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1

Aspergillus penicillioides Speg. implicated in keratomycosis

100%
Machowicz-Matejko E., Furmanczyk A., Zalewska E. D.
Polish Journal of Microbiology
|
2018
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tom 67
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nr 4
The aim of the study was mycological examination of ulcerated corneal tissues from an ophthalmic patient. Tissue fragments were analyzed on potato-glucose agar (PDA) and maltose (MA) (Difco) media using standard laboratory techniques. Cultures were identified using classical and molecular methods. Macro- and microscopic colony morphology was characteristic of fungi from the genus Aspergillus (restricted growth series), most probably Aspergillus penicillioides Speg. Molecular analysis of the following rDNA regions: ITS1, ITS2, 5.8S, 28S rDNA, LSU and β-tubulin were carried out for the isolates studied. A high level of similarity was found between sequences from certain rDNA regions, i.e. ITS1-5.8S-ITS2 and LSU, what confirmed the classification of the isolates to the species A. penicillioides. The classification of our isolates to A. penicillioides species was confirmed also by the phylogenetic analysis.
2

Clinical evaluation of tacrolimus eye drops for chronic superficial keratitis treatment in dogs

100%
Balicki I., Trbolova A.
Bulletin of the Veterinary Institute in Puławy
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2010
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tom 54
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nr 2
251-258
The aim of this study was to assess the efficacy of 0.02% tacrolimus ophthalmic drops application for chronic superficial keratitis (CSK) treatment in dogs. The studies included 14 German Shepherd dogs - eight males and six females, aged 2-10 years affected with CSK. The drops were administered to the ocular surface three times a day. Prior to the treatment onset, and after the 5 week medical therapy, an estimation of a conjunctiva redness, ocular discharge, depigmentation of the third eyelid, and blood vessel ingrowth in each corneal quadrant and corneal pigmentation was conducted. The photo images with calibrated grid enabled to calculate the percentage of corneal area surface affected by inflammatory process. Tacrolimus did not exert any irritant effects throughout the treatment. The therapy has led to the decrease in corneal inflammatory infiltrate and blood vessel ingrowth in all the patients. Median corneal area surface affected by the condition showed a statistically significant decrease from 46% to 27% (P<0.01) in case of the left corneas and from 58% to 33% for the right ones. Out of 27 corneas affected by pigmentation, 13 corneas in eight patients exhibited decreased pigmentation. The increased pigmentation was observed on eight corneas in five patients. The studies proved that 0.02% ophthalmic drops of tacrolimus have been effective topically in CSK therapy. A treatment response was observed by reduced granulation tissue and corneal neovascularisation, still in some cases tacrolimus failed to inhibit the pigmentation formation.
3

Pathological findings in cornea tissue of patients with penetrating keratoplasty

100%
Kostelna H., Rosocha J., Paulikova E., Kozak I., Antalova M., Mahel M., Harvanova D., Petrovicova J., Bobak L.
Folia Histochemica et Cytobiologica
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2010
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tom 48
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nr 2
267-272
4
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Feline herpesvirus-1: Ocular manifestations, diagnosis and treatment options

100%
Gould D.
Okulistyka Weterynaryjna. e-kwartalnik dla lekarzy i studentów weterynarii
|
2012
|
nr 2
W swojej pracy autor przedstawia kluczowe aspekty związane z chorobami oczu występującymi u kotów, które  powoduje  herpesvirus  koci typu 1. Praca  w obszerny sposób omawia wiele chorób implikowanych tym typem wirusa. szczegółowo opisuje objawy kliniczne poszczególnych schorzeń, diagnostykę oraz doradza w sposób praktyczny dobieranie odpowiedniego leczenia. Mamy podany szereg leków wraz z dawkami i sposobami ich podania dla poszczególnych schematów leczenia. Praca jest bogato ilustrowana fotografiami obrazującymi konkretne przypadki kliniczne. Autor włożył wiele wysiłku, dzięki czemu powstała naprawdę wspaniała praca naukowa, która praktykom daje gotowy schemat rozpoznawania i leczenia tych trudnych z punktu klinicznego chorób.
5

