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The purpose of the study was to evaluate an optimum dose of Aeromonas hydrophila and Aeromonas sobria antigens for the vacccination of carp (Cyprinus carpio L.) and to compare the effectiveness of the vaccination with the antigens inactivated with formalin or thermally and given intraperitoneally (ip) or by immersion (imm). The dose was evaluated with the use of formalin antigens. Doses ranging from 3 x 10⁴ to 6 x 10⁸ cells were given by injection and doses of 3 x 10⁵ to 6 x 10⁶ cells/mL of water were administered in bath. Experiments were conducted at 12°C, 16°C, and 23°C ± 1°C . To compare the effectiveness of the antigens inactivated with formalin and thermally the two doses 3 x 10⁸ cells (ip) and 5- 6 x 10⁷cells/mL (imm) were used. The effect of immunisation was evaluated with challenge tests. The relative percentage of health (RPH) and relative percentage of survival (RPS) of fish were calculated. Doses of 3 x 10⁸ cells, and 5-6 x 10⁷ cells/mL given ip and imm, respectively, were considered the most effective irrespective of the water temperature. No marked differences were found between the administration of the antigen by injection and immersion. Fish manifested a significantly higher level of immunity after the administration of formalin antigen in comparison to that following the administration of the antigen inactivated thermally.
The objective of this study was to evaluate effectiveness of Ovalbumin-LHRH-7 (OL) protein when injected in crude, purified, free or encapsulated forms and using a single vaccination protocol along with CpG, inulin and saponin adjuvants. Fifty six C57BL/6 mice in seven groups (n=8) received various treatments and doses: Group 1 was control; Group 2 and 3 were injected twice with purified or crude OL protein, respectively, 4 wks apart. Group 4 and 5 were injected only once with purified or crude OL protein, respectively. Group 6 was injected only once with a mix of purified OL protein and encapsulated purified OL protein. Group 7 was injected only once with a mix of crude OL protein and encapsulated crude OL protein. There was an immunization effect observed on the I125 LHRH % binding (P<0.05). Antibodies (Abs) against LHRH were identified on week 5 of immunization in groups 2, 3 and 4. Boosting at week 5 caused a significant increase in LHRH antibody (Ab) concentrations in groups 2 and 3. Numbers of pregnant animals and prengnancy rates were suppressed in all treatment groups at various degrees (P<0.05). Numbers of pups born were affected by immunization (P<0.05).Concluding, immunization with OL protein generated either biological or both immunological and biological effects in the most of treatment groups. The study confirmed the earlier findings that purified OL protein with CpG adjuvant is effective in inducing immune response and suppressing reproductive functions. However, the original idea that the non-capsulated antigen/adjuvant mix would work as primary injection, while encapsulated counterpart would mimic booster injections in a single vaccination protocol could not be confirmed in this study. Further studies to determine affecting factors for single-injection LHRH immunization are needed.
The aim of the study was the evaluation of immunological response of piglets born from sows immunised with ADV live vaccine and defining the optimal time for their active immunisation. Two weeks after the final vaccination, there were no differences in the level of serum gB antibodies among the sows and all the sows developed humoural immunity. Maternally derived antibodies (MDA) in the sera of all piglets were above the level considered to be positive until about 11 weeks of their life. Following exposure to the live ADV, a greater number of lymphocytes (including CD2⁺ and CD2⁻ cells) from vaccinated animals expressed the IL-2 receptor (CD25), than those from unvaccinated ones. After virus stimulation, there were also higher expression of CD25 on CD8⁺ T cells in all vaccinated animals, while in non-vaccinated pigs; a decrease in such expression was evident. Based on the obtained results it might be stated that the piglet vaccination against AD at 10 and 14 weeks of life was considered to be optimal. In this age, the animals were still protected by passive immunity, but simultaneously were able to develop an active humoural response. It could be also concluded that the high level of MDA may successfully blocked the developmental of active immunity.
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