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  1. 19 Acta Parasitologica

  2. 5 Acta Protozoologica

  3. 2 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

  4. 2 Journal of Plant Protection Research

  5. 1 Acta Biologica Cracoviensia. Series Botanica

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  1. 4 Li L.

  2. 3 Zhang L.-P.

  3. 2 Chen H.-X.

  4. 2 Gomez F.

  5. 2 Zhang L.

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  1. 1 2021

  2. 4 2020

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  4. 9 2018

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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Identification of medicinal plant Schisandra chinensis using a potential DNA barcode ITS2

100%
Li X.-K., Wang B., Han R.-C., Zheng Y.-C., Yin H.-B., Xu L., Zhang J.-K., Xu B.-L.
Acta Societatis Botanicorum Poloniae
|
2013
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tom 82
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nr 4
To test whether the internal transcribed spacer 2 (ITS2) region is an effective marker for using in authenticating of the Schisandra chinensis at the species and population levels, separately. And the results showed that the wild populations had higher percentage of individuals that had substitution of C→A at site 86-bp than the cultivated populations. At sites 10-bp, 37-bp, 42-bp and 235-bp, these bases of the Schisandra sphenanthera samples differed from that of S. chinensis. Two species showed higher levels of inter-specific divergence than intra-specific divergence within ITS2 sequences. However, 24 populations did not demonstrate much difference as inter-specific and intra-specific divergences were concerned. Both S. chinensis and S. sphenanthera showed monophyly at species level, yet the samples of different populations shown polyphyly at population level. ITS2 performed well when using BLAST1 method. ITS2 obtained 100% identification success rates at the species level for S. chinensis, with no ambiguous identification at the genus level for ITS2 alone. The ITS2 region could be used to identify S. chinensis and S. sphenanthera in the “Chinese Pharmacopoeia”. And it could also correctly distinguish 100% of species and 100% of genera from the 193 sequences of S. chinensis. Hence, the ITS2 is a powerful and efficient tool for species identification of S. chinensis.
2

Morphological and molecular identification of Paramphistomum epiclitum from buffaloes in Pakistan

86%
Khan I., Afshan K., Shah S., Akhtar S., Komal M., Firasat S.
Acta Parasitologica
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2020
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tom 65
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nr 1
3

Phylogenetic analysis of genetically distinct Enterocytozoon bieneusi infecting renal transplant recipients

86%
Khanduja S., Ghoshal U., Ghoshal U. C.
Acta Parasitologica
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2017
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tom 62
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nr 1
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Screening and identification of novel cellulolytic Trichoderma species from Egyptian habitats

72%
Hewedy O. A., El-Zanaty A. M., Fahmi A. I.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2020
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tom 101
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nr 2
5

Molecular identification of Diphyllobothrium latum and a brief review of diphyllobothriosis in China

72%
Guo A.-J., Liu K., Gong W., Luo X.-N., Yan H.-B., Zhao S.-B., Hu S.-N., Jia W.-Z.
Acta Parasitologica
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2012
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tom 57
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nr 3
Two tapeworm specimens collected in northeast China in 2009 and 2011 were identified as Diphyllobothrium latum based on morphological criteria. Molecular methods were used to confirm their identity and analyze genetic variations compared with published data for this species. Species identity was confirmed by molecular characterization of the 18S rDNA partial sequence, complete sequences of internal transcribed spacers (ITSs) and 5.8S rDNA, and partial sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) and mitochondrial NADH dehydrogenase subunit 5 (nad5). PCR amplification and sequence analysis of 18S rDNA (1472 bp), ITS regions (1218 bp), cox1 (885 bp), and nad5 (1028 bp) revealed that these four sequences showed more than 99% identity to reference sequences for D. latum, confirming that this species is D. latum. To date, a total of 12 diphyllobothriosis cases have been documented in China. This study represents the first molecular characterization of D. latum in China, providing molecular evidence of human diphyllobothriosis in China.
6

First report of molecular identification of Cystoisospora suis in piglets with lethal diarrhea in Japan

72%
Matsubayashi M., Takayama H., Kusumoto M., Murata M., Uchiyama Y., Kaji M., Sasai K., Yamaguchi R., Shibahara T.
Acta Parasitologica
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2016
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tom 61
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nr 2
7

Isolation, identification and characterization of the nematophagous fungus Arthrobotrys (Monacrosporium) sinense from China

72%
Xue Y.-J., Li E.-L., Jing C.-X., Ma L., Cai K.-Z.
Acta Parasitologica
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2018
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tom 63
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nr 2
8

Prevalence and genotypes of Enterocytozoon bieneusi in sika deer in Jilin province, Northeastern China

72%
Zhang X.-X., Cong W., Liu G.-H., Ni X.-T., Ma J.-G., Zheng W.-B., Zhao Q., Zhu X.-Q.
Acta Parasitologica
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2016
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tom 61
|
nr 2
9

Allovahlkampfia minuta nov. sp., (Acrasidae, Heterolobosea, Excavata) a new soil amoeba at the boundary of the acrasid cellular slime moulds

72%
De Obeso Fernadez del Valle A., Maciver S. K.
Acta Protozoologica
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2017
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tom 56
|
nr 3
10

Morphological and molecular study of Neorhadinorhynchus nudus (Harada, 1938) (Acanthocephala: Cavisomidae) from Auxis thazard Lacepede (Perciformes: Scombridae) in the South China Sea

72%
Li L., Chen H.-X., Yang Y.
Acta Parasitologica
|
2018
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tom 63
|
nr 3
11

Phylogenetic analysis of the genus Cylicocyclus (Nematoda: Strongylidae) based on nuclear ribosomal sequence data

