Serum, bile and faecal protein extracts from slaughtered sheep harbouring lancet flukes, were used to investigate the range of Dicrocoelium dendriticum proteins provoking systemic and local antibody responses in naturally infected animals. ELISA and Western blot tests showed that the lancet fluke infection induced in sheep, serum and local antibody responses against Tris-buffered saline (TBS) soluble somatic proteins, surface proteins and glycoproteins as well as excretions and secretions (ES) of adult D. dendriticum. Sera of infected sheep contained antibodies against 10-14 somatic polypeptides (molecular mass range: 223-12.5 kDa), against 4 surface proteins and up to 9 surface glycoproteins. Antibodies against lancet fluke antigens were also found in 70% of bile samples and 41% of faecal samples. Approximately 40% out of serum samples analysed, contained antibodies against 10 somatic polypeptides and some 24% samples reacted with 12-14 antigens. Serum, bile and faecal samples from non-infected sheep did not recognise any of the lancet fluke proteins.
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