Wyniki wyszukiwania

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In vitro evaluation of antagonistic microorganisms for the control of die-back of neem causal agent Phomopsis azadirachtae

100%

Girish K., Bhat S. S., Raveesha K. A.

Journal of Plant Protection Research

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2009

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tom 49

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nr 4

362-368

The die-back of neem caused by Phomopsis azadirachtae is a devastating disease in India reducing the life span and seed production of neem. Six isolates of antagonistic bacteria and fungi, Bacillus cereus (MTCC 430), B. subtilis (MTCC 619), Pseudomonas aeruginosa (MTCC 2581), P. oleovorans (MTCC 617), Trichoderma harzianum (MTCC 792) and T. viride (MTCC 800) were evaluated against P. azadirachtae under in vitro conditions. Culture filtrates of all these microorganisms were extracted using ethyl acetate, and the obtained fractions were tested for their antifungal activity against P. azadirachtae at different concentrations. Ethyl acetate extracts of B. subtilis and P. aeruginosa were highly effective and completely inhibited the growth of P. azadirachtae at 25 ppm concentration. Both these isolates may be considered as factors for the biological control of die-back of neem.

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In vitro and in vivo evaluation of antitrypanosomal activity of Annona muricata stem bark extracts

100%

Onyeyili P. A., Aliyoo K.

Herba Polonica

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2015

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tom 61

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nr 2

The control of trypanosomosis in animals and humans based on chemotherapy is limited and not ideal, since the agents used are associated with severe side effects, and emergence of relapse and drug resistant parasites. The need for the development of new, cheap and safe compounds stimulated this study. Three concentrations (211, 21.1 and 2.11 mg per ml) of chloroform stem bark extract of Annona muricata were screened for trypanocidal activity against Trypanosoma brucei brucei in vitro. Also, two doses (200 mg per kg and 100 mg per kg) of the extract were evaluated for trypanocidal activity in rats infected with the parasite. Haematological parameters were determined on day 1 post infection and on days 1, 6 and 30-post extract treatment. The extracts inhibited parasite motility and totally eliminated the organisms at the concentrations used in vitro. The extract also showed promising in vivo trypanocidal activity. The observed in vitro and in vivo trypanocidal activities may be due to the presence of bioactive compounds present in the extracts as seen in this study. The extract also improved the observed decreases in haematological parameters of the treated rats, which may be due to their ability to decrease parasite load. The observed oral LD50 of 1,725.05 mg per kg of the chloroform A. muricata extract using up and down method is an indication of very low toxicity, implying that the extract could be administered with some degree of safety.

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A nanoformulation of siRNA and its role in cancer therapy: In vitro and in vivo evaluation

100%

Jagani H., Rao J. V., Palanimuthu V. R., Hariharapura R. C., Gang S.

Cellular and Molecular Biology Letters

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2013

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tom 18

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nr 1

Overexpression of anti-apoptotic Bcl-2 is often observed in a wide variety of human cancers. It prevents the induction of apoptosis in neoplastic cells and contributes to resistance to chemotherapy. RNA interference has emerged as an efficient and selective technique for gene silencing. The potential to use small interfering RNA (siRNA) as a therapeutic agent for the treatment of cancer has elicited a great deal of interest. However, insufficient cellular uptake and poor stability have limited its therapeutic applications. The purpose of this study was to prepare chitosan nanoparticles via ionic gelation of chitosan by tripolyphosphate for effective delivery of siRNA to silence the anti-apoptotic Bcl-2 gene in neoplastic cells. Chitosan nanoparticles loaded with siRNA were in the size range 190 to 340 nm with a polydispersive index ranging from 0.04 to 0.2. They were able to completely bind with siRNA, provide protection against nuclease degradation, and enhance the transfection. Cell culture studies revealed that nanoparticles with entrapped siRNA could efficiently silence the antiapoptotic Bcl-2 gene. Studies on Swiss albino mice showed that siRNA could be effectively delivered through nanoparticles. There was significant decrease in the tumor volume. Blocking the expression of anti-apoptotic Bcl-2 can enhance the sensitivity of cancerous cells to anti-cancer drugs and the apoptosis rate. Therefore, nanoformulations with siRNA can be promoted as an adjuvant therapy in combination with anti-cancer drugs.

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In vitro evaluation of electroporated gold nanoparticles and extremely-low frequency electromagnetic field anticancer activity against Hep-2 laryngeal cancer cells

100%

Alshehri M. A., Wierzbicki P. M., Kaboo H. F., Nasr M. S. M., Amer M. E., Abuamara T. M. M., Badr D. A., Saleh K. A., Fazary A. E., Mohamed A. F.

Folia Histochemica et Cytobiologica

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2019

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tom 57

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nr 4

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Phytochemical characterization by GC-MS and in vitro evaluation of antiproliferative and antimigratory studies of Leucas aspera leaf extracts on MDA-MB-231 cell line

100%

Mahaboob Begum S.M. F., Sankarram M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2024

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tom 105

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nr 1

6

In vitro evolution and characterization of a copper resistant strains and populations of Chlamydomonas reinhardtii

80%

Plucinski B., Waloszek A., Strzalka K.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

Ograniczanie wyników

In vitro evaluation of antagonistic microorganisms for the control of die-back of neem causal agent Phomopsis azadirachtae

In vitro and in vivo evaluation of antitrypanosomal activity of Annona muricata stem bark extracts

A nanoformulation of siRNA and its role in cancer therapy: In vitro and in vivo evaluation

In vitro evaluation of electroporated gold nanoparticles and extremely-low frequency electromagnetic field anticancer activity against Hep-2 laryngeal cancer cells

Phytochemical characterization by GC-MS and in vitro evaluation of antiproliferative and antimigratory studies of Leucas aspera leaf extracts on MDA-MB-231 cell line

In vitro evolution and characterization of a copper resistant strains and populations of Chlamydomonas reinhardtii