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The activity of hydrolases of larval stages of Anisakis simplex [Nematoda]

100%

Lopienska-Biernat E., Zoltowska K., Rokicki J.

Wiadomości Parazytologiczne

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2004

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tom 50

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nr 3

2

Geotrichum candidum Link 1809: hydrolases activity and own methoof digestive tract strains biotyping

100%

Kurnatowska A., Bialasiewicz D.

Wiadomości Parazytologiczne

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2001

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tom 47

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nr 4

Hydrolase activity of (enzymograms, biotypes) in Geotrichum candidum, one of the poorly described pathogenic fungi, was studied 81 strains were isolated from oral cavity and faeces of patients with gastrointestinal tract disorders. Axenic strains were differentiated with API 20C Aux and API ZYM tests. Then, enzymograms and biotypes were determined for all strains based on the activity of 19 hydrolases. High variability of enzymograms (17 different types) was found. The highest activity was noted in case of: e2-alkaline phosphatase, e6-lecucine arylamidase, e11-acid phosphatase. E5-lipase. e7-valine arylamidase, e12 - naphtol-AS-BI-phosphohydrolase and e17-β-glucosidase were used for biotyping procedures. Our own system of biotyping of 81 strains of G. candidum was based on the mathematical binominal distribution formula (1 : 4 : 6 : 4: 1) – all; ,,+”;one „-”, three „+”; two „-”,two „+”; three ,,-”,one ,,+”; all „-”. We have found: A (11.1 ± 3.5%), B1 (6.17 ± 2.67%). B2 (1.23 ± 1.22%), B4 (4.94 ± 2.41%), C1 (1.23 ±1.22%), C3 (63.0 ± 5.4%), D2 (9.88 ±3.31%), D3 (2.47 ± 1.72%). Among all strains from 8 various biotypes of G. candidum.

3

Effect of avocado oil on the induction of yeast hydrolases

86%

Malajowicz J., Rochalska A., Waclawek W.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2020

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tom 601

4

The activity of hydrolases of entomopathogenic nematodes

86%

Zoltowska K., Lopienska E.

Wiadomości Parazytologiczne

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2003

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tom 49

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nr 4

Similar patterns of hydrolases were observed in three species representing two genera of entomopathogenic nematodes: Steinernema affinis, S. feltiae, and Heterorhabditis zealandica. The same enzymes were present in the studied nematodes but they differed in the level of activity of individual sub-classes of enzymes. A higher activity of esterases and proteolytic enzymes could be observed for H. zealandica than for S. affinis and S. feltiae. On the other hand, the activity of glycosidases in steinernematids was generally much higher than in H. Zealandica.

5

Activity of selected hydrolases in excretion-secretion products and extracts od adult Contracaecum rudolphii

86%

Dziekonska-Rynko J., Rokicki J.

Wiadomości Parazytologiczne

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2005

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tom 51

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nr 3

6

Effect of reduced glutathione [GSH] on activity of lysosomal system in subcellular fractions of mouse kidney

86%

Sliwa-Jozwik A., Jozwik A., Fronczyk W., Guszkiewicz A., Kolataj A.

Animal Science Papers and Reports

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2004

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tom 22

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nr 2

Investigated was the effect of intraperitoneal injections (twice daily for seven consecutive days) of reduced glutathione (GSH) on activity of selected lysosomal hydrolases (alanine aminopeptidase, leucine aminopeptidase, cathepsins D and L, acid phosphatase and N-acetyl-β-D-glucosaminidase) in lysosomal, microsomal and cytosol fractions of mouse kidney. Most notable effect of exogenous GSH on the activity of the enzymes considered was observed in lysosomal, while the least in microsomal fraction. After a series of GSH injections the activity of enzymes increased significantly mainly In lysosomal fraction. It is assumed that the increase in activity of lysosomal enzymes following the GSH injections results from a physiological response of renal cells to this antioxidant.

7

Hint2, the mitochondrial nucleoside 5’-phosphoramidate hydrolase; properties of the homogeneous protein from sheep (Ovis aries) liver

72%

Bretes E., Wojdyla-Mamon A. M., Kowalska J., Jamielity J., Kaczmarek R., Baraniak J., Guranowski A.

