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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Polymorphism of insulin-like growth factor (IGF-1) gene in Polish Lowland sheep from Podlaskie voivodship

100%
Niznikowski R., Czub G., Kaminski J., Nieradko M., Swiatek M., Glowacz K., Slezak M.
Annals of Warsaw University of Life Sciences - SGGW. Animal Science
|
2014
|
tom 53
Research was carried out on 432 Polish Lowland Sheep (405 ♀ and 27♂) of three varieties: Corriedale, Żelaźnieńska Sheep and Polish Lowland Sheep of Podlasie region. All animals were subjected to gene identifi cation factor insulin – IGF-1, in the assessment of alleles C and T. In conclusion it should be noted that in the three examined varieties of Polish Lowland Sheep showed no polymorphism in exon 3 of the insulin-like growth factor (IGF-1) gene, limiting its scope to determine the allele C, respectively genotype CC. This result indicates the need for further research in this area in “culture” sheep imported and adapted to Polish conditions and the production environment.
2

Differential regulation of signalling pathways for insulin and insulin-like growth factor I

100%
Lopaczynski W.
Acta Biochimica Polonica
|
1999
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tom 46
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nr 1
The insulin receptor (IR) and the insulin-like growth factor receptor I (IGF-IR) have different functions in cell growth, apoptosis, differentation, and transformation. Although some of these differences may be explained by the relative level of receptor expression and receptor structure (a and b subunits), they may also be attributed to differences in intracellular signals generated by insulin and IGF-I. The presence of hybrid receptors (IR ab subunits and IGF-IR ab subunits) making up the heterotetramers has added a new dimension to our understanding of the functional roles of these receptors. However, to date the results of efforts to understand the differences between these two closely related receptors have indicated mostly similarities. For example, both receptors utilize IRS-1/IRS-2 and Shc as immediate downstream adaptors, leading to activation of the Ras, Raf, ERK kinases and PI-3 kinase pathways. We have used the yeast two hybrid system to identify proteins which bind to the activated IGF-IR but not to the IR. The cytoplasmic domain of the IGF-IR was used to screen a human fetal brain library and two isoforms of the 14-3-3 family were identified. 14-3-3 proteins are a highly conserved family of proteins which have recently been shown to interact with other components of the mitogenic and apoptotic signaling pathways, including Raf, BAD, Bcr/Bcr-Abl, middle-T antigen, Ksr, PKC, PI-3 kinase, ASK1 kinase, and cdc25C phosphatase. We also identified human Grb10, an adaptor protein with SH2 domain associated with the IGF-IR b subunit. Smith's laboratory showed that Grb10 preferentially binds to the IR in intact cells. Using the interaction trap screen (active cytoplasmic domain of the IGF-IR) 55PIK and SOCS-2 proteins were also identified. However, 55PIK and SOCS-2 also interact with the IR in the yeast two hybrid system. These studies raise the possibility that 14-3-3 and Grb10 may play a role in insulin and IGF-I signal transduction and may underlie the observed differences.
3

Evaluation of mouse preimplantation embryos cultured in media enriched with insulin-like growth factors I and II, epidermal growth factor and tumour necrosis factor alpha

86%
Kurzawa R., Glabowski W., Wenda-Rozewicka L.
Folia Histochemica et Cytobiologica
|
2001
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tom 39
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nr 3
4

Insulin-like growth factor I activates insulin receptor substrate 1 and Ras in human osteosarcoma cells

86%
Lopaczynski W., Terry C.
Acta Biochimica Polonica
|
1999
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tom 46
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nr 1
Insulin-like growth factor I (IGF-I) stimulates multiplication of the human osteosarcoma cell line, MG-63, by acting through IGF-I receptor. We have characterized IGF-I stimulated phosphorylation of IRS-1, activation of Ras cycle and phosphorylation of c-Jun in this cell line. Serum starved MG-63 cells were (1) IGF-I stimulated and lysates were immunoprecipitated with polyclonal IRS-1 antibody or (2) metabolically labeled with [32P]orthophosphoric acid and then cells were treated with IGF-I. Cell lysates were immunoprecipitated with p21Ras antibody(Y13-259) and bound nucleotides were analysed by thin layer chromatography. We demonstrated tyrosine phosphorylation of IRS-1/2 immunoprecipitated from MG-63 cells stimulated with IGF-I. We also showed an increased level of GTP in p21Ras immunoprecipitates from IGF-I treated cells. Nuclear extracts prepared from 32P-labeled cells before and after addition of IGF-I were immunoprecipitated with c-Jun antibody. After electrophoresis and autoradiography, phosphorylation of the c-Jun band was seen to be IGF-I independent. Phosphoamino acid analysis of the c-Jun band showed that phosphoserine was the major species.
5
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Content available

