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Characteristics of the regional human milk bank in Poland - donors, recipients and nutritional value of human milk

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Barbarska O., Zielinska M., Pawlus B., Wesolowska A.
Roczniki Państwowego Zakładu Higieny
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2017
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tom 68
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nr 4
Background. In case of shortage of breast milk despite proper lactation care or the poor state of the mother’s health, breast milk from human milk bank is recommended for feeding preterm infants. Objective. This study retrospectively evaluated the first year of the operation of the Regional Human Milk Bank. Material and methods. Data concerning donors was collected in the human milk bank during the cooperation. The clinical characteristics of the recipients was made on the basis of medical documentation from the Holy Family Hospital in Warsaw, Poland. Analysis of nutritional value was performed with the human milk analyzer (MIRIS AB). Results. In the first year of activity, 45 voluntary donors established cooperation, donating from 650 to 32030 ml of human milk. The content of nutrients in milk provided by donors was variable - protein 0.4-1.5 g / 100 ml, fat 1.1-7.4 g / 100 ml, carbohydrates 6.3-7.9 g / 100 ml. The average length of using donated human milk was 4 days and the average volume of milk for one infant was 282 ml. Conclusions. The donor profiles have a significant impact on the milk composition form HMB. The nutritional value can be improved by recruitment donors from mothers that gave birth prematurely and by beginning donation at earlier stages of lactation as soon as lactation is stabilized. In case of shortage of mothers own milk the immediate implementation of donors milk as a short-term support can significantly reduce the food intolerance incidence in the group of prematurely born infants
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A comparison of ABTS and DPPH methods for assessing the total antioxidant capacity of human milk

100%
Martysiak-Zurowska D., Wenta W.
Acta Scientiarum Polonorum. Technologia Alimentaria
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2012
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tom 11
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nr 1
Introduction. The Total Antioxidant Capacity (TAC) of human milk reflects the concentration and the activity of many components which prevent oxidative degradation of fats and proteins. This study compares the effectiveness of ABTS and DPPH tests with regard to the recovery, precision and sensitivity (detection and quantification limit) of (TAC) values in human milk. Material and methods. TAC values were determined in twenty five samples of human milk obtained from healthy mothers, residents of Gdańsk, on the 14,h day postpartum. Results. The average TAC of human milk determined by ABTS assay was 19.61 ±3.311 mg TE (Trolox Equivalents)/100 cm3, the average values obtained by the DPPH assay reached 9.95 ±4.36 mg TE/100 cm3. For each milk sample the TAC determined by the ABTS test was significantly higher than the values produced by the DPPH test. The above findings can be attributed to the presence of substances whose spectra overlap with DPPH’ spectra. ABTS test was characterised by a higher sensitivity and repeatability of the determination of TAC in human milk compared to the DPPH test. Conclusions. Comparing the calculated values for the validation parameters of both methods and taking into account the solubility of DPPH only in polar matrices, slower reaction of selected antioxidants with DPPH radical, and the presence in human milk constituents absorbing electromagnetic radiation in the absorption of DPPH be assumed that the ABTS test is more appropriate method of determining of TAC in breast milk.
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