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2-methyl-thiazolidine-2,4-dicarboxylic acid protects against paracetamol induced toxicity in human liver derived HepG2 cells

100%

Wlodek L., Rommelspacher H.

Acta Biochimica Polonica

1997

tom 44

nr 4

The effects of 2-methyl-thiazolidine-2,4-dicarboxylic acid (CP) on paracetamol-induced toxicity were investigated and evaluated in a human liver derived HepG2 cell line. Incubation of the cells with CP (2 mM and 10 mM) drastically attenuated the GSH and cysteine depletion caused by toxic concentrations of paracetamol (1 mM and 5 mM). When CP (10 mM) was introduced alone into the medium, the level of malondialdehyde and the reactive oxygen species were maintained at the control levels with a simultaneous increase of non-protein sulfhydryl in the cells. Thus, the results of our work prove that CP is a non-toxic precursor of cysteine and GSH, and successfully prevents paracetamol toxicity in HepG2 cells.

Determination of vanadium in liver

80%

Pacer-Strach M., Czarnowski W.

Acta Toxicologica

2003

tom 11

nr 1

Molecular cloning and expression analysis of a new gene for short-chain dehydrogenase-reductase 9

80%

Liu S., Huang C., Li D., Ren W., Zhang H., Qi M., Li X., Yu L.

Acta Biochimica Polonica

2007

tom 54

nr 1

We report here the cloning and characterization of a novel human short-chain dehydrogenases/ reductase gene SCDR9, isolated from a human liver cDNA library, and mapped to 4q22.1 by browsing the UCSC genomic database. SCDR9 containing an ORF with a length of 900 bp, encoding a protein with a signal peptide sequence and an adh_short domain. GFP localization shows SCDR9 protein concentrated in some site of the cytoplasm, but not in the ER. Expression pattern in eighteen tissues revealed that SCDR9 is expressed highly in liver. Soluble recombinant protein was successfully purified from Escherichia coli using pET28A(+) expression vector. Our data provides important information for further study of the function of the SCDR9 gene and its products.

Ograniczanie wyników

2 Acta Biochimica Polonica

1 Acta Toxicologica

1 Czarnowski W.

1 Huang C.

1 Li D.

1 Li X.

1 Liu S.

1 Pacer-Strach M.

1 Qi M.

1 Ren W.

1 Rommelspacher H.

1 Wlodek L.

1 Yu L.

1 Zhang H.

1 2007

1 2003

1 1997

Wyniki wyszukiwania

2-methyl-thiazolidine-2,4-dicarboxylic acid protects against paracetamol induced toxicity in human liver derived HepG2 cells

Determination of vanadium in liver

Molecular cloning and expression analysis of a new gene for short-chain dehydrogenase-reductase 9