Znaleziono wyników: 3

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: immunomagnetic separation

Sortuj według:

Ogranicz wyniki do:

Immunomagnetic detection of cancer cells in pleural effusion of generalized cancer

100%

Kacprzak G., Kolostova K., Kolodziej J., Pawlak I., Rzechonek A., Bobek V.

Folia Histochemica et Cytobiologica

2013

tom 51

nr 3

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Rapid and specific detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation combined with loop-mediated isothermal amplification

100%

Qin Y., Puthiyakunnon S., Zhang Y., Wu X., Boddu S., Luo B., Fan H.

Polish Journal of Food and Nutrition Sciences

2018

tom 68

nr 2

Escherichia coli O157:H7 is well known for many foodborne outbreaks that lead to fatal infections in human being worldwide. The objective of this study was to develop a rapid and sensitive method for detection of EHEC O157:H7 from ground beef using a method that combined immunomagnetic separation (IMS) with loop-mediated isothermal amplifi cation (LAMP). The EHEC O157:H7 cells were separated with Dynabeads coated with antiEHEC O157:H7 after a short enrichment for 4 h. Then, EHEC O157:H7 was identifi ed by LAMP assay for amplifying and detecting the rfbE gene, which is highly conserved in all EHEC O157:H7 strains and exhibits strain-specific gene expression. The LAMP method results analyzed with real time turbidity measurements showed a high specificity and sensitivity, with a positive detection rate of amplifi cation of EHEC O157:H7 DNA diluted to a minimum equivalent concentration of 1.8 × 101 CFU/mL, which was 10 times more sensitive than the conventional PCR assay. The IMS followed with LAMP could capture and detect a bacterial concentration as low as 3×101 CFU/mL from the meat samples, which was close to the sensitivity of LAMP assay with pure culture. IMS combined with realistic LAMP method is a simple, rapid, highly specific gene amplifi cation technology that is suitable for implementing as a screening assay in basic laboratory and field test for detecting food contamination.

Determination of E. coli 0157 in raw and cooked Doner kebabs by using IMS technique

80%

Arun O. O., Aydin A., Vural A., Ciftcioglu G., Aksu H.

Medycyna Weterynaryjna

2007

tom 63

nr 10

1181-1183

Escherichia coli O157 is one of the major threats to public health due to the consumption of meat and meat products. The microorganism is known as a food-borne pathogen even in the presence of low levels. Therefore, a more sensitive method is required to be used for the detection of the microorganism. Immunomagnetic separation (IMS) is a technique used to improve the sensitivity of the detection. IMS has been used to determine the presence of E. coli O157 in raw and cooked Doner samples in this study. 3 out of 30 raw Doner samples have been found to be contaminated with E. coli O157. None of the cooked samples were carrying the agent.

Ograniczanie wyników

1 Folia Histochemica et Cytobiologica

1 Medycyna Weterynaryjna

1 Polish Journal of Food and Nutrition Sciences

1 Aksu H.

1 Arun O. O.

1 Aydin A.

1 Bobek V.

1 Boddu S.

1 Ciftcioglu G.

1 Fan H.

1 Kacprzak G.

1 Kolodziej J.

1 Kolostova K.

1 Luo B.

1 Pawlak I.

1 Puthiyakunnon S.

1 Qin Y.

1 Rzechonek A.

1 Vural A.

1 Wu X.

1 Zhang Y.

1 2018

1 2013

1 2007

Wyniki wyszukiwania

Immunomagnetic detection of cancer cells in pleural effusion of generalized cancer

Rapid and specific detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation combined with loop-mediated isothermal amplification

Determination of E. coli 0157 in raw and cooked Doner kebabs by using IMS technique