Relationship between receptors for insulin-like growth factor - I, steroid hormones and apoptosis-associated proteins in canine mammary tumors

72%
Dolka I., Motyl T., Malicka E., Sapierzynski R., Fabisiak M.
Polish Journal of Veterinary Sciences
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2011
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tom 14
|
nr 2
In the veterinary literature there are few data concerning the expression of insulin-like growth factor type I (IGF-IR) in the canine mammary gland tumors. The aim of the present study was the evaluation of IGF-IR expression and its correlation to the expression of estrogen receptor α (ERα) and progesterone receptor (PR), proteins: Bcl-2, Bax, p53 in canine mammary gland tumors, and also a correlation with other features: bitch’s age, tumor diameter, histologic type of tumor, degree of histologic malignancy, proliferate activity. The study was done on 112 epithelial neoplasms: 21 (19%) were adenoma, 38 (34%) complex carcinoma (adenocarcinoma), 47 (42%) simple carcinoma (adenocarcinoma) and 6 (5%) solid carcinoma. Histochemistry and immunohistochemistry methods were employed. It was shown that more common and/or higher IGF-IR expression in cells of canine mammary gland tumors was related to the histologic type of cancer of worse prognostic (solid and simple carcinoma), high histologic degree of malignancy (IIIo) but the statistical analysis did not reveal any significant differences. We observed the high degree of IGF-IR expression in tumors which displayed the high ERα and PR expression. These results suggest the involvement of IGF-IR in the development of hormonosensitive canine mammary tumors. Additionally, the significant positive correlation between expression of IGF-IR and p53, Bax was found. Our study provides some evidence that interactions exist between the IGF-IR and these apoptosis-associated proteins may contribute to the development and progression of canine mammary gland tumors. These results require further investigations.
6

The mitogenic and collagen biosynthesis stimulating activities in the serum of rats with the methylcholanthrene induced sarcoma

72%
Palka J., Sobolewski K., Bankowski E.
Acta Biochimica Polonica
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1993
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tom 40
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nr 1
7

Differences in the secretion pattern of growth hormone and insulin-like growth factor-I in sheep of two crossbreeds after dietary protein restriction

72%
Wojcik-Gladysz A., Krejci P., Polkowska J.
Journal of Animal and Feed Sciences
|
2002
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tom 11
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nr 4
8

Chemotactic effects of insulin-like growth factors on mouse sperm

72%
Sliwa L.
Acta Biologica Cracoviensia. Series Zoologia
|
2001
|
tom 43
9

Binding of the basic fibroblast growth factor [bFGF] by soluble components of human umbilical cord

72%
Sobolewski K., Bankowski E., Palka J., Jaworski S.
Acta Biochimica Polonica
|
2002
|
tom 49
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nr 4
Pre-eclampsia, the most common pregnancy associated syndrome, is connected with remodelling of extracellular matrix of the umbilical cord tissues. Since the fibroblast growth factor (FGF) is known to be a stimulator of collagen and glycosaminoglycan biosynthesis, one may expect that it plays an important role in such a remodelling. Studies performed on the umbilical cords of 10 control and 10 pre-eclamptic new­borns demonstrated that both the umbilical cord arterial wall and Wharton's jelly con­tain FGF mainly in complexes with the components of different molecular mass. Pre-eclampsia is associated with a decrease of endogenous FGF-binding by soluble high molecular mass components of the umbilical cord. It is suggested that FGF re­leased from these complexes may be actively bound by fibroblasts of the umbilical cord, stimulating them to produce collagen and sulphated glycosaminoglycans.
10

Associations of the IGF2 gene with growth and meat efficiency in Large White pigs

72%
Kolarikova O., Putnova L., Urban T., Adamek J., Knoll A., Dvorak J.
Journal of Applied Genetics
|
2003
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tom 44
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nr 4
11
Content available remote

Changes in insulin like growth factors, myostatin and vascular endothelial growth factor in rat Musculus latissimus dorsi by poly-3-hydroxybutyrate implants

58%
Gredes T., Spassov A., Mai R., Mack H., Loster B. W., Mazurkiewicz-Janik M., Kubein-Meesenburg D., Fanghanel J., Gedrange T.
Journal of Physiology and Pharmacology. Supplement
|
2009
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tom 60
|
nr 3
77-81
12
Content available remote

Membrane estrogen receptors - is it an alternative way of estrogen action?

