Wyniki wyszukiwania

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Trehalase as a possible marker of intestinal ischemia - reperfusion injury

100%

Toth,Jr. S., Pekarova T., Varga J., Tomeckova V., Toth S., Lakyova L., Vesela J.

Acta Biochimica Polonica

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2013

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tom 60

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nr 3

Background: Different pathological affections of the small intestine cause corresponding morphological and functional changes. The present study was aimed to assess intestinal trehalase activities during ischemia and following reperfusion, correlate them with the pathological changes and determine whether trehalase could be used as a biochemical marker of the intestinal ischemia, ischemia - reperfusion injury. Material and methods: Wistar rats, randomly divided into 5 experimental groups (IR) (each n=15), were subjected to one hour mesenteric ischemia followed by 0, 1, 4, 12 and 24 hours of reperfusion. As a control group sham operated animals were used (n=15). The activity of trehalase was determined using an adapted Dahlqwist method. The range of intestinal injury was determined using histological (histopathological injury index and goblet cell quantification) and immunohistochemical (Ki67, InSitu TUNEL) methods. Results: The highest activities of trehalase were recorded in the control group (C=4.42±0.373 μmol/mg/h). The most altered intestinal histology detected in group IR1 was accompanied by the lowest trehalase activity (IR1=0.97±0.209 μmol/mg/h; p < 0.001 C vs. IR1). Improved histological structure in the remaining reperfusion periods correlated with increase in trehalase activity. Almost normal mucosal histological architecture and 72% of the enzymatic activity were restored after 24 hours of reperfusion (IR24=3.20±0.266 μmol/mg/h; p < 0.01 IR1 vs. IR24). Conclusion: The correlation between intestinal histology and trehalase activities during intestinal injury has been shown. Trehalase activity is closely associated with the status of the histological architecture of the small intestine.

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Vulnerability of gastric and small intestinal mucosa to ulcerogenic action of indomethacin in C57/BL6/J mice and transient receptor potential channel vanilloid type 1 knockout mice

84%

Yarushkina N. I., Sudalina M. N., Punin Y. M., Filaretova L. P.

Journal of Physiology and Pharmacology

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2018

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tom 69

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nr 6

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Indomethacin-induced generation of reactive oxygen species leads to epithelial cell injury before the formation of intestinal lesions in mice

67%

Tomita T., Sadakata H., Tamura M., Matsui H.

Journal of Physiology and Pharmacology

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2014

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tom 65

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nr 3

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Aspirin’s ability to induce intestinal injury in rats is dependent on bile and can be reversed if pre-associated with phosphatidylcholine

67%

Lichtenberger L. M., Phan T., Okabe S.

Journal of Physiology and Pharmacology

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2011

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tom 62

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nr 4

Ograniczanie wyników

3 Journal of Physiology and Pharmacology

1 Acta Biochimica Polonica

1 Filaretova L. P.

1 Lakyova L.

1 Lichtenberger L. M.

1 Matsui H.

1 Okabe S.

1 Pekarova T.

1 Phan T.

1 Punin Y. M.

1 Sadakata H.

1 Sudalina M. N.

1 Tamura M.

1 Tomeckova V.

1 Tomita T.

1 Toth S.

1 Toth,Jr. S.

1 Varga J.

1 Vesela J.

1 Yarushkina N. I.

1 2018

1 2014

1 2013

1 2011

Trehalase as a possible marker of intestinal ischemia - reperfusion injury

Vulnerability of gastric and small intestinal mucosa to ulcerogenic action of indomethacin in C57/BL6/J mice and transient receptor potential channel vanilloid type 1 knockout mice

Indomethacin-induced generation of reactive oxygen species leads to epithelial cell injury before the formation of intestinal lesions in mice

Aspirin’s ability to induce intestinal injury in rats is dependent on bile and can be reversed if pre-associated with phosphatidylcholine