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Indirect shoot regeneration from leaf explants of Kalanchoe pinnata

100%

Andrzejewska K., Durska A., Kempa M., Kopec P., Sobusiak A., Stec N., Trzebny A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2015

|

tom 96

|

nr 1

2

Protuberance-mediated high frequency of adventitious shoot regeneration from sweetpotato leaf explants [Ipomoea batatas (L.) Lam.]

100%

Ashok Kumar H. G., Ku A. T., Agrawal P., Mahesh S., Yeh K.-W.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2013

|

tom 94

|

nr 4

3

Induction, maintenance and preservation of embryogenic competence of Gentiana cruciata L. cultures

100%

Mikula A., Fiuk A., Rybczynski J. J.

Acta Biologica Cracoviensia. Series Botanica

|

2005

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tom 47

|

nr 1

The paper describes structural and ultrastructural changes in primary explants, induction of embryogenesis, somatic embryo development, and four protocols for cryopreservation of cell suspensions. The changes during tissue culture of hypocotyl and cotyledon explants from 10-day-old seedlings and fragments of leaf explant of Gentiana cruciata L. were studied. Seedling explants were cultured on MS medium supplemented with 1.0 mg/l dicamba + 0.1 mg/l NAA + 2.00 mg/l BAP + 80.0 mg/l adenine sulphate. The hypocotyl callus tissue was initiated by cell divisions of the vascular cylinder, but in cotyledons only parenchyma cells took part in callus formation. The leaf blade expiants usually responded only by proliferation of the wounded surface. The effect of auxins (2,4-D, NAA, DIC) and cytokinins (kinetin, zeatin, BAP) in various concentration and combinations on leaf explant response was examined. Generally, embryos were formed sporadically on media containing NAA (1.64% responding explants) or 2,4-D (0.38%), but were not produced in the presence of dicamba. Production of somatic embryos was more effective from suspension culture than from agar medium. Liquid culture made it possible to maintain the cell suspension’s embryogenic competence for 5 years. For preservation of proembryogenic masses, four protocols of cryopreservation were studied: direct cooling, sorbitol/DMSO treatment, vitrification, and encapsulation. Direct cooling and sorbitol/DMSO treatment was unsuccessful. Vitrified tissue required a minimum 3 weeks of culture on solid medium for cell proliferation to reach the proper fresh weight for manipulation. Alginate beads with PEMs were transferred directly to liquid medium for post-freezing culture. Vitrification and encapsulation maintained high viability of post-freezing PEM, but encapsulation ensured faster restoration of G. cruciata cell suspension.

4

In vitro regeneration induced in leaf explants of Citrus limon L. Burm cv. ‘Primofiore’

84%

Kasprzyk-Pawelec A., Pietrusiewicz J., Szczuka E.

Acta Scientiarum Polonorum. Hortorum Cultus

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2015

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tom 14

|

nr 4

In vitro organogenesis was studied using Citrus limon L. Burm cv. ‘Primofiore’ leaf explants. The purpose of the present study was to optimize conditions for callogenesis and organogenesis of C. limon. Explants of C. limon were cultured on 16 different media supplemented with various combinations of plant growth regulators, both auxins and cytokinins, such as 6-benzylaminopurine (BAP), naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and kinetin. The best shoot induction was obtained when the leaf explants were cultured on Murashige & Tucker media supplemented with 3.5 mg·L-1 BAP. Histological investigation revealed most likely the initial phase of development of leaf explants during in vitro regeneration of C. limon. t

5

The course and efficiency of organogenesis on leaf explants of plum and acute; Wegierka Zwykla and acute; [Prunus domestica L.] induced by cytokinins

84%

Nowak B., Miczynski K.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

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2002

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tom 05

|

nr 1

6

In vitro regeneration and somaclonal variation of Petunia hybrida

84%

Abu-Qaoud H., Abu-Rayya A., Yaish S.

