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The study was to demonstrate the effect of sewage sludge open-air drying on the quantitative and qualitative composition of keratinolytic/keratinophilic and actidione-resistant fungi. The sludge was being dried for up to thirty days (on average fourteen days) at 25-30°C. The composition of these fungi was determined with the hair baiting method along with the dilution method, using the Wiegand medium supplemented with chloramphenicol (100 mg/L) and actidione (500 mg/L). The open- air drying altered the composition of keratinolytic fungi and considerably increased the population of keratinophilic and actidione-resistant fungi in the sludge. This phenomenon can be explained with that the drying process was associated with slow sludge moisture decrease, sludge laceration due to crumbling and the subsequent improvement of sludge aeration and organic matter biodegradation conditions. A considerable increase of fungal populations can be expected in sludges being dried in drying beds at wastewater treatment plants and in sludge-amended soils. Two sludge opportunistic fungi, i.e. Microsporum gypseum and Pseudallescheria boydii, require special attention from the epidemiological point of view. Sludge land applications may increase the number of these fungi in the environment and the subsequent risk to public health posed by them.
Molecular analysis of dermatophytes (based on PCR fingerprinting) revealed high clonal differentiation between the genus and species. Microsporum canis (zoophilic dermatophyte, belonging to genus Microsporum), responsible for most cases of tinea capitis in children, tinea corporis in adults and dermatophytoses in cats, is very unique in comparison with other dermatophytes. Results of most molecular studies show that there is no clonal differentiation within M. canis as distinct from other species. The aim of this study was application of (GACA)4 repetitive primer and (ACA)5 primer for typing of M. canis strains isolated from human and animals in Central Poland. Fungal strains: 32 clinical isolates of M. canis, originated from patients from Central Poland; 11 strains isolated from infected cats (6) and dogs (7), reference strains of M. canis (CBS 113480), T. rubrum (CBS 120358), T. mentagrophytes (CBS 120357) and E. floccosum (CBS 970.95). The genomic DNAs of the strains were used as a template in RAPD reaction. No differentiation was observed for the analyzed M. canis strains using (GACA)4 and (ACA)5 typing.
This study aimed to determine the influence of sewage sludge alkalization and acidification on the qualitative and quantitative composition of keratinolytic and keratinophilic fungi in a model experiment. The sludge was alkalized to pH 9 with 0.5N NaOH, 0.5N KOH, 0.5N NH₄OH or with 2% burnt lime mixed with powdered limestone (20g CaO + 5g CaCO₃), and acidified to pH 4 with 0.5N HCl, 0.5N HNO₃ or 0.5N H₂SO₄. The sludge with unmodified pH (6.5) served as control. The hair baiting method with four incubation temperatures (23, 29, 33, and 37°C) was used to examine fungi. The sludge alkalization with KOH, NaOH or CaO+CaCO₃ increased the number of keratinolytic fungi but decreased the number of keratinophilic fungi. The inhibition of the growth of keratinolytic fungi was observed due to the sludge acidification with H₂SO₄ or HCl. The sludge acidification with HNO₃ or HCl stimulated keratinophilic fungi to grow. Sludge acidification with HNO₃ eliminated keratinolytic fungi, while acidification with H₂SO₄ eliminated keratinophilic fungi. Results are discussed from ecological and epidemiological points of view.
Due to the content of pathogenic organisms, including fungi, sewage sludge land application poses risks to both human health and the environment. One of the ways for reducing pathogens in sludge is liming. This study aims to determine the effect of sludge liming on the composition of keratinolytic and keratinophilic fungi in model experiments. The fungi were examined using the hair baiting method and the dilution method with incubation on a Wiegand medium supplemented with chloramphenicol (100 mg/L) and actidione (500 mg/L). The sludge liming considerably decreased the number of actidione-resistant fungi propagules and eliminated many fungal species, including Pseudallescheria boydii. The influence of this process on hair-baited fungi was that the liming eliminated keratinolytic and keratinophilic fungi at 37°C. In the range of 23-33°C, the liming considerably restricted the growth of keratinolytic fungi, including Microsporum gypseum, but only slightly affected keratinophilic fungi, including Pseudallescheria boydii. The sludge liming decreases the risk posed by geophilic dermatophytes and other keratinolytic fungi, as well as by keratinophilic fungi to humans and the environment. The process affected more keratinolytic fungi than keratinophilic ones.
Sewage sludge was examined for keratinolytic and keratinophilic fungi by means of a multi-tempera-ture (23, 26, 29, 33, 37 and 45oC) hair baiting method. Results indicate that this method can be a useful tool for determination of the influence of temperature and other factors on fungal composition in sludge and evaluation of health risk posed by fungal pathogens. The use of the method facilitated recognition of fungal flora much more than a traditional room-temperature method. The method was adapted to quantitative pur-poses. The effect of Petri dish sludge weight on fungal composition was determined. A 10 g weight should be used in qualitative studies while larger weights are more suitable for determination of the influence of environmental factors on fungal growth and composition.
Interactions between selected geophilic fungi (keratinofytic and keratinophylic species) and pathogenic dermatophytes were examined in dual cultures on Sabouraud glucose agar (SGA). It was demonstrated that Trichophyton mentagrophytes var. mentagrophytes together with T. mentagrophytes var., quinceanum are the species most sensitive to the influences of the geophilic fungi. Microsporum persicolor was found to be more resistant to these influences. Only a few geophilic species inhibited the growth of M. canis on SGA. Keratinophilic species, unable to initiate hair decomposition but utilize the products of this process, exerted the larger inhibitory effect on the pathogenic dermatophytes than keratinofytic fungi.
The mycoflora developing on the feathers of wild and domestic bird species in the water of 6 limnologically different water bodies was investigated under laboratory conditions. 97 zoosporic fungus species were found to grow on the feathers investigated, including 21 Chytridiomycetes, 1 Hyphochytriomycetes, 74 Oomycetes and 1 Zygomycetes fungus. The most common fungus species included Chytriomycetes annulatus, Rhizophydium keratinophilum, Blastocladiopsis parva, Catenaria anguillulae, Catenophlyctis variabilis, Aphanomyces helicoides, Aphanomyces irregularis, Leptolegniella piligena, Pythium afertile, Pythium aquatile, Pythium echinulatum, Pythium intermedium and Pythium tenue. The most fungi were noted growing in water from Cypisek spring (64), the fewest in the ponds Akcent (45) and Fosa (47 species). Out of these 97 species, 17 are known as parasites or necrotrophs of fish. 13 fungus species were recorded for the first time in Poland.
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