In vitro amoebicidal activity of imidazolium salts against trophozoites

100%
Fabres L. F., da Costa Goncalves F., Duarte E. O. S., Berte F. K., Silva da Conceicao D. K., Ferreira L. A., Schrekker H. S., Rott M. B.
Acta Parasitologica
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2020
|
tom 65
|
nr 2
6

Comparative analyses of different genetic markers for the detection of Acanthamoeba spp. isolates

100%
Derda M., Wojtkowiak-Giera A., Hadas E.
Acta Parasitologica
|
2014
|
tom 59
|
nr 3
Acanthamoeba are widespread free-living amoebae which may cause granulomatous amoebic encephalitis (GAE), keratitis, skin ulcerations and disseminated tissue infection. An important diagnostic and prognostic factor for the treatment of infection is a quick and correct diagnosis of amoebae strains. The aim of our study was to develop a rapid method for detection and identification of pathogenic Acanthamoeba spp. strains from diagnostic material collected from water. In this study we analysed five amplification-based genetic markers (Aca 16S, Ac6/210, GP, JDP, Nelson) used for identification of pathogenic Acanthamoeba spp. strains isolated in water sources in Poland, Iceland and Sweden. Our results demonstrated the presence of pathogenic Acanthamoeba strains in tap water. PCR assay appeared to be a more rapid and sensitive method to detect the presence of amoebae than the limited conventional techniques. Based on our observations, we can confirm that the use of four out of five genetic markers (Aca 16S, Ac 6/210, JDP, GP, Nelson) may be helpful in identification of Acanthamoeba spp. strains, but only one Aca 16S primer pair is a highly specific marker that distinguishes between pathogenic strains of Acanthamoeba and other free-living amoeba families.
7
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First cases of Acanthamoeba keratitis in Slovakia

100%
Ondriska F., Mrva M., Lichvar M., Ziak P., Murgasova Z., Nohynkova E.
Annals of Agricultural and Environmental Medicine
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2004
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tom 11
|
nr 2
We present the case report of the first identification of Acanthamoeba as a causative agent of keratitis in the Slovak Republic. For the first time, Acanthamoeba sp. Group III was isolated from a 53-year-old patient with keratitis, which was manifested after an injury of the right eye. A delayed visit to a physician as well as a late diagnosis of the illness led to the advanced stage of eye disease. As the treatment with itraconazol and cornea transplantation showed no result, enucleation of the eye was decided. Acanthamoeba ludgunensis was also the causative agent of keratitis in a 39-year-old patient wearing contact lenses. His complaints occurred a month after bathing in a thermal swimming pool. The symptoms presented in the left eye were those of herpetic keratitis, and led to a cloudy cornea with circular infliltrate and poor vision. A prompt clinical and laboratory diagnosis, along with treatment with propamidine-isetionate resulted in a significant improvement of the eye condition. Contact lenses were probably related to another case of Acanthamoeba keratitis. The patient, a 15-year-old girl, kept wearing contact lenses during bathing in various swimming pools and in the sea; her contact lenses were also regularly washed under tap water. Due to the fact that cysts of Acanthamoeba sp. group II were found in the contact lens solution, this is presumed to be the source of the eye infection.
8

The role of the innate and adaptive immune responses in Acanthamoeba keratitis

100%
Niederkorn J. Y.
Archivum Immunologiae et Therapiae Experimentalis
|
2002
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tom 50
|
nr 1
9