Journal of Fruit and Ornamental Plant Research

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2010

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tom 18

|

nr 1

71-81

The effect of different levels of naphthaleneacetic acid (NAA) and benzyladenine (BA) on shoot multiplication and regeneration of Petunia hybrida was studied. Regenerated shoots from leaf explants were grown ex vitro for analysis of somaclonal variation. Seeds of Petunia hybrida were germinated in vitro on MS basal medium. The seedlings were used as a source of explants for the multiplication and regeneration experiments. For the shoot multiplication experiment, shoot explants taken from germinated seedlings were cultured on MS basal media supplemented with different concentrations of BA (0.1, 0.4, 0.8 mgl-1) and NAA at concentration of 0.1 mg l-1. Highest number of axillary shoot was obtained on medium supplemented with 0.8 mg l-1 BA and 0.1 mgl-1NAA. For regeneration experiments, leaf sections taken from germinated seedlings were cultured onto MS media supplemented with three levels of BA (0.5, 1.0 and 2.0 mgl-1) and 0.5 mgl-1NAA. The highest shoot regeneration rate (45%) was observed in MS medium supplemented with 2 mg l-1 BA. For the evaluation of somaclonal variation, lateral bud explants were taken from pink colored petunia plants that were grown in the greenhouse. The buds were disinfected and cultured on MS basal media supplied with 30 mgl-1 gentamycin sulfate and 30 mg l-1 Benlate. After shoots grew, leaf sections were then taken from them shoots and cultured onto shoot regeneration medium (MS medium supplemented with 2 mg l-1 BA). The regenerated adventitious shoots were cultured on MS medium without growth regulator. These shoots were then rooted, acclimatized and transferred to the greenhouse for evaluation. Two forms of leaf shape (orbicular and elliptic) and three flower colors (violet, purple and light pink) appeared on the plantlets.

7

In vitro adventitious organogenesis in Ajania pacifica (Nakai) Bremer et Humphries

84%

Tymoszuk A., Antkowiak M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2018

|

tom 99

|

nr 4

8

Micropropagation of wild chicory (Cichorium intybus L. var. silvestre Bisch.) from leaf explants

84%

Dolinski R., Olek A.

Acta Scientiarum Polonorum. Hortorum Cultus

|

2013

|

tom 12

|

nr 6

Cichorium intybus is edible, medicinal and forage plant. The pharmaceutical raw materials were obtained from wild chicory (var. silvestre). Currently, farmers are increasingly assume plantations of wild chicory, and breeders are attempting to produce cultivars for medicinal purposes. In the modern breeding of chicory important feature is the ability to clonal propagation in vitro culture. The aim of our study was to assess capacity of natural population of wild chicory for plant regeneration from leaf explants. In the first was examined the effect of 16 combinations of various concentrations of IAA and 2iP on the regeneration of shoots from leaf explants (0.5 cm2 ). After that, 25 plants were propagated on the medium which was found as optimal. Then, their callus growth and shoots regeneration capacities were compared. Later on, was examined the effect of various IAA concentrations on the rooting of shoots. The majority of the shoots was regenerated from callus but direct organogenesis was also observed (8%). Shoot regeneration was found to be the most efficient on MS medium containing 0.5 mg dm-3 IAA and 4 mg dm-3 2iP – 97% of the explants produced shoots, while the average number of shoots was 15.5. The amount of callus was found to be a highly heritable trait (h2 = 0.83). Lower the heritability coefficients were obtained for the number of shoots per explant (0.55) and the average shoot weight (0.40). The wild chicory shoots rooted easily. The number and weight of roots increased with the increasing concentration of IAA.

9

Sodium nitroprusside stimulates micropropagation and TDZ induces adventitious shoots regeneration in red flesh apple Malus niedzwetzkyana Koehne Dieck ex

84%

Kazemi N., Jafarkhani Kermani M., Habashi A. A.

