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The identification system of horses used in Poland is based on the old classification of coat colours which does not include all colours based on colour inheritance patterns. As a result, some colours are incorrectly described. In order to evaluate the scale of the problem, the accuracy of colour identification was assessed by verifying 5779 pedigrees of horses of three different breeds. The verification was performed based on the colour inheritance patterns using a classification of discrepancies. Such discrepancies were detected in 26 cases and related mainly to diluted colours and black. The problem, however, should not be neglected. The number of incorrectly described horses is probably higher, since not all discrepancies can be detected based only on breeding records. The study demonstrated that some errors could be avoided by updating the classification of coat colours. However, a complete elimination of errors is impossible without genetic testing.
Child-friendly educational procedures consisting in the supporting a child in the mastering of the reading are implemented in two ways: a) an intermediate way (by “developing the readiness), and b) in an immediate way - by learning to read. Both types of actions should merge. Amongst factors determining success in the learning to read and write, P. Bryant and U. Goswami - authors of the casual theory of reading, indicate early-child-hood phonological skills, including: identification and formation of rhymes and alliterations (i.e. the capability to differentiate larger elements than a single sound, but smaller than syllables (rhymes and alliterations)). Child’s susceptibility to, among other things, alliterations results in advances in reading and writing in the subsequent education. A significant part of the reported study was devoted to models of reading education applied in the case of the six-year-olds examined. Ideas on how to “introduce children into the world of writing” are basically realized acc. to two models - a standard and a holistic one (see: Górniewicz, 1989; Arciszewska, 2002; Marzano, Paynter, 2004). In both models, children are provided conditions for developing their “readiness for reading” and “mastering the skill of reading”. The models differ in terms of organization and applied methods of the learning to read (Czerwińska, Narożnik, 1997; Doman, Doman, 1992; Majchrzak, 1995, 2007; Rocławski, 1998; Arciszewska, 2002). Both models involve the acquisition of prosodic elements of language (with various intensity). The presented fragment of a research was aimed at providing an answer to a question on the level of capabilities to identify and form alliterations displayed by the surveyed six-year-old children. In analyses, consideration was given to the number of years the children have been attending to a nursery school and the model of reading education realized therein.
Food-borne infections are among the prominent health hazards. Antibacterial agents (ABA) are usually administered to poultry in Lebanon as antibiotic growth promoters (AGP), which might lead to the dissemination of resistant bacterial strains. e aims of this study were to isolate potential food borne pathogens from poultry and investigate an association between AGP usage and antibacterial resistance (ABR). Isolates were obtained from the culture of cloacae swabs and identified. Escherichia coli was the predominant isolate. There was a significant association between the use of tetracycline and gentamicin as AGP and the number of E. coli isolates resistant to these ABA.
The research aimed to identify two sets of charcoal found in a Hellenistic house in Risan (Montenegro) on the Kotor Gulf. The finds date from the 3rd century BC.The study included macroscopic and microscopic observations. Based on macroscopic observation and taking into account the habitat requirements and the range of particular species near Risan and the Kotor Gulf, the first set of charcoal found near a ceramic vessel was attributed to fir wood (Abies sp.), while the charcoal found in the room “with the treasure” was attributed to oak wood (Quercus sp.). The study has added to the body of evidence in favor of the effective botanical identification of charcoal from archeological finds.
To test whether the internal transcribed spacer 2 (ITS2) region is an effective marker for using in authenticating of the Schisandra chinensis at the species and population levels, separately. And the results showed that the wild populations had higher percentage of individuals that had substitution of C→A at site 86-bp than the cultivated populations. At sites 10-bp, 37-bp, 42-bp and 235-bp, these bases of the Schisandra sphenanthera samples differed from that of S. chinensis. Two species showed higher levels of inter-specific divergence than intra-specific divergence within ITS2 sequences. However, 24 populations did not demonstrate much difference as inter-specific and intra-specific divergences were concerned. Both S. chinensis and S. sphenanthera showed monophyly at species level, yet the samples of different populations shown polyphyly at population level. ITS2 performed well when using BLAST1 method. ITS2 obtained 100% identification success rates at the species level for S. chinensis, with no ambiguous identification at the genus level for ITS2 alone. The ITS2 region could be used to identify S. chinensis and S. sphenanthera in the “Chinese Pharmacopoeia”. And it could also correctly distinguish 100% of species and 100% of genera from the 193 sequences of S. chinensis. Hence, the ITS2 is a powerful and efficient tool for species identification of S. chinensis.
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The binomial computerization method is comparison of certain similarities and differences of plants identification system. The ultimate purpose of the plants identification process is method of using digital tools of database and creating new version of plants identification for constructing the digital keys. The present study of plant identification system is based on computerization processes. The plants species characters are constructing basis on taxonomical literature and followed by suitable classification system and binomial rules.
The capacities of differentiation of Mycobacterium bovis BCG from other members of M. tuberculosis complex species using PCR-RFLP, multiplex PCR, and PCR-based genomic deletion analysis approaches were compared. In the study, mycobacteria isolated from patients suspected of adverse events following vaccination with BCG, primarily classified according presence of RD1 marker as virulent and avirulent mycobacteria, were used. The PCR-based genomic deletion analysis was found the best option for mycobacteria diagnostics improvement, as it was capable precisely differentiate virulent and avirulent mycobacteria or virulent species of M. tuberculosis complex. The routine confirmation of mycobacteria species in the cases of adverse events following BCG vaccination is highly expected, especially in clinical practice of patients with primary immunodeficiency.
Studies were conducted on the improvement of A. culicicola identification. This species is phenotypically very similar to A. veronii biotype sobria, A. sobria, and A. allosaccharophila. The sequences of 16S rDNA of A. culicicola isolates show the highest similarity with A. jandaei, A. veronii, and A. caviae. Digestion of 16S rDNA PCR product with Alul and Mbol restriction endonucleases allowed discriminating A. culicicola from all other Aeromonas species with the exception of A. jandaei. Additional digestion of 16S rDNA PCR product with BceAI showed a possibility of distinguishing A. jandaei from A. culicicola.
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