58%
Soltysik K., Czekaj P.
Journal of Physiology and Pharmacology
|
2013
|
tom 64
|
nr 2
13

IGFBP7 aggravates sepsis-induced acute lung injury by activating the ERK1/2 pathway

58%
Xu Q., Wang J.
Folia Histochemica et Cytobiologica
|
2020
|
tom 58
|
nr 4
14
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Polymorphism of insulin like growth factor IGF-1 in position 211 in national sheep breeds with carped wool compared to Polish Merino and European Muflon (Ovis aries musimon)

58%
Niznikowski R., Czub G., Glowacz K., Swiatek M., Slezak M.
Annals of Warsaw University of Life Sciences - SGGW. Animal Science
|
2013
|
tom 52
Research was carried out on 1,684 sheep (1,093 F and 591 M) originating from European Mouflon (Ovis aries musimon), its hybrids with Polish heat sheep and four sheep breeds that are characterized by a mixed wool compared to Polish Merino. All animals were subjected to gene identification factor insulin-IGF-1, in the assessment of allele C and T. In conclusion it should be noted that in the 7 examined breed groups sheep showed no polymorphism alleles and genotypes of insulin-like factor gene IGF-1, limiting its scope to determine the C allele and genotype CC. This result indicates the need for further research in this area in "culture" sheep imported and adapted to Polish conditions and the production environment.
15

Changes of insulin-mediated protein kinases phosphorylation and the expression of IGFBP-3 in skeletal muscle of streptozotocin-diabetic mice

58%
Grzelkowska-Kowalczyk K., Wieteska W.
Polish Journal of Veterinary Sciences
|
2005
|
tom 08
|
nr 3
16
Content available remote

The effect of endurance training on muscle strength in young, healthy men in relation to hormonal status

58%
Grandys M., Majerczak J., Duda K., Zapart-Bukowska J., Sztefko K., Zoladz J. A.
Journal of Physiology and Pharmacology. Supplement
|
2008
|
tom 59
|
nr 7
17

Osteoblast differentiation of NIH3T3 fibroblasts is associated with changes in the IGF-I-IGFBP expression pattern

58%
Abdallah B. M.
Cellular and Molecular Biology Letters
|
2006
|
tom 11
|
nr 4
Insulin-like growth factors (IGFs) and IGF-binding proteins (IGFBPs) are essential regulators for osteoblast proliferation and differentiation. It has been reported that Dexamethasone (Dex), an active glucocorticoid (GC) analogue, synergizes the stimulatory effect of 1,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)) on osteoblast differentiation in the mouse fibroblastic cell line NIH3T3. I investigated whether this stimulatory effect is associated with changes in the expression pattern of the IGF/IGFBP system. Quantitative real-time PCR technology was used to quantify the gene expression levels of the IGF-system during osteoblast differentiation and in response to 1,25(OH)(2)D(3) or Dex alone under serum-containing and serum-free culture conditions. Interestingly, NIH3T3 was shown to express high mRNA levels of IGF-I, IGF-II and IGFBP-5, and low levels of both IGFBP-2 and-6. During osteoblast differentiation (days 6-12), IGF-I mRNA was repressed by more than 60%, while the transcript of IGFBP-5 was markedly up-regulated, by more than 50-fold. Similarly, treatment with Dex alone resulted in a dose-and time-dependent increase in the expression of IGFBP-5 and a decrease in IGF-I mRNA. Treatment with 1,25(OH)(2)D(3) alone increased the mRNA levels of IGF-I and IGFBP-6 by around 4-and 7-fold, respectively, in a dose-and time-dependent manner. In conclusion, my data demonstrated that osteoblast differentiation of NIH3T3 is associated with changes in the expression pattern of IGFs/IGFBPs, which are regulated by glucocorticoid in the presence of 1,25(OH)(2)D(3). Modulation of the IGF/IGFBP levels by glucocorticoid might suggest important roles for the IGF-system in mediating the osteoblast differentiation of the NIH3T3 cell line.
18