Journal of Horticultural Research

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2019

|

tom 27

|

nr 2

10

Somatic embryogenesis and plant regeneration from hypocotyl and leaf explants of Brassica oleracea var. botrytis (cauliflower)

84%

Siong P. K., Taha R. M., Rahiman F. A.

Acta Biologica Cracoviensia. Series Botanica

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2011

|

tom 53

|

nr 1

We investigated direct and indirect formation of somatic embryogenesis in Brassica oleracea var. botrytis (cauliflower), a very important vegetable crop worldwide. Direct somatic embryogenesis, which is rather rare, was achieved in culture of 2-week-old hypocotyl explants of Brassica oleracea var. botrytis on MS medium supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5; 1.0; and 1.5 mg/l kinetin. Initial induction of embryogenic callus was achieved on MS supplemented with very low concentrations of 2,4-D (0.05 mg/l and 0.1 mg/l). Indirect somatic embryogenesis from leaf sections was obtained on MS supplemented with 0.05 or 0.1 mg/l 2,4-D. We examined various stages of somatic embryos (globular, heart, torpedo, cotyledonary). More embryos per explant were produced through the indirect pathway (23–25) than through the direct pathway (14–19). The number of embryos produced was high. There is a potential for recurrent, repeated or secondary somatic embryogenesis, possibly an unlimited source for mass propagation and ideal for synthetic seed production in this species. Plant regeneration was achieved on half-strength MS medium without any hormones.

11

Somatic embryogenesis and plant regeneration from leaf explants of endemic Begonia pavonina

84%

Rosilah A. A., Kandasamy K. I., Faridah Q. Z., Namasivayam P.

Journal of Biology and Earth Sciences

|

2014

|

tom 04

|

nr 2

12

Induction of direct somatic embryogenesis and callogenesis in date palm (Phoenix dactylifera L.) using leaf explants

84%

Baharan E., Mohammadi P. P.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2018

|

tom 99

|

nr 3

13

Callus induction and Organogenesis in Sugarcane (Saccharum officinarum L.) var 93v297

84%

Rao A. S.

International Letters of Natural Sciences

|

2015

|

tom 48

An efficient protocol for induction of callus and regeneration of a sugar cane var 93v297 has been developed and reported here. Callus induction from immature young leaf explants derived from 2-3-month-old plants was achieved on Murashige and Skoog’s (MS) medium supplemented with different auxins viz, 2,4-D, NAA and IAA. Among different auxins, 2, 4-D at 3.5mg/l + 0.5mg/l BAP was found favourable in inducing callus. Addition of coconut milk and BAP further enhanced the growth of callus maximum being on MS medium supplemented with 0.5mg/l BAP (3602.33±0.88mg). Calli were further evaluated for regeneration. MS medium supplemented with 1.0 mg/l BAP was found suitable where 100% calli regenerated with maximum number of multiple shoots per callus mass (41.40±0.89). Highest number of root emergence (28.33±1.16) and maximum root length (3.40±0.67cm) was achieved on MS medium supplemented with 3mgl/l NAA. The in vitro grown plants were transferred to polycups containing a mixture of sterilized sand, soil and cocopeet (1:1:1) for hardening. The hardened plants were transferred to green-house conditions where they survived with 90% frequency.

14

Transgenic tobacco lines expressing levan sucrase

84%

Yordanov Y., Valkov V., Vassileva R., Atanassov A., Djilianov D.

Biological Bulletin of Poznań. Supplement

|

1997

|

tom 34

15

Selection for virus resistance in tomato exposed to tissue culture procedures

84%

Hanus-Fajerska E., Lech M., Pindel A., Miczynski K.

Acta Physiologiae Plantarum

|

2000

|

tom 22

|

nr 3

16

Evaluation of competent embryo production and conversion potential in callus cultures initiated in vitro from leaf explants of six sweet potato [Ipomoea batatas [L.] Lam] genotypes

84%

Gerszberg A., Macioszek V. K., Luchniak P., Kononowicz A. K.

