Ograniczanie wyników

Czasopisma help
Autorzy help
Lata help
Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 56

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  izolaty grzybowe
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
Relationship between activity of hydrolases in fungi isolated from vagina of pregnant women and selected symptoms. Pregnancy is considered a factor of vulvovaginal mycosis. Secretion of hydrolases is an important determinant of Candida virulence. Thus, the aim of the study was to found the relationsbip between activity of 19 hydrolases in fungi isolated from vagina of pregnant women and symptoms of mycosis. 251 pregnant women were examined. Samples were collected from vagina and cultured on Sabouraud media. Activity of hydrolytic enzymes was evaluated using API ZYM (bioMerieux) test. Fungi were found in 20.1% of vaginal samples. Symptoms were detected in 45.8% of wornen. Only 32.1% of women with discharge and 26.4% with pruritus had mycosis. Out of the 19 examined hydrolazes, 13 active enzymes were detected in fungal strains. We found for the first time the relationsbip between activity of fungal esterase lipase and the presence of vaginal discharge in pregnant women.
We have evalueated the activity of acidous asparagine protease in 48 strains of Candida isolated from homogenates of palatine tonsils from 116 patients after tonsilectomy. Strains most frequently isolated were C. albicans (66.7±6.8 %) and C. tropicalis (14.6±5.09 %). The range of proteolysis zone for Candida strains was 4.6 to 12.5 mm. Over 60% of the studied strains showed high and very high proteolytic activity (>6.6 mm) and the remaining strains with showed moderate activity (4.6-6.5). There were no strains with low medium activity (to 4.5 mm zone). The range of the number of rosettes E formed by lymphocytes obtained from the studied tonsils was 0-268.3. Lymphocytes isolated from 70 % of tonsils infected with Candida formed only 0-130 rosettes E; in the remaining cases the number ranged between 131 and 220. The number of lymphocytes T forming rosettes E isolated from palatine tonsils from patients not infected with fungi was 2.5 times higher. There was no correlation between the size of proteolysis zone and the number of rosettes E.
We have investigated the hydrolytic activity of 21 species of yeast-like fungi from Candida genus and l species of yeast S. cerevisiae isolated from Sulejów Reservoir water. The activity of hydrolytic enzymes of the studied fungi was different than that of standard strains and also of fungi isolated from patients. This difference demonstrates the capability of the studied fungi to adapt to various environmental conditions.
Fermentation of carbohydrates and assimilation of carbon compounds were evaluation. The utilization of 18 specific carbon compounds was estimated in the investigation of each strains for differentiation of the species. From among 125 strains of fungi the following were found: Candida albicans ( 112 strain), C. famata (2), C. glabrata (1). C. guilliermondii (1), C. kefir (4), C. tropicalis (2). The activity of 19 hydrolases was investigated using API ZYM. Biotyping of Candida strains was done according to the Williamson classification ( 1986), modified by Kurnatowska (1998). All strains were isolated from the oral cavity, rectum, as well as from the materials collected during endoscopy in children.
16
84%
The aim of the work was analyzing of genomic DNA of Malassezia pachydermatis isolates from clinical cases otitis externa from dogs using RAPD method with arbitrary primers Eric1R, Eric2, BG2 and FM1. Materials and methods. 47 strains of M. pachydermatis isolates from clinical cases otitis externa from dogs were tested. Isolation of genomic DNA was provided according with MasterPureTM Yeast DNA Purification Kit EPICENTRE procedure. The quality of isolated genomic DNA was determined electrophoreticaly. For differentiation the following primers were used: Eric1R, Eric2, BG2 and FM1. Primers Eric 1R and Eric 2 were used together in one reaction or amplificated separately. Obtained products were analyzed electrophoreticaly in 1.5% agarose gel. For determination of phylogenic tree Quantity one VersaDoc (BioRad) and Statgraphics plus 4.1 programs were used. Results. High degree of heterogeneity of DNA among investigated isolates of M. pachydermatis was shown using FM1 primer. Dendrograms were prepared by calculation euclid's distance of different parameters (size and count of RAPD products) by nearest neighbor method. Basing on phylogenic tree four main types (phylogenic groups) of M. pachydermatis isolates were shown. The other five groups non-count was shown also.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.