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Carbon nanoparticles as transporters of melittin to glioma grade IV U87 cells in in vitro model

100%

Biniecka P., Jaworski S., Bugajska Z., Daniluk K.

Annals of Warsaw University of Life Sciences - SGGW. Animal Science

2017

tom 56[1]

Carbon nanoparticles as transporters of melittin to glioma cells in in vitro model.Substances derived from nature have natural cytotoxic properties, melittin, the main component of bee venom is one of them. It has the ability to destroy any lipid bilayer, therefore to be used in a cancer treatment it needs to be targeted. The aim is to create the drug delivery system, which would efficiently deliver the active substance to glioma cells. Carbon nanoparticles are considered to be a good agent in biomedical applications, due to their biocompatibility and small sizes. In this study five types of nanoparticles were used: pristine graphene (GN), nanographene oxide (nGO), graphite (G), nanodiamond (UDD) and hierarchical nanoporous carbons (HNCs) to target the melittin to cancer cells. The visualization of the drug delivery complexes of melittin and nanoparticles was done with transmission electron microscopy, the influence of the complexes on cell morphology and structure was pictured with scanning electron microscope. Moreover, in order to check the viability of the cells treated with melittin and the complexes of melittin and nanoparticles the PrestoBlueTM assay was done, also to specify the way of the cell death the annexin V/PI assay was carried out. The results indicate that various nanoparticles behave differently in a complex with melittin. The UDD, GN and nGO nanoparticles resulted in higher mortality than the melittin itself. Creating and applying such complexes of melittin with nanoparticles in glioma cancer treatment may be a promising solution in the therapy.

Investigation on adsorption of fatty and bile acids in the presence of dietary supplements containing chromium

100%

Meler J., Grimling B., Pluta J.

Journal of Elementology

2010

tom 15

nr 1

141-147

Dietary supplements contain not only macro- and microelements, but also elements which affect human metabolism. Many products available on the market contain chromium compounds together with chitosan used as a dietary supplement enhancing the digestion of lipids. The studies involved natural chitosan from krill available on the market, with the deacetylation degree of 85 to 95%, and dietary supplements containing chitosan (Vitana®, Hitec Nutrition®) as well as a product containing ionic chromium with niacin and several aminoacids — Chromdiet®). The study has determined the capability of binding fatty and bile acids by dietary supplements containing chitosan and chromium. The process of lipids and bile acids adsorption was investigated by means of a dynamic method in a biopharmaceutical model imitating in vitro conditions. The findings prove that extracts of fatty acids and bile acids undergo adsorption by various kinds of adjuvant substances found in dietary supplements, which confirms a significant effect of these polymers on the bioavailability of fatty and bile acids in a human organism. The addition of chromium to a supplement does not effect the capability of chitosan to bind fatty and bile acids. Mean adsorption of bile acids by 1 g of the polymer (chitosan, inulin, fibre) ranges from 0.9 g to 1.79 g depending on the pH (which decreases the bioavailability of lipids by 15-30%).

In vitro and in vivo activities of flavonoids - apigenin, baicalin, chrysin, scutellarin - in regulation of hypertension - a review for their possible effects in pregnancy-induced hypertension

80%

Ozarowski M., Kujawski R., Mikolajczak P. L., Wielgus K., Klejewski A., Wolski H., Seremak-Mrozikiewicz A.

Herba Polonica

2019

tom 65

nr 1

Flavonoids and their conjugates are the most important group of natural chemical compounds in drug discovery and development. The search for pharmacological activity and new mechanisms of activity of these chemical compounds, which may inhibit mediators of inflammation and influence the structure and function of endothelial cells, can be an interesting pharmacological strategy for the prevention and adjunctive treatments of hypertension, especially induced by pregnancy. Because cardiovascular diseases have multifactorial pathogenesis these natural chemical compounds with wide spectrum of biological activities are the most interesting source of new drugs. Extracts from one of the most popular plant used in Traditional Chinese Medicine, Scutellaria baicalensis Georgi could be a very interesting source of flavonoids because of its exact content in quercetin, apigenin, chrysin and scutellarin as well as in baicalin. These flavonoids exert vasoprotective properties and many activities such as: anti-oxidative via several pathways, anti-inflammatory, anti-ischaemic, cardioprotective and anti-hypertensive. However, there is lack of summaries of results of studies in context of potential and future application of flavonoids with determined composition and activity. Our review aims to provide a literature survey of in vitro, in vivo and ex vivo pharmacological studies of selected flavonoids (apigenin, chrysin and scutellarin, baicalin) in various models of hypertension carried out in 2008–2018.

Ograniczanie wyników

1 Annals of Warsaw University of Life Sciences - SGGW. Animal Science

1 Herba Polonica

1 Journal of Elementology

1 Biniecka P.

1 Bugajska Z.

1 Daniluk K.

1 Grimling B.

1 Jaworski S.

1 Klejewski A.

1 Kujawski R.

1 Meler J.

1 Mikolajczak P. L.

1 Ozarowski M.

1 Pluta J.

1 Seremak-Mrozikiewicz A.

1 Wielgus K.

1 Wolski H.

1 2019

1 2017

1 2010

Wyniki wyszukiwania

Carbon nanoparticles as transporters of melittin to glioma grade IV U87 cells in in vitro model

Investigation on adsorption of fatty and bile acids in the presence of dietary supplements containing chromium

In vitro and in vivo activities of flavonoids - apigenin, baicalin, chrysin, scutellarin - in regulation of hypertension - a review for their possible effects in pregnancy-induced hypertension