Susceptibility of Acanthamoeba to multipurpose lens-cleaning solutions

80%
Aguiar A. P. C., Oliveira Silveira C., Todero Winck M. A., Rott M. B.
Acta Parasitologica
|
2013
|
tom 58
|
nr 3
The present study investigated the susceptibility of Acanthamoeba spp. trophozoites to two multipurpose systems for cleaning and maintenance of contact lenses. Three strains of trophozoites from the ATCC (A. castellani T4, A. castellani Neff, and A. polyphaga) and two Acanthamoeba isolates obtained from swimming pools (PT5 and PO1) were placed in monoxenic culture. To test their survival in cleaning solutions for contact lenses, the trophozoites were exposed for 4 and 24 h to two multipurpose solutions (A and B), and were then inoculated into a new monoxenic culture. Amoebic growth on the plates was observed after 72 h of incubation. Trophozoites from all three ATCC strains and one isolate from a swimming pool (PO1) grew in all plates after 4 h of exposure to solutions A and B. After 24 h, the ATCC strains and the PO1 isolate showed growth in most of the plates treated. Only the PT5 isolate showed susceptibility to both solutions over the time intervals tested. The two solutions were not completely effective against most strains and isolates over the time intervals tested. These results are important, since species of Acanthamoeba are widely distributed in the environment and are potential agents of eye pathologies.
10
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Content available

The most common eye disease in cat

80%
Trbolova A.
Okulistyka Weterynaryjna. e-kwartalnik dla lekarzy i studentów weterynarii
|
2011
|
nr 2
Wiele chorób oczu kotów różni się od tych które spotykamy u inny gatunków zwierząt. Typowo kocim schorzeniem jest sequestrum czyli martwak rogówki. Ogólne objawy ze strony oczu kota są zwykle powiązane z istnieniem choroby systemowej, toczącej się w całym organizmie (choroby tła wirusowego, pasożytnicze, grzybice, nadciśnienie). Oczy kotów wrażliwe są na niedobory tauryny  czy też tiaminy, które to powodują zmiany chorobowe dna oka prowadzące do ślepoty. Bardzo często spotykamy u kotów nowotwory, które są zwykle bardziej agresywne niż u innych gatunków zwierząt. Ponadto występują też schorzenia o podłożu genetycznym i wady rozwojowe.
11

Isolation and identification of Acanthamoeba spp. from thermal swimming pools and spas in Southern Brazil

80%
Fabres L. F., Santos S. P. R., Benitez L. B., Rott M. B.
Acta Parasitologica
|
2016
|
tom 61
|
nr 2
12

Virulent T4 Acanthamoeba causing keratitis in a patient after swimming while wearing contact lenses in Southern Brazil

80%
Fabres L. F., Maschio V. J., Santos D. L., Kwitko S., Marinho D. R., Araujo B. S., Locatelli C. I., Rotta M. B.
Acta Parasitologica
|
2018
|
tom 63
|
nr 2
13

Isolation of Acanthamoeba isolates belonging to T2, T3, T4 and T7 genotypes from environmental samples in Ankara, Turkey

80%
Kilic A., Tanyuksel M., Sissons J., Jayasekera S., Khan N. A.
Acta Parasitologica
|
2004
|
tom 49
|
nr 3
Acanthamoeba keratitis is a blinding infection that is becoming increasingly important in human health. Early diagnosis is a prerequisite for successful treatment and requires identification of Acanthamoeba at the genotypic level. The genus Acanthamoeba consists of both pathogenic and non-pathogenic species and has been recently classified into 13 different genotypes, T1-T12 and T14. More importantly, 95% of Acanthamoeba isolates that produce keratitis belong to T4 genotypes. In this study, we attempted to determine whether predominance of T4 isolates in Acanthamoeba keratitis is due to greater virulence or greater prevalence. We isolated 18 Acanthamoeba isolates from environmental samples in Ankara, Turkey and determined their pathogenic potential by means osmotolerance, temperature tolerance and in vitro cytotoxicity assays using corneal epithelial cells. Ribosomal DNA sequencing revealed that 10 isolates belong to T2, 5 belong to T3,2 belong to T4 and one belongs to T7 genotype. As expected, T3 and T4 isolates exhibited the most pathogenic traits and were osmotolerant, temperature tolerant and exhibited severe corneal epithelial cell cytotoxicity indicating their pathogenic potential. Overall these data indicate that high frequency of T4 isolates in keratitis cases may well be due to their greater virulence. This is the first report presenting environmental distribution of Acanthamoeba in Ankara, Turkey.
14

Uszkodzenia rogówki u koni: diagnostyka i leczenie

51%
Zak A., Siwinska N.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2017
|
tom 12
|
nr 1
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