Acta Biochimica Polonica

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2013

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tom 60

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nr 2

Adenosine 5'-phosphoramidate (NH2-pA) is a rare natural nucleotide and its biochemistry and biological functions are poorly recognized. All organisms have proteins that may be involved in the catabolism of NH2-pA. They are members of the HIT protein family and catalyze hydrolytic splitting of NH2-pA to 5'-AMP and ammonia. At least five HIT proteins have been identified in mammals; however, the enzymatic and molecular properties of only Fhit and Hint1 have been comprehensively studied. Our study focuses on the Hint2 protein purified by a simple procedure to homogeneity from sheep liver mitochondrial fraction (OaHint2). Hint1 protein was also prepared from sheep liver (OaHint1) and the molecular and kinetic properties of the two proteins compared. Both function as homodimers and behave as nucleoside 5'-phosphoramidate hydrolases. The molecular mass of the OaHint2 monomer is 16 kDa and that of the OaHint1 monomer 14.9 kDa. Among potential substrates studied, NH2-pA appeared to be the best; the Km and kcat values estimated for this compound are 6.6 µM and 68.3 s-1, and 1.5 µM and 11.0 s-1 per natively functioning dimer of OaHint2 and OaHint1, respectively. Studies of the rates of hydrolysis of different NH2-pA derivatives show that Hint2 is more specific towards compounds with a P-N bond than Hint1. The thermostability of these two proteins is also compared.

8

Activity of selected hydrolases in ontogeny of drone Apis mellifera carnica

72%

Zoltowska K., Lipinski Z., Farjan M.

Journal of Apicultural Science

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2007

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tom 51

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nr 1

9

Moulds lytic enzymes in the production of Yarrowia high-protein feed additives

72%

Piegza M., Jurkiewicz M., Kancelista A., Laba W., Witkowska D.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

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2015

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tom 18

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nr 4

10

Effect of aluminum on the activity of model lysosomal hydrolases and the ultrastructure of mouse hepatocyte

72%

Krol T., Lach H., Wieczorek A., Lysek-Gladysinska M., Staszczyk K., Mycinska M., Gren A.

Acta Biologica Cracoviensia. Series Zoologia

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2004

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tom 46

11

Localization of oxido-reductases and hydrolases during adventitious root primordium initiation and emergence in hypocotyl cuttings of Phaseolus vulgaris L.

72%

Kakkar R. K., Rai V. K.

Acta Physiologiae Plantarum

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1994

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tom 16

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nr 4

12

The level of protein and activity of hydrolases after infection of honeybee larvae with entomopathogenic nematodes

72%

Zoltowska K., Lipinski Z., Lopienska E.

Journal of Apicultural Science

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2003

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tom 47

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nr 2

13

Activity of hydrolases in the reproductive system and in consecutive stages of Ascaris suum development

72%

Dziekonska-Rynko J.

Wiadomości Parazytologiczne

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2004

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tom 50

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nr 2

14

Assessment of MMP-2 and MMP-9 expression in LPS stimulated LS180 cell line

72%

Kubiatowski T., Korzeniowska-Olszanska J., Rozynska K., Filip A., Marzec B., Gawlowicz M., Koczkodaj D., Wojcierowski J.

Bulletin of the Veterinary Institute in Puławy. Supplement

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2002

15

The activity of selected hydrolases in excretion-secretion products and extracts from larvae and mature specimens of Cystidicola farionis

72%

Dziekonska-Rynko J., Rokicki J., Jablonowski Z.

Oceanological and Hydrobiological Studies

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2003

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tom 32

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nr 4

16

Some hydrolases and their substrates occuring in initial period of germination in Triticale grains of various pre-harvest sprouting resistance

72%

Niziolek S., Bartoszewicz K., Kaczkowski J.

Acta Physiologiae Plantarum

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1994

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tom 16

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nr 3

17

Wplyw aktywnosci wybranych hydrolaz na wartosc wypiekowa mak pszennych z ziarna porosnietego

58%

Dojczew D., Pietrych A., Haber T.

Żywność Nauka Technologia Jakość

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2003

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tom 10

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nr 3

93-100

Celem pracy było zbadanie wpływu porostu ziarna na zmiany aktywności enzymów amylolitycznych i proteolitycznych oraz określenie możliwości wykorzystania mąki z ziarna porośniętego w piekarstwie, po zastosowaniu witaminy C i glutenu witalnego jako polepszaczy. W próbach mąk wzorcowych i z ziarna porośniętego oznaczono: liczbę opadania, poziom azotu niebiałkowego, ogólną aktywność amylaz i proteaz oraz przeprowadzono analizę farinograficzną. Badania zakończono próbnym wypiekiem laboratoryjnym. Aktywność enzymów hydrolitycznych wzrosła w każdej próbie mąki z ziarna porośniętego. W mące z ziarna odmiany Korweta aktywność amylaz zwiększyła się o 80%, a z odmian Alba i Sakwa odpowiednio o 42 i 31%. Poziom aktywności proteaz również wzrósł znacząco, największy przyrost o 78% odnotowano w mące z ziarna odmiany Korweta. Stwierdzono zależność pomiędzy cechami fizycznymi ciasta i wielkością aktywności hydrolaz. Próba mąki z porośniętego ziarna Korweta charakteryzowała się najwyższym przyrostem aktywności amylaz i proteaz, jednocześnie jej parametry farinograficzne w największym stopniu uległy pogorszeniu. Odnotowano korzystny wpływ preparatów polepszających na pieczywo uzyskane z mąki z ziarna porośniętego.

