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W prezentowanej pracy określono skład podklas IgG w preparatach ludzkich immunoglobulin do stosowania dożylnego w tym w preparacie wyprodukowanym przez Biomed-Warszawa. Stwierdzono różnice w składzie podklas IgG1 i IgG2 między Bioglobuliną a pozostałymi preparatami. Różnice w składzie podklas nie wpływały na aktywność badanych przeciwciał bakteryjnych i wirusowych.
Role of E. granulosus-specific IgG antibody avidity in evaluation of a natural history of the parasite cysts in the liver, duration of infection and immunogenic cysts activity was studied. Thirty-one patients with liver cysts suspected of echinococcosis were examined; E. Granulosus infection was finally confirmed by a fine needle aspiration biopsy or surgery in 29 cases (93.5%) and two non-parasitic simple cysts in the liver were diagnosed. E. granulosus-specific IgG antibody was found in 21 patients (67.7%). High IgG avidity values were observed in 14 patients with liver cysts in a degcneration stage confirmed by imaging techniques. Low IgG avidity values were mostly detected in children with hypoechogenic echinococcal cysts being in a recent stage of infection. Determination of anti-E. Granulosus immunoglobulin G antibody avidity is a very usefull diagnostic tool for a determination of parasitic cysts activity and their developmental age.
The aim of the studies was to evaluate the usefulness of determining the level of haptoglobin, IgG and lysozyme in sera for monitoring the state of health of breeding polar foxes of different behavior at the preparturient period. It was found that in determining the physiological parameters of unspecific humoral immunity, the behavior of the animals should be borne in mind. Significant differences exist in the examined parameters of unspecific immune responses and behavior of foxes. The highest and statistically significant level of haptoglobin, IgG and lysozyme was noted in fearful foxes in comparison to aggressive and confident animals. It can be assumed that lower phagocytic and bactericidal activity noted in the fearful foxes in comparison to that in the aggressive and confident animals is compensated by a higher bacteriolytic activity of lysozyme and a higher level of serum IgG.
The aim of the study was to investigate the level of IgM and IgG class antibodies, specific to selected pathogens in foals at particular stages during their nursing period. The tests were conducted in a thoroughbred stable. Twelve sera from mares and 12 sera from foals were examined and total protein and gamma globulin fraction levels were estimated as well as the relative level of specific antibodies in IgM and IgG class using the ELISA test and expressed as the value of optical density (OD) against the following strains of bacteria antigens: Staphylococcus sciuri (1), Staphylococcus sciuri (2), Staphylococcus sciuri (3), Staphylococcus xylosus (4), Staphylococcus xylosus (5), Staphylococcus aureus (6), Staphylococcus aureus (7), Streptococcus equi subsp. Zooepidemicus (8), Streptococcus equi subsp. Zooepidemicus (9), Enterobacter aglomerans (10), Rhodococcus equi (11), Salmonella enterica ser Typhimurium (12). Traces of specific Ig R. equi were indicated in the foals sera before suckling whereas the OD value increased to 0.35 in sera following the absorption of colostrum. The profile of specific humoral response against environmental pathogens in nursing foals is dependent not only on antigenic moiety of the pathogen but also on the immunological status of the individual.
Sera of 51 cows, including 8 cows with a nodular form of epizootic bovine leukemia, from cowsheds of a high percent of BLV infection (more than 80%) and their foetuses have been examined. The presence and content of IgG, IgM and IgA in sera of cows and their foetuses was determined by the method of a radial single immunodiffusion using standard kits (Miles). The highest content of IgG (1946 mg/100 ml) was noted in sera of cows with a subclinic form of leukemia and the lowest one (1579 mg/100 ml) in seropositive cows. However, these differences were not statistically significant. The level of serum IgM was significantly higher in healthy cows (536 mg/100 ml) and in sera of seropositive cows (486 mg/100 ml) comparing to cows with a permanent lymphocytosis (419 mg/100 ml) and with histopathological lesions (446 mg/100 ml). The content of IgA ranged in all groups from 57 to 60 mg/100 ml. In a group of foetuses from cows with a permanent lymphocytosis the level of serum IgM was higher (13.9 mg/100 ml) comparing to other groups (16.5—17.6 mg/100 ml). A slightly higher level of all immunoglobulin classes was observed in seropositive foetuses against EBL.
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