Use of modern genetics achievements for improvement of pork quality - a review

58%
Pierzchala M., Pareek C. S., Kuryl J.
Polish Journal of Food and Nutrition Sciences
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2006
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tom 15/56
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nr 4
According to the FAO database [2002], approximately 40% (94 million metric tons) of the red meat consumed annually worldwide is pork. Pork consumption has been increasing consistently with the increase of world population. In the past decade, modern research achievements towards genetic improvement of economic traits, like growth rate, based on studies of myogenesis and metabolomics of adipose tissue, have had a major impact on improving the carcass composition, meat quality and efficiency of the pork production (in swine industry). These technologies based on research in functional genomics, have a significant potential, but considerable research effort will be required before they can effectively be utilized in pig production. Knowledge about the sequence of the pig genome would help to identify new candidate genes and unique regulatory elements. This great promise provides new information about regulation of expression of such genes that can be used to enhance efficiency of pork production in the future. The aim of this study was to assess a comprehensive overview on functional candidate genes related especially to myogenesis, for examples: growth hormone (GH), growth hormone receptors (GHR), growth hormone realizing hormone (GHRH), growth hormone realizing hormone receptors (GHRHR), insulin like growth factors and their receptors (IGF, IGF-I, IGF-II, IGF-IR), pituitary-specific transcription factor 1 (PIT-1 renamed as POU1F1), leptin (LEP), leptin receptors (LEPR), myogenic regulatory factors gene family (MRF), the protein kinase adenosine monophosphate-activated γ3-subunit (PRKAG3) and the melanocortin receptor gene family (MCR), for body growth rate and carcass composition traits towards their functional role for the genetic improvement of meat quality and efficiency of the pork production.
19

Growth factor/growth factor receptor loops in autocrine growth regulation of human prostate cancer DU145 cells

51%
Ligeza J., Ligeza J., Klein A.
Acta Biochimica Polonica
|
2011
|
tom 58
|
nr 3
Autocrine growth factors produced by epithelial cells mediate the development and proliferation of neoplastic human prostate tissue. Various approaches have been used to down-regulate neoplastic growth of prostate cancer using natural flavonoids, soluble receptors, pseudo-ligands, monoclonal antibodies and tyrosine kinase inhibitors (tyrphostins). Selected growth factor/growth factor receptor loops (mainly TGFα/EGFR and IGFs/IGFIR) have been proposed as regulators of prostate cancer cell growth. We have previously determined that blockade of IGFIR or VEGF2R signaling pathways by tyrphostin AG1024 and SU1498 inhibits autocrine growth and viability of DU145 cells in vitro. Recently, we compared the activity of AG1024 and SU1498 with the inhibiting effect of tyrphostin A23 (a selective inhibitor of EGFR). The results described in this paper confirm that DU145 cells do not produce IGFI or EGF. In contrast, DU145 cells produce a great amount of VEGF, much more than TGFα (about 60-fold), and VEGF may be the real autocrine growth factor of the investigated cells. The results indicate that the growth of DU145 may be regulated by at least three autocrine loops: TGFα/EGFR, IGFII/IGFIR and VEGF/VEGFR2. Neither AG1024 nor SU1498 affected the production of TGFα substantially, which excludes the possibility that IGFRs or VEGFR2 inhibitors arrest the growth of these cells by inhibition of synthesis and/or secretion of TGFα. The obtained data indicate that all tree investigated tyrphostins (AG1024, SU1498 and A23) inhibit signal transmission by Akt (PKB), ERK(1/2), Src and STAT in a similar manner. A comparison of the effects of the investigated tyrphostins indicates that TGFα, IGFII and VEGF stimulate cell growth by affecting the same signaling pathway. The hypothesis was confirmed by the effect of the investigated tyrphostins on activation of EGFR. All these inhibitors decreased phosphorylation of EGFR to the same extent, and after the same time of incubation with cell culture. These results strongly suggest that stimulation of EGFR kinase is the main step in the initiation of mitogen signaling in DU145 cells, regardless of the type of ligand (TGFα, IGFs or VEGF) and their specific receptors.
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