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Fish of five species of Notothenioidei (104 specimens), Cottoperca trigloides, Patagonotothen brevicauda, P. longipes, P. tessellata and Champsocephalus esox, caught in the Beagle Channel (Magellanic sub-region, sub-Antarctica) were infected with Digenea of nine species (1130 specimens). Faunistic data on the occurrence of all nine parasites are provided. The most abundant digenean species was Macvicaria magellanica found in the intestine of three host species of the genus Patagonotothen. The second most abundant digenean species was Elytrophalloides oatesi found in the stomach of four host species, with exception of P. brevicauda. Three digenean species: Stenakron kerguelense, Whitegonimus ozoufae and Genolinea bowersi, were more abundant in fish caught at the harbor of Ushuaia (depth 7–9 m), remaining six species: M. magellanica, Neolepidapedoides subantarcticus, Postmonorchis variabilis, Derogenes varicus, E. oatesi and Lecithaster macrocotyle, in the eastern mouth of the Beagle Channel (depth 30 m).
Background. The ability of lactobacilli to adhere to the surface of the intestine is an important functional characteristic which can largely determine the effective colonization of the intestinal tract by probiotics. The following study compares the adhesion efficiency of the twenty strains of Lactobacillus genus belonging to Casei group to the Caco-2 cells and gastrointestinal mucus. Material and methods. Twenty isolates of lactobacilli belonging to Casei group were tested. The ability of bacterial cells to adhere to mucus was examined using adhesion assay to gastrointestinal mucus. Obtained results were compared with adhesion efficiency to Caco-2 cells. Phylogenetic relationship between isolates was analysed by rep-PCR. Results. The results showed large differences in adhesion efficiency between strains, as well as differences in the efficiency of adhesion to the intestinal epithelial cells and mucus. Group similarity highlighted by a rep- PCR technique does not correspond with groups of similarity in terms of the characteristics of the ability to adhere to mucus or the epithelial cells of intestinal tract. Conclusions. Strains having a high adhesion efficiency to enterocytes do not always show a high adhesion efficiency to the mucus. This may indicate the presence of different and multiple factors responsible for adhesion efficiency of Lactobacillus group Casei strains to epithelial cells and mucus.
Rozwojowi przedwczesnych chorób układu krążenia, takich jak zawał mięśnia sercowego, często towarzyszą zaburzenia w metabolizmie lipidów, szczególnie hipertrójglicerydemia i dyslipoproteinemia. Praca ta zaznajamia ze stosowaną nomenklaturą i omawia kolejne etapy w metabolizmie lipidów i lipoprotein w zdrowiu i chorobie.
We investigated wolf Canis lupus Linnaeus, 1758 food habits in central Italy by examining stomach and/or intestine contents of 59 individuals. Road accident and illegal kills were main causes of the wolves' death. Ungulates represented the bulk of the diet (55% in frequency), and among them wild boar was the most important prey, followed by domestic Caprinae. Food items of domestic origin accounted for about 1/3 of all the diet. Diet composition did not vary between stomachs and intestines in spite of the higher degree of digestion of the intestines' contents. The frequent detection of numerous larvae of Diptera and/or necrophagous Coleoptera, let suppose the consumption of already dead animals, and suggests a general underestimate of the wolfs scavenging behaviour in previous studies based on scat analyses.
In order to examine the relation of known intestinal lesions to changes in T-cell phenotypes and integrin expression, 16 male 10-day-old Holstein calves were divided into two groups. For 28 days of the experiment, eight males were fed NutriMilk in which 50% of the crude protein was soya protein, and eight control animals, with NutriMilk containing only milk casein. The animals fed soya protein showed shorter jejunal villi with a corrugated surface and deeper crypts compared with the control calves. A higher density of CD8+ cells in the intestinal mucosa and a decrease of these cells in peripheral blood were found in calves fed soya protein. The number of CD11b-positive cells was decreased in the peripheral blood of calves fed soya protein. Lower expression of integrin could be related to the appearance of non-mature polymorphonuclear cells. It is not clear if the decrease in CD11b expression on blood cells could also be influenced by milk replacer, i.e. soya protein.
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Ontogeny regulates creatine metabolism in rat small and large intestine

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The ontogeny of intestinal CRT, AGAT and GAMT was investigated in foetuses, newborn, suckling, weaning and adult rats. In the colon, CRT mediates creatine transport because it was Na+- and Cl- dependent and inhibited by creatine and GPA. In addition, Northern assays showed two CRT transcripts (2.7-kb and 4.2-kb) and the in situ hybridisation revealed that CRT mRNA is restricted to the colon epithelial cells. The immunohistochemistry revealed that CRT protein was at the apical membrane of colon epithelia. Maturation decreased colonic CRT activity to undetectable levels and increased CRT mRNA abundance. Western assays revealed 57-, 65-, 80- and 116-kDa polypeptides at the intestinal apical membrane. The abundance of the 65-, 80- and 116-kDa polypeptides decreased with age, and that of 57-kDa was only observed in adult rats. The small and large intestine express AGAT and GAMT mRNAs. Maturation decreased AGAT mRNA abundance without affecting that of GAMT. For comparison, renal AGAT mRNA levels were measured and they were increased with age. The study reports for the first time that: i) the apical membrane of rat colon have an active CRT, ii) development down-regulates CRT activity via post-transcriptional mechanism(s), iii) the intestine might synthesize creatine and iv) intestinal and renal creatine synthesis is ontogenically regulated at the level of AGAT gene expression.
 Introduction: Hydrogen breath test (BT) is commonly used as a diagnostic tool for the detection of small intestine bacterial overgrowth (SIBO). It was reported that colonic methane production is far more frequent in cystic fibrosis (CF) patients than in other subjects. Therefore, measuring exclusively hydrogen in the diagnostic breath test for diagnosing SIBO might be of limited value. We aimed to assess the usefulness of combined measurement of hydrogen and methane expiration for the diagnosis of SIBO in CF. Material and Methods: The study comprised 62 CF patients aged 5 to 18 years. Three-hundred-ninety subjects assessed due to gastrointestinal symptoms for the presence of SIBO served as a comparative group. In all subjects hydrogen/methane BT using glucose was performed. A positive BT was defined as fasting hydrogen ≥ 20 ppm or fasting methane ≥ 10 ppm or a rise of ≥ 12 ppm hydrogen or ≥ 6 ppm methane over baseline during the test. Results: In 23 (37.1%) CF patients and in 52 (13.3%) subjects from the comparative group abnormal BT results were found. In seven (11.3%) CF patients and 29 (7.4%) of the other subjects studied methane measurement allowed diagnosis of SIBO. Conclusions: Small intestine bacterial overgrowth is frequent in cystic fibrosis. For its detection in cystic fibrosis and other gastrointestinal patients, combined hydrogen and methane measurement instead of hydrogen breath test should be applied. Without the additional measurement of methane a significant percentage of SIBO will be missed.
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