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The population of spider feeding on insect pests of cotton was observed throughout the growth period of the crop, being maximum in the month of July. Chrysoperla was observed from second fortnight of June to harvesting of the crop being maximum in November. The incidence of coccinellids was recorded from the middle of August to middle of September and remained till harvesting of the crop being maximum in the first fortnight of November. A positive significant effect of maximum and minimum temperature on spider and negative significant effect on population of chrysoperla and coccinellids was recorded. Evening RH exerted positive significant effect on spider and negative significant effect on coccinellids.
Entomopathogenic fungi have great potential as biological control agents against insect pests. So they are being developed worldwide for the control of many pests of agricultural importance. But their effect on non-target insects, such as natural enemies has been a matter of great concern. Recently we came across an entomopathogen infecting a laboratory culture of Zygogramma bicolorata, a potential biocontol agent of noxious weed, Parthenium hysterophorus. The pathogen was isolated from the grubs and beetles and identified as Beauveria bassiana. In the present paper, the entomopathogen, B. bassiana is reported and described for the first time from the laboratory culture of Z. bicolorata.
The effects of powdered herbs (lavender Lavandula officinalis L., peppermint Mentha piperita L. and basil Ocimium basilicum L.) added to the food substances of three insect species: the rice weevil Sitophilus oryzae (L.), lesser grain borer Rhyzopertha dominica (F.), and the saw-toothed grain beetle Oryzaephilus surinamensis (L.) on the life span and development of the three insect species as well as habitat conditions, sex structure, and the survivability of males and females were considered. The experiments were conducted in the laboratory at a temperature of 28°C and relative humidity (RH) 60 ±5%. In all the studied populations, high female mortality was found (♂/♀ < 1). The studied populations were: the rice weevil and the lesser grain borer cultured in wheat grain with herb additions, and the saw-toothed grain beetle cultured in semolina with herb additions. Among the herbs used, peppermint caused a significant effect in the form of increased mortality rates among females of S. oryzae and O. surinamensis. Lavender and basil had a similar impact among R. dominica females. The herbs applied in the experiments intensified the mortality of females and, consequently, led to the disappearance of entire populations.
Bioassays were conducted to assess the effects of two silicon dioxide nanoparticles of Aerosil® and Nanosav against adults of Rhyzopertha dominica F. and Tribolium confusum Jacquelin du Val. Silica nanoparticles were applied at the rates of 50, 100, 200 and 300 mg · kg–1 on wheat and peeled barley. The mortality was counted after 1, 2, 3, and 7 days of exposure. Another experiment was carried out to evaluate the effect of food source on the survival of beetles after exposure to silica nanoparticles. Adults were exposed to silica nanoparticles at the rate of 0.2 mg · cm–2 for 1 and 2 days on filter paper inside plastic Petri dishes, respectively. After exposure, the initial mortality was counted and live individuals of both species were held for a week in empty glass vials or vials containing wheat and wheat flour, respectively. Silica nanoparticles have high toxicity on R. dominica and T. confusum adults. Rhyzopertha dominica was more susceptible than T. confusum. However, the mortality of both species increased with increasing concentrations and time exposed to each concentration. At low concentrations, Aerosil® was more effective than Nanosav. Silica nanoparticles were more effective in wheat grains than barley. Results indicated that the initial mortality was so high that the impact of food source on delay mortality was unclear in most cases. Silica nanoparticles were efficient against tested species and can be used effectively in a stored grain integrated pest management program.
The aim of this investigation was to survey the occurrence, biodiversity, and toxicity of Bacillus thuringiensis strains originating from dead caterpillars of the forest pest, Lymantria dispar (Lepidoptera). Morphological, biochemical, and microscopic identification of isolates from the insects showed the presence of five different Bacillus species, including 2% of B. thuringiensis. Based on the biochemical profiles, the B. thuringiensis were determined to be B. thuringiensis finitimus-like and B. thuringiensis alesti-like bacilli. Both produced spherical inclusions composed of three or five protoxins. The molecular weights of these proteins varied from 20 to ca. 64 kDa. Mixtures of spores/inclusions of the B. thuringiensis were tested for their toxicity against larvae of Drosophila melanogaster. The mortality levels of the larvae caused by these spores and crystalline inclusions varied from 5 to 15%. The lethal doses (LD₅₀) of these isolates against D. melanogaster were 8.8 x 10¹² spores/ml for B. thuringiensisfinitimus and 1.3 x 10¹⁸ spores/ml for B. thuringiensis alesti.
