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The diet of the Saimaa ringed seal Phoca hispida saimensis Nordquist, 1899 was studied by the analysis of stomach contents and by feeding trials with a captive seal. Nine prey species were found in the stomachs, the most important being small schooling fish species: perch Perca fluviatilis, roach Rutilus rutilus, vendace Coregonus albula, smelt Osmerus eperlanus and ruff Acerina cernua, The importance of crustaceans in the diet of the ringed seal in Lake Saimaa is insignificant. Length of the intestinal tract of the Saimaa ringed seal is relatively shorter than those of marine ringed seals. In cafeteria tests on one captive seal the preferred fish species were vendace and smelt. The captive seat displayed clear seasonal variation in feeding activity. The consumption of fish was lowest in springtime and highest in autumn and winter.
Tea infusions are consumed frequently and in large amounts, so they may play a role in modelling intestinal microflora or in preventing bacterial diseases. The present study examined the effect of different kinds of tea (black, green and puerh) on Lactobacillus rhamnosus, Lactobacillus plantarum, Leuconostoc mesenteroides subsp. mesenteroides, Escherichia coli, Staphylococcus aureus and Salmonella enteritidis. In order to ascertain the connection between antioxidant and antimicrobial properties, the content of polyphenolic compounds and the reducing power of the teas were assessed. The rate of bacterial proliferation was measured by calculating the generation time between the 2nd and 6th hour of incubation. Staphylococcus aureus was the most sensitive to the addition of tea extracts to the media. Among lactobacilli (LAB), Lactobacillus casei proved to be sensitive mainly to the extracts of black and puerh tea. The value for reducing power does not fully correspond to the content of polyphenols and antimicrobial properties.
The intestinal and total tract digestibilities of phosphorus (P) and phytate P were determined in digestibility trials with laying hens and broiler breeders fed a diet containing P, phytate P and Ca at 6.37, 2.00 and 34.1 g·kg-1, respectively. In both digestibility trials, the total tract digestibility of phytate P was higher than the intestinal digestibility (33 and 35% vs 20 and 18%). In contrast, in both trials the total tract retention of P was lower than the intestinal retention (22 and 19% vs 52 and 42%). Phytate P represented 29.2% of the excreta P of laying hens and 23.6% of the excreta P of broiler breeders. The corresponding proportions of phosphate P were 48.7 and 46.6%, respectively. Samples of digesta and small intestinal mucosa of laying hens were diluted with physiological saline containing sodium phytate and incubated in vitro to determine the phytase activity. The average specific phytase activities in the crop, stomach, small intestinal contents, mucosa and caecal contents were 10.2, 9.2, 14.6, 11.5 and 135 μmol·h-1·g-1, respectively. The total phytase activities (per segment) in the small intestine (including the mucosa) and the caeca were 586 and 663 μmol·h-1, respectively. It can be concluded that phytase activity was present in all sections of the digestive tract. Consequently, in hens fed a wheat-maize-soyabean diet without a phytase supplement, phytate P was partially digestible. A part of the phytate degradation occurred in the hindgut.
An undescribed species of Microsporidia Balbiani, 1882 was isolated from the muscularis mucosa of the intestinal mucosa of the reared Atlantic bluefin tuna (Thunnus thynnus). Transmission electron microscopy showed that the pyriform unikaryotic microsporidium, measuring 3.6 μm ± 0.08 in length and 1.8 μm ± 0.04 in width (inferred from TEM sections), had two layers of 14–17 coils of polar filament and a robust manubrium of the filament. No developmental stages were observed in xenoma. Phylogenetic analysis of the small subunit rRNA showed closest similarity with Kabatana spp. (88.4%), clustering the microsporidium together in a sister group with K. takedai and Kabatana sp. (JI-2008)/K. newberryi clade. While recognized Kabatana members show ovoid, rounded to pyriform spore, lack of sporophorous vesicle or xenoma, 3–10 coils of polar filament in 1–2 rows and are localized within cytoplasm of trunk muscle, the new species is markedly pyriform, xenoma forming, with many filament coils in 2 rows and parasitizing smooth intestinal muscle. Since morphological features were not typically congruent with any of Kabatana spp. so far described and molecular clustering indicated paraphyletic position within Kabatana clade, we suggest assigning described species to collective group Microsporidia, as Microsporidium milevae sp. nov., until more evidence permits potential formation of the new genus.
