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The aim of the study was to compare the effectiveness of calves infected with bovine leukaemia virus with that gained after the administration of FLK-BLV cells and lymphocytes of a leukaemic cow. The experiment was carried out on two groups of animals aged from 6 to 8 months. Each group of the cows was infected intravenously with the same dose of 1.2 x 105 FLK cells or lymphocytes. The third group involving two animals served as the control one. The sera of calves were tested at 2, 4, 6 weeks and 2, 3, 4 and 5 months after infection. The geometric mean titer of FLK infected calves was 3.9; 50.8; 101.6; 80.0; 64.0; 63.5 and 51.2 and in the group infected with lymphocytes - 0.0.; 511.6; 776.0; 2051.0; 1559.0; and 504.1, respectively. Serological examinations by means of the ELISA test revealed specific antibodies at two weeks after infection in the FLK group and at four weeks in the lymphocyte treated group. The highest titers were observed at six weeks in the sera of calves of the first group and at two months in case of the second group. It was found that the highest titer of antibodeis in the calves infected with lymphocytes was 20-fold higher than that of FLK cells infected animals.
By the use of isolated strains of B. nodosus classified as A-F serotypes some trials were undertaken to develop a vaccine against ovine footrot. On the basis of immunoge- nicity three strains of A, C and F serotypes were selected which induced in immunized animals the highest level agglutinins. The strains were used to prepare a condensed vaccine which was applied under field conditions. Under study were two groups of sheep, including 410 animals in the first one and 180 in the second flock inflicted with footrot permanently in the past. In comparison to former years, the vaccine reduced the percentage of infection during the 8 months of observations from 25 to 3.5% in the first flock and from 45 to 3.3% in the second one. The results, though carried out on a limited number of animals, turned out very encouraging. Nevertheless, further examinations are necessary to improve the vaccine by other immunogenic serotypes.
Celem badań przedstawionych w obecnej pracy była charakterystyka cech temperaturowrażliwego mutanta wirusa herpes simplex typu 1 (HSV-1). Wykazano statystycznie znamienne obniżenie wirulencji uzyskanego mutanta ts dla wsobnych linii myszy CFW/Pzh oraz BALB/cPzh oraz jego aktywność immunogenną, określaną możliwością ochrony myszy przed letalnym zakażeniem wirulentnym szczepem HSV-1, w tym również u myszy, które w czasie immunizacji otrzymały Depo-Medrol, preparat o działaniu immunosupresyjnym.
Immunogenic properties of a two-component autovaccine against enterotoxemia and braxy of sheep based on C. perfringens D and C. septicum toxoids have been evaluated. Experiments on 49 pregnant sheep immunized 3-2 weeks before parturition and on 50 lambs at the age of 2-3 months vaccinated twice, showed that the prepared autovaccine induced antibodies against e-toxin and a-toxin at a titre of 4-64 antitoxic units/ml in sheep and 8-64 antitoxic units/ml in lambs. An induced level of antitoxic antibodies protected lambs in a fattening period against enterotoxemia and braxy and sheep against C. septicum toxoinfection.
The aim of the study was to isolate and identify the pathogen responsible for mortality in pigeons, and to choose the most immunogenic strain of Salmonella to prepare a vaccine. The study was conducted in two stages. The first stage consisted of isolating and identifying the strains, as well as preparing vaccine prototypes. The material for bacteriological examination comprised samples of parenchymal organs (heart, liver, spleen), intestines, and swabs from synovial cavities. Three strains were isolated and identified as Salmonella Typhimurium var. Copenhagen by biochemical and serological tests. The isolates were used to prepare three prototype vaccines, which were combined with an adjuvant consisting of Carbomer and Ginseng extract. In the second stage, the immunogenicity of the prototype vaccines was evaluated in 24 racing pigeons aged 6-8 weeks, which were divided into 4 experimental groups. Agglutination antibody titers in the serum of immunized birds were evaluated by the tube agglutination test at days 0, 7, 14, and 28 after vaccination. One of the three experimental vaccines generated higher agglutination titers than the others, and was selected for further testing.
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