Studies on seed storage were conducted at seed processing and storage section of the Institute of Agricultural Research and Training, Obafemi Awolowo University, Moor Plantation, Ibadan Nigeria in 2007 and 2008, to evaluate High protein maize (HPM) seed for resistance/tolerance to storage insect pest, and assess the resultant effect of seed treatment chemicals on its germination potential. Seeds of HPM variety (ART-98-SW1) and Quality protein maize (QPM) variety (ILE-1-OB) and two varieties of field corn (SUWAN-1-SR and TZPB-SRW) were treated with chemicals, namely Fitscophos™, Actellic 25 EC™, Apron Star™ and combination of Actellic 25 EC and Fitscophos before storing them under controlled environment for six months. The results show that HPM, QPM and field corn require storage chemicals for effective storage, but both QPM and HPM varieties were significantly damaged by storage insect pests compared to field corn varieties regardless of the chemical used. Apron Star effectively reduced infestation by insect pests, but it may bring about significant reduction in seed viability if used for long term storage. Combination of Actellic 25 EC and Fitscophos successfully reduced infestation by insect pests without significant injurious effect on seed viability. Breeding programmes for resistance to storage pests is recommended while long term effects of Apron Star on seed viability needs to be investigated before recommending it for use in HPM/QPM maize varieties.
Currently, the use of insecticides is an acute problem. Due to rapid population growth, the primary task is to increase food production. Beyond abiotic factors (drought, soil salinity, etc.) that reduce crop yields, farmers face problems with insect pests that can decrease crop productivity up to 60%. Also, insects are carriers of severe viral and protozoan human diseases. The need for application of insecticides is not questioned but many of them cause resistance of insect pests to them. This, in turn, leads to the necessity to invent new insecticides that are safe and more effective for long-term use. Preparations based on conservative parts of nucleic acids, particularly contact DNA insecticides, could be used to solve insecticide resistance problem as control agents which are well-tailored to target insect pests. This mini review is devoted to these issues.
This study investigated insect pest infestation of maize, millet, sorghum, rice and wheat grains traded in the three main markets namely Monday, Baga-Road and Custom-Park markets in Maiduguri, Borno state, Nigeria. Sampling lasted from January to June 2012. Samples per grain type, each weighing one kilogram, were obtained every two weeks from ten randomly selected traders per market. Each sample was sieved in the laboratory to recover all adult insects present, both at sampling and also after one month stay (laid out in a completely randomized design). In each market, Sitophilus zeamais Motschulsky and Rhyzopertha dominica (F.) followed by Tribolium castaneum Herbst, Sitophilus oryzae (Linnaeus), Trogoderma granarium (Everts) or Cryptolestes sp. were the insect pests commonly found attacking more (between two to five) grain types. Observed highest and lowest mean insect numbers were respectively by S. zeamais in maize grains (12.13 individuals in Monday market) and T. granarium in wheat grains (0.13 individuals in Baga-Road market). Results unveil that among three markets, higher mean total numbers of insects were present in maize, rice or wheat (18-33). For all grain types, the mean numbers of damaged grains were significantly higher in Monday (53-357) and Custom-Park (46-302) than in Baga-Road (17-109) markets. Furthermore, the mean total number of damaged grains across markets was significantly higher maize, followed by rice and wheat (55-231) than in other grain types (37-41). The implications of all these results on insect infestation of different cereal grain types sold in these markets are discussed.
A field study was carried out during the two cropping seasons of okra to investigate the efficacy of plant extracts and synthetic insecticides applied singly and synergistic effect of botanicals with the synthetic insecticide on some insect pests of okra. Plots were arranged in a Randomized Complete Block Design (RCBD), with three replications. Three insect pests of okra (Podagrica uniformis, Podagrica sjostedti and Zenocerous variegatus) were observed on the field. Cypermethrin applied singly and Cypermethrin combined with plant extracts effectively controlled observed insect pests, followed by lone application of plant extracts (L camara, T. vogelii and Z. officinales plant extracts at 1st and 2nd season. Okra yield revealed that both plant extract and Cypermethrin combined with plant extract were not significantly different from one another at first season and at second season Cypermethrin combined with plant extracts performed better than Cypermethrin applied singly. This study indicates that the studied plant extracts can be good synergist for Cypermethrin in controlling field pest of okra.
Extensive studies have been carried out to identify plant proteins with insecticidal properties towards insects. The paper describes a test for screening toxicity and growth inhibition of five commercially available proteins such as: bromelain, honey bee venom, two lectins-wheat germ agglutinin (WGA), concanavalin A (Con A) and lysozyme in artificial diets on Sitobion avenae /F./. Grain aphid proved to be sensitive to all tested proteins. Among the tested proteins concanavalin A and wheat germ agglutinin had the highest negative influence on feeding, reduced weight and increased mortality of tested aphids independently from the dose. It suggested that this group of the proteins have an insecticidal activity and is a good candidate for control of the insect pests.
The paper presents an evaluation of health condition of selected oak stands in the Włoszakowice Forest Division based on a synthetic tree damage index. Field work was conducted in 2007-2008. In order to perform this task seven experimental sites were established, with 25 trees from Kraft’s age classes I-III evaluated in each. It was found that the overall health condition of most stands deteriorated in 2008 in comparison to 2007.
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