18

Geotrichum hydrolytic activity in milled malt and barley medium

58%

Piegza M., Witkowska D., Stempniewicz R., Rywinska A.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

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2005

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tom 08

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nr 1

19

Arabidopsis thaliana Nudix hydrolase AtNUDT7 forms complexes with the regulatory RACK1A protein and gamma subunits of the signal transducing heterotrimeric G protein

58%

Olejnik K., Bucholc M., Anielska-Mazur A., Lipko A., Kujawa M., Modzelan M., Augustyn A., Kraszewska E.

Acta Biochimica Polonica

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2011

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tom 58

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nr 4

Arabidopsis thaliana AtNUDT7 Nudix pyrophosphatase hydrolyzes NADH and ADP-ribose in vitro and is an important factor in the cellular response to diverse biotic and abiotic stresses. Several studies have shown that loss-of-function Atnudt7 mutant plants display many profound phenotypes. However the molecular mechanism of AtNUDT7 function remains elusive. To gain a better understanding of this hydrolase cellular role, proteins interacting with AtNUDT7 were identified. Using AtNUDT7 as a bait in an in vitro binding assay of proteins derived from cultured Arabidopsis cell extracts we identified the regulatory protein RACK1A as an AtNUDT7-interactor. RACK1A-AtNUDT7 interaction was confirmed in a yeast two-hybrid assay and in a pull-down assay and in Bimolecular Fluorescence Complementation (BiFC) analysis of the proteins transiently expressed in Arabidopsis protoplasts. However, no influence of RACK1A on AtNUDT7 hydrolase catalytic activity was observed. In vitro interaction between RACK1A and the AGG1 and AGG2 gamma subunits of the signal transducing heterotrimeric G protein was also detected and confirmed in BiFC assays. Moreover, association between AtNUDT7 and both AGG1 and AGG2 subunits was observed in Arabidopsis protoplasts, although binding of these proteins could not be detected in vitro. Based on the observed interactions we conclude that the AtNUDT7 Nudix hydrolase forms complexes in vitro and in vivo with regulatory proteins involved in signal transduction. Moreover, we provide the initial evidence that both signal transducing gamma subunits bind the regulatory RACK1A protein.

20

Studies on the production of Phaseolus vulgaris tempe in discontinuously rotating bioreactor

58%

Miszkiewicz H., Bizukojc M., Rozwandowicz A., Bielecki S.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

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2005

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tom 08

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nr 2

Ograniczanie wyników

The activity of hydrolases of larval stages of Anisakis simplex [Nematoda]

Geotrichum candidum Link 1809: hydrolases activity and own methoof digestive tract strains biotyping

Effect of avocado oil on the induction of yeast hydrolases

The activity of hydrolases of entomopathogenic nematodes

Activity of selected hydrolases in excretion-secretion products and extracts od adult Contracaecum rudolphii

Effect of reduced glutathione [GSH] on activity of lysosomal system in subcellular fractions of mouse kidney

Hint2, the mitochondrial nucleoside 5’-phosphoramidate hydrolase; properties of the homogeneous protein from sheep (Ovis aries) liver

Activity of selected hydrolases in ontogeny of drone Apis mellifera carnica

Moulds lytic enzymes in the production of Yarrowia high-protein feed additives

Effect of aluminum on the activity of model lysosomal hydrolases and the ultrastructure of mouse hepatocyte

Localization of oxido-reductases and hydrolases during adventitious root primordium initiation and emergence in hypocotyl cuttings of Phaseolus vulgaris L.

The level of protein and activity of hydrolases after infection of honeybee larvae with entomopathogenic nematodes

Activity of hydrolases in the reproductive system and in consecutive stages of Ascaris suum development

Assessment of MMP-2 and MMP-9 expression in LPS stimulated LS180 cell line

The activity of selected hydrolases in excretion-secretion products and extracts from larvae and mature specimens of Cystidicola farionis

Some hydrolases and their substrates occuring in initial period of germination in Triticale grains of various pre-harvest sprouting resistance

Wplyw aktywnosci wybranych hydrolaz na wartosc wypiekowa mak pszennych z ziarna porosnietego

Geotrichum hydrolytic activity in milled malt and barley medium

Arabidopsis thaliana Nudix hydrolase AtNUDT7 forms complexes with the regulatory RACK1A protein and gamma subunits of the signal transducing heterotrimeric G protein

Studies on the production of Phaseolus vulgaris tempe in discontinuously rotating bioreactor