we can advocate that Ag NPs of 20–50 nm size extracted from T. indicum may be considered in the pest management programme of M. separata in future.
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