Biotechnologia

|

2004

|

nr 1

17

Plant regeneration from leaf explants of Adromischus nussbaumerianus Poelln. and Echeveria laui Moran et Meyran

84%

Wojciechowicz M. K., Kazimierczak-Grygiel E., Olejnik S., Zenkteler E.

Biological Bulletin of Poznań

|

2001

|

tom 38

|

nr 1

18

The influence of the cooling of donor cultures on the in vitro adventitious regeneration and carbohydrate metabolism of four dwarfing apple rootstocks

67%

Orlikowska T., Zawadzka M., Kucharska D., Lahuta L. B.

Acta Physiologiae Plantarum

|

2010

|

tom 32

|

nr 2

We examined the effects of cooling applied for 4 to 20 weeks on donor cultures of four dwarfing apple rootstocks (P16, P22, P59 and M26). Our aim includes increasing their competence for in vitro adventitious shoot regeneration from the leaves. Donor cultures were maintained on a shoot multiplication medium at 4℃ in the dark for 4 months, followed by subculture on a fresh medium for 4 weeks. The cooling of the cultures caused an increase in the adventitious shoot number and a decrease in the starch content and an increase in the soluble sugar content (monosaccharides, raffinose and stachyose). The accumulation of stachyose in response to cold is a new observation, and it suggests that raffinose and stachyose play important role in the acclimation of dwarf apple rootstocks to low temperatures.

19

Embryogenic callus formation by cotyledon and leaf explants of Crambe abyssinica seedlings

67%

Furmanek T., Banas W.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2011

|

tom 92

|

nr 2

20

Adventitious shoot regeneration from leaf explants of Malus domestica Borkh. in vitro

67%

Podwyszynska M., Sowik I.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2015

|

tom 96

|

nr 1

Ograniczanie wyników

Indirect shoot regeneration from leaf explants of Kalanchoe pinnata

Protuberance-mediated high frequency of adventitious shoot regeneration from sweetpotato leaf explants [Ipomoea batatas (L.) Lam.]

Induction, maintenance and preservation of embryogenic competence of Gentiana cruciata L. cultures

In vitro regeneration induced in leaf explants of Citrus limon L. Burm cv. ‘Primofiore’

The course and efficiency of organogenesis on leaf explants of plum and acute; Wegierka Zwykla and acute; [Prunus domestica L.] induced by cytokinins

In vitro regeneration and somaclonal variation of Petunia hybrida

In vitro adventitious organogenesis in Ajania pacifica (Nakai) Bremer et Humphries

Micropropagation of wild chicory (Cichorium intybus L. var. silvestre Bisch.) from leaf explants

Sodium nitroprusside stimulates micropropagation and TDZ induces adventitious shoots regeneration in red flesh apple Malus niedzwetzkyana Koehne Dieck ex

Somatic embryogenesis and plant regeneration from hypocotyl and leaf explants of Brassica oleracea var. botrytis (cauliflower)

Somatic embryogenesis and plant regeneration from leaf explants of endemic Begonia pavonina

Induction of direct somatic embryogenesis and callogenesis in date palm (Phoenix dactylifera L.) using leaf explants

Callus induction and Organogenesis in Sugarcane (Saccharum officinarum L.) var 93v297

Transgenic tobacco lines expressing levan sucrase

Selection for virus resistance in tomato exposed to tissue culture procedures

Evaluation of competent embryo production and conversion potential in callus cultures initiated in vitro from leaf explants of six sweet potato [Ipomoea batatas [L.] Lam] genotypes

Plant regeneration from leaf explants of Adromischus nussbaumerianus Poelln. and Echeveria laui Moran et Meyran

The influence of the cooling of donor cultures on the in vitro adventitious regeneration and carbohydrate metabolism of four dwarfing apple rootstocks

Embryogenic callus formation by cotyledon and leaf explants of Crambe abyssinica seedlings

Adventitious shoot regeneration from leaf explants of Malus domestica Borkh. in vitro