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Kallikrein-thrombolytic and hypotensive action in cats - preliminary results

100%

Swies J., Grodzinska L., Slawinski M., Gryglewski R. J.

Acta Physiologica Polonica

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1990

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tom 41

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nr 1-3

Święs J., Grodzińska L., Sławiński M., Gryglewski R. J.: Kallikrein-thrombolytic and hypotensive action in cats - preliminary results. Acta Physiol. Pol. 1990, 41 (1-3): 87-95. An intravenous injection of kallikrein produced hypotensive and thrombolytic effects in anasthetized cats, using the blood superfused tendon technique. The thrombolytic action of kallikrein was mediated by an unstable substance. The generation of this substance was abolished by either acetylsalicylic acid (ASA) or aprotonin and enhanced by captopril. The hypotensive action of kallikrein was only partially inhibited by ASA. It is proposed that both these pharmacological effects of kallikrein are mediated by bradykinin which in turn releases prostacyclin from the endothelium. However, in contrast to the thrombolytic effect of kallikrein which is totally mediated by prostacyclin the hypotensive action of kallikrein depends not only on prostacycylin but also on another endothelium-derived vasorelaxant, e.g. EDRF.

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Properties of chemically oxidized kininogens

100%

Niziolek M., Kot M., Pyka K., Mak P., Kozik A.

Acta Biochimica Polonica

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2003

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tom 50

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nr 3

Kininogens are multifunctional proteins involved in a variety of regulatory processes including the kinin-formation cascade, blood coagulation, fibrynolysis, inhibition of cysteine proteinases etc. A working hypothesis of this work was that the properties of kininogens may be altered by oxidation of their methionine residues by reactive oxygen species that are released at the inflammatory foci during phagocytosis of pathogen particles by recruited neutrophil cells. Two methionine-specific oxidizing reagents, .-chlorosuccinimide (NCS) and chloramine-T (CT), were used to oxidize the high molecular mass (HK) and low molecular mass (LK) forms of human kininogen. A nearly complete conversion of methionine residues to methionine sulfoxide residues in the modified proteins was determined by amino acid analysis. Production of kinins from oxidized kininogens by plasma and tissue kallikreins was significantly lower (by at least 70%) than that from native kininogens. This quenching effect on kinin release could primarily be assigned to the modification of the critical Met-361 residue adjacent to the internal kinin sequence in kininogen. However, virtually no kinin could be formed by human plasma kallikrein from NCS-modified HK. This observation suggests involvement of other structural effects detrimental for kinin production. Indeed, NCS-oxidized HK was unable to bind (pre)kallikrein, probably due to the modification of methionine and/or tryptophan residues at the region on the kininogen molecule responsible for the (pro)enzyme binding. Tests on papain inhibition by native and oxidized kininogens indicated that the inhibitory activity of kininogens against cysteine proteinases is essentially insensitive to oxidation.

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Long-term feeding with bioactive tripeptides in aged hypertensive and normotensive rats: special focus on blood pressure and bradykinin-induced vascular reactivity

67%

Siltari A., Roivanen J., Korpela R., Vapaatalo H.

Journal of Physiology and Pharmacology

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2017

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tom 68

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nr 3

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Kinins and thrombolysis

67%

Swies J., Chlopicki S., Gryglewski R. J.

Journal of Physiology and Pharmacology

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1993

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tom 44

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nr 2

Ograniczanie wyników

2 Journal of Physiology and Pharmacology

1 Acta Biochimica Polonica

1 Acta Physiologica Polonica

2 Gryglewski R. J.

2 Swies J.

1 Chlopicki S.

1 Grodzinska L.

1 Korpela R.

1 Kot M.

1 Kozik A.

1 Mak P.

1 Niziolek M.

1 Pyka K.

1 Roivanen J.

1 Siltari A.

1 Slawinski M.

1 Vapaatalo H.

1 2017

1 2003

1 1993

1 1990

Kallikrein-thrombolytic and hypotensive action in cats - preliminary results

Properties of chemically oxidized kininogens

Long-term feeding with bioactive tripeptides in aged hypertensive and normotensive rats: special focus on blood pressure and bradykinin-induced vascular reactivity

Kinins and thrombolysis