72%
Bu Y., Niu H., Zhang L.
Acta Parasitologica
|
2013
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tom 58
|
nr 2
Seven species of Cylicocyclus Ihle, 1922 (Nematoda: Strongylidae) were collected from donkeys from Henan Province, China. Five samples of each species were selected for sequencing. Sixteen different internal transcribed spacer (ITS) sequences representing the seven species of Cylicocyclus were obtained. Sequence differences in the first internal transcribed spacer (ITS-1) among species was lower than that of the second internal transcribed spacer (ITS-2). Phylogenetic analyses were conducted using the combined ITS-1 and ITS-2 data sets from the present study and using reference sequences from the GenBank database. The MP and ML trees were similar in topology. The phylogenetic trees were divided into two clades. Clade I included 8 species of Cylicocyclus; within this group, Cylicocyclus leptostomus (Kotlan, 1920) is nested between different samples of Cylicocyclus ashworthi (LeRoux, 1924), suggesting C. ashworthi may represent a species complex. Clade II included Cylicocyclus elongatus (Looss, 1900) and Cylicocyclus ultrajectinus (Ihle, 1920); however, these two species always clustered with the comparative species (Petrovinema poculatum (Looss, 1900) and Poteriostomum imparidentatum Quiel, 1919), suggesting that C. elongatus and C. ultrajectinus represent members of other genera.
12
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The preliminary stage of SCAR markers development for Ranunculus subgen. Batrachium

72%
Jopek M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2013
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tom 94
|
nr 3
13

A PCR method targeting internal transcribed spacers: the simultaneous detection of Babesia bigemina and Babesia bovis in cattle

72%
Liu J., Guan G., Liu A., Li Y., Yin H., Luo J.
Acta Parasitologica
|
2014
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tom 59
|
nr 1
In this study, two pairs of oligonucleotide primers were designed according to the nucleotide sequence of the internal transcribed spacers (ITSs) of Babesia bigemina and B. bovis isolates from China. The primers were used in a multiplex PCR to detect parasite DNA in blood samples from cattle. There was no cross reactions with B. ovata, B. major, B. sp. Kashi, Theileria annulata, T. sergenti, T. sinensis or normal bovine DNA. The sensitivity of multiplex PCR assay was 1 pg and 10 pg DNA for B. bigemina and B. bovis, respectively. A total of 260 field blood samples collected from cattle in five provinces of China were analyzed by multiplex PCR and light microscopy. PCR testing revealed that 7.3% (19/260) and 5.8% (15/260) of cattle were positive for B. bigemina and B. bovis and 1.2% (3/260) of cattle were co-infected with B. bigemina and B. bovis. Using light microscopy, 2.3% (6/260) and 1.5% (4/260) of cattle were infected by B. bigemina and B. bovis, respectively, and no co-infection was found. The results showed that the multiplex PCR developed in the present study could be an alternative diagnostic tool for the detection of B. bovis and B. bigemina infection in cattle.
14

New haplotypes of Trypanosoma evansi identified in dromedary camels from Algeria

72%
Boutellis A., Bellabidi M., Benaissa M. H., Harrat Z., Brahmi K., Drali R., Kernif T.
Acta Parasitologica
|
2021
|
tom 66
|
nr 1
15
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Problems, limitations, and challenges in species identifcation of Ascomycota members on the basis of ITS regions

72%
Baturo-Ciesniewska A., Pusz W., Patejuk K.
Acta Mycologica
|
2020
|
tom 55
|
nr 1
16

Phylogeny and synonymy of Gyrodinium heterostriatum comb. nov. (Dinophyceae), a common unarmored dinoflagellate in the world oceans

72%
Gomez F., Artigas L. F., Gast R. J.
Acta Protozoologica
|
2020
|
tom 59
|
nr 2
17

Phylogeny and classification of Chinese Bupleurum based on nuclear ribosomal DNA internal transcribed spacer and rps16

72%
Wang Q. Z., Zhou S.-D., Liu T.-Y., Pang Y.-L., Wu Y.-K., He X.-J.
Acta Biologica Cracoviensia. Series Botanica
|
2008
|
tom 50
|
nr 2
105-116
Separate and combined analyses of nuclear ribosomal DNA internal transcribed spacer (ITS) and cpDNA rpsl6 provided phylogenetic hypotheses and molecular data for the taxonomy of Chinese Bupleurum species. The phylogenetic results derived from Bayesian and maximum parsimony analyses supported the monophyly of the Bupleurum with strong evidence. The origin of Chinese Bupleurum is likely to be through the Middle East and the Caucasus from the Mediterranean region with the basic chromosome number (8->7->6), and polyploidization. Our molecular data are not consistent with other earlier Chinese Bupleurum classifications and are consistent with the molecular classification proposed by Neves and Watson. The analyses also provide molecular data to elucidate the taxonomic treatments for Bupleurum falcatum from China and Europe.
18

Differentiation of Toxocara canis and Toxocara cati based on PCR-RFLP analyses of rDNA-ITS and mitochondrial cox1 and nad1 regions

72%
Mikaeili F., Mathis A., Deplazes P., Mirhendi H., Barazesh A., Ebrahimi S., Kia E. B.
Acta Parasitologica
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2017
|
tom 62
|
nr 3
19

A new identification method for Clarias gariepinus, Clarias macrocephalus and their hybrids using PCR-RFLP

72%
Panicz R., Sadowski J., Hofsoe-Oppermann P., Polgesek M.
Electronic Journal of Polish Agricultural Universities. Series Fisheries
|
2018
|
tom 21
|
nr 4
20

Morphological and molecular characterization of Seuratascaris numidica (Seurat, 1917) (Ascaridida: Ascarididae)

72%
Chen H.-X., Zhang K., Zhang L.-P., Li L.
Acta Parasitologica
|
2018
|
tom 63
|
nr 1
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