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Composition of sterol fraction and content of identified compounds in herb of fireweed (Chamaenerion angustifolium fU Scop.) and leaves of evening primrose (Oenothera biennis L.) obtained from several populations of these species growing wild in Poland were determined by HPLC. The investigated species differed in respect of the composition and content of identified sterols. High intraspecific variability concerning accumulation of sterols was also observed. Herb of fireweed was characterised by relatively high content of p-sitosterol (85.80-171.18 mg/100 g), campesterol (24.24-334.49 mg/100 g), and p-sitosterol D-glucoside (26.33-86.32 mg/100 g). Leaves of evening primrose appeared to be much poorer source of sterols. Content of |3-sitosterol (the dominant compound in the sterol fraction) in this raw material ranged from 5.21 to 34.66 mg/100 g.
Acta Agrobotanica
|
1995
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tom 48
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nr 1
45-51
The study on wild oat variability was carried in 1986-89y. From among plants collected on cultivated fields were separated 10 species, 4 known in Poland and 6 new. Those had different highness of plants, length of panicles, pubescens and colour of kernels, callus pubescens and mass of 1000 grains. Avena fatua var. fatua and generally species with long callus pubescens were most numerous represented in investigated region. Species with short pubescens of callus were on the average higher, with longer panicles and bigger mass of 1000 grains.
The content and composition of sterol compounds in wild growing great burnet (Sanguisorba officinalis L.) populations were determined. The herb and underground organs of this plant were collected from 10 natural sites in Poland. The HPLC analysis revealed the presence of 5 sterol compounds, namely β-sitosterol, β-sitosterol D-glucoside, campesterol, brassicasterol and stigmasterol in these raw materials. Among them, β-sitosterol Dglucoside was dominant one. The herb was richer in sterol compounds than underground organs. There were significant differences between the content of above mentioned compounds in these populations.
Pollen morphology and pollen variability of Rubus gracilis were studied. A total of 260 grains from 13 natural Polish localities were examined. Important characteristics include: a stretched bridge; a visible pore area and endopores; ectocolpi arranged regularly, more or less evenly spaced or, more rarely, joining one another in the apocolpium, long (79.3% length of the polar axis) and narrow; exine sculpture striate, distinct; striae and muri of equal width, rather narrow; striae usually running parallel to the polar axis, sometimes forming semicircles in the apocolpium zone; with distinct perforations. The size, outline and shape turned out to be poor criteria when identifying the species. A statistical analysis of 10 quantitative grain characteristics showed their little variability. The highest variability was found to occur in two traits connected with d (the distance between the apices of two ectocolpi). Statistical studies revealed no differences among the grains from the individual localities, likely the result of apomixis.
Inter- and intrapopulational morphological variability of Elymus repens, a cool-season peren-nial grass and a tenacious weed that spreads both by seeds and rhizomes forming a thick and stout net, was examined. Multivariate statistical analysis of 48 initial morphological characters obtained from 44 population samples (1180 specimens) collected in different habitats revealed the conspicuous variation within E. repens. Principal Component Analysis revealed three extremes of the morphological gradient that were referred to as three varieties of E. repens: var. repens, var. aristatus and var. subulatus, not correlated with the habitat type or geographi-cal location. Canonical Discriminant Analysis proved the taxonomic usefulness of characters such as awned or awnless glumes and lemmas as well as the shape of glumes for variety di-stinction. Results of morphological analysis, in relation to the low level of genetic variation showed in the previous studies, suggest that the extensive morphological variation of E. re-pens can be caused by its plasticity where different morphological patterns are realized on the same genome basis. Additionally, the open-pollinated system of mating results in new combi-nations of morphological characters of plants that further reproduce vegetatively by rhizomes. The nomenclature, taxonomic descriptions and a key to the three varieties are provided.
The chemical composition of the herb of four populations of common burstwort (Herniaria glabra L.) growing wild in the middle part of the Bug river valley was compared. The investigated populations differed significantly as to the content of saponins (3.47-5.29 g/100 g), tannins (0.75- 1.40 g/100 g) and flavonoids (620.7-1071.9 mg/100 g), but only slightly in the content of polyphenolic acids (0.64-0.84 g/100 g). The predominant phenolic acids turned out to be pyrocatechuic and p- hydroxybenzoic acids, and the main flavonoid compounds were isorhamnetin 3-rhamnoglucoside and rutin.
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