The aim of this study was to investigate the effect of two levels of whey protein concentrate (WPC, 80.35% protein, 5.86% lactose) added to diets and duration of these treatments on growth and slaughter value, haematological parameters involved in immune processes, oxidative status of liver and breast muscle, and selected indices of gut function of broilers. A total of 560 Ross 308 broilers were randomly allocated into 7 groups with 10 replicates of 8 chickens per replicate. The experiment lasted 42 days and was divided into 3 successive feeding phases. The control group was fed basal diets consisted of maize, wheat and soyabean meal (C group). The other 6 groups received, for 7, 21 and 42 days basal diets with 8 or 32 g · kg–1 WPC added at the expense of soyabean meal (WL and WH groups, respectively). On day 42 group WH had a higher lymphocyte percentage (P < 0.05) compared to the control. The long-term (42 days) feeding with higher level of WPC significantly decreased the heterophile/lymphocyte ratio (P < 0.05 vs C). The number of erythrocytes on day 42 and the whole blood glucose on day 21 were significantly increased in WH birds than in controls. The concentration of serum total protein on both WPC levels was greater (P < 0.05) on day 21 compared with the C group. The reduction (P < 0.05) in liver thiobarbituric acid reactive substances (TBARS) and a tendency towards lower TBARS level in breast meat were observed in birds WH when fed for 42 days. Only higher dietary WPC amount provided for 42 days reduced the caecal and small intestinal pH values (P < 0.05 vs C). The ileal digestibility of crude protein was higher (P < 0.01) in birds fed with 32 g WPC during the first 21 days of life, compared to the C and WL treatments. The factorial ANOVA showed that on days 7, 21 and 42 the final body weight gain (BGW) and feed conversion ratio in broilers WL and WH were significantly better than in the C ones. Assessment of orthogonal contrasts revealed significantly higher (P < 0.05) final BWG in the WH broilers, compared to the WL dietary treatment; such effect was not observed in earlier feeding stages. Generally, carcass yield on both WPC dietary levels, and breast muscle percentage on WH treatment, were higher (P < 0.01 and P < 0.05, respectively) compare to the C group. In conclusion, WPC added to a conventional diets for growing chickens exerts a growth-promoting action and at a dose of 32 g · kg–1 may induce desirable changes in bird’s health and the intestinal tract metabolism.
The relationship between the prevalence of normal serum agglutinins and precipitins to Aeromonas hydrophila and A. veronii biotype sobria in carp and the sensitivity of the fish to infection with these bacteria was studied. The presence of these antibodies depended on carp population and on individual fish. No correlation between the prevalence of agglutinins and the sensitivity of carp to infection was found. On the contrary, the fish having the precipitins to particular Aeromonas strains revealed significantly lower sensitivity to infections with the respective homologous strains than those without the precipitins.
An interdisciplinary research group granted by the German Research Foundation (FOR 438) tested various hypotheses and tired to develop a model for the mode of action of probiotics in pigs. The study included the fields of animal nutrition/digestion physiology, anatomy and histology of the intestinal mucosa, transport and secretory properties of the mucosa, microbiology of the intestinal tract, immune system (classes of intraepithelial lymphocytes, humoral responses), gene expression of the mucosa and finally the in vitro and in vivo resistance against infection with Salmonella. Five trials with ten sows per treatment each and their piglets and two probiotic strains were included in this study. The studied bacterial strains were Enterococcus faecium NCIMB 10415 and Bacillus cereus var. toyoi NCIMB 40112. Concluding from our studies and the published data of others, the effects of probiotics on performance are rarely significant. However, with one exception the incidence of post-weaning diarrhoea under the effect of both probiotics was significantly reduced in the trials of the research group. Furthermore, the identification frequency of various E. coli sero-pathovars relevant in post weaning diarrhoea was reduced in these animals. On the other hand, no significant modifications were found for the morphology and histology of the intestinal mucosa and also not on transport properties of this tissue. A further important finding was that the mode of action for probiotics is not unique but species or even strain specific. Most probably the studied probiotics act directly and/ or via modifications of the intestinal microbiota on the immune system (intraepithelial lymphocyte population).
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