Wyniki wyszukiwania

1

P53 and P73 expression in ovarian neoplasms

100%

Wasikiewicz D., Curylo D., Lindner K., Luszczyn A., Pyszel A.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

2

The role of copper in tumor angiogenesis

88%

Nasulewicz A., Wietrzyk J., Opolski A.

Cellular and Molecular Biology Letters

|

2002

|

tom 07

|

nr Suppl.

3

TP53 and mutations in human cancer

88%

Szymanska K., Hainaut P.

Acta Biochimica Polonica

|

2003

|

tom 50

|

nr 1

TP53 is the most frequently mutated gene in human cancer, with a predominance of missense mutations scattered over 200 codons. In many cancers, specific mutation patterns can be identified, which are shaped by site-specific mutagenesis and by biological selection. In tobacco-related cancers (lung, head and neck), organ-specific patterns are observed, with many mutations compatible with the ones experimentally induced by tobacco carcinogens. In several other cancers, such as squamous cell carcinoma of the oesophagus or hepatocellular carcinoma (HCC), mutation patterns show geographic variations between regions of high and low incidence, suggesting a role for region-specific risk factors. HCC from high-incidence regions showing also a high prevalence of a specific Ser-249 TP53 mutation is one of the most striking examples of a mutagen fingerprint. All such assessments are useful to generate clues on the mutagenic mechanisms involved in human cancer. Moreover, it has been shown that DNA retrieved from plasma can be successfully used for detection of TP53 mutations, which gives hope for earlier more accurate detection of human cancers.

4

Immunospecific nuclear proteins of colorectal tumors

75%

Szymczyk P., Krajewska W. M., Jakubik J., Berner A., Berner J., Brocki M., Rajczyk B., Kilianska Z.

Cellular and Molecular Biology Letters

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1996

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tom 01

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nr 4

Electrophoretically specific nuclear proteins of human colon adenocarcinoma with mol. wt of 35-40 kDa were used as immunogen to produce rabbit antiserum. Expression of cancer-specific antigens was investigated by Western blot technique among nuclear proteins from normal and cancerous mucosa. Obtained antiserum crossreacted mainly with 36 kDa polypeptide in 23 of 26 (88,5%) colorectal tumor nuclear fractions but not with any of normal ones. It was also observed that this antiserum recognized 36 kDa antigen in 10 of 12 and 6 of 7 nuclear fractions from other cancers, ie. gastric and lung, respectively. In part of studied tumors antiserum crossreacted also with the antigens of 38 and 32-33 kDa. Expression of 36 and 32-33 kDa components seems to be correlated with colorectal cancer progression from A to B stage of disease according to the classification of Dukes. Immunoblot analysis revealed that cancer-specific 36 kDa polypeptide, mainly associated with nuclear compartment, can be also detected within 10P and 100P fractions of colorectal tumors.

5

A novel non-catalytic inhibitory mechanism employed by the CSK-homologous kinase [CHK] to inactivate SRC-family protein kinases

75%

Cheng H. C., Chong Y. P., Mulhern T.

Cellular and Molecular Biology Letters

|

2005

|

tom 10

|

nr Suppl.2

6

Mutations in the KRAS gene in ovarian tumors

63%

Dobrzycka B., Terlikowski S. J., Kowalczuk O., Niklinska W., Chyczewski L., Kulikowski M.

Folia Histochemica et Cytobiologica

|

2009

|

tom 47

|

nr 2

221-224

7

Vitamin C inhibits random migration of malignant pleural effusion mononuclear cells

63%

Prokic L., Vilic I., Vuckovic-Dekic L., Neskovic-Konstantinovic Z., Susnjar S.

Archivum Immunologiae et Therapiae Experimentalis

|

1997

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tom 45

|

nr 1

8

The effect of Topotecan on oxidative stress in MCF-7 human breast cancer cell line

63%

Timur M., Akbas S. H., Ozben T.

Acta Biochimica Polonica

|

2005

|

tom 52

|

nr 4

Purpose. Topotecan, a semisynthetic water-soluble derivative of camptothecin exerts its cytotoxic effect by inhibiting topoisomerase I and causes double-strand DNA breaks which inhibit DNA function and ultimately lead to cell death. In previous studies it was shown that camptothecin causes ROS formation. The aim of this study was to investigate if Topotecan like camptotecin causes oxidative stress in MCF-7 human breast cancer cell line. Determining the oxidant effect of Topotecan may elucidate a possible alternative mechanism for its cytotoxicity. Experimental design. MCF-7 cells were cultured and exposed to Topotecan for 24 h at 37°C. The viability of the cells (% of control) was measured using the colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Lipid peroxidation (TBARS), protein oxidation (carbonyl content), sulfhydryl, glutathione (GSH) levels, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities were determined in MCF-7 cells with and without Topotecan incubation. Results. We found the IC50 concentration of Topotecan as 0.218 μM in MCF-7 cells. This concentration of Topotecan was used in the incubations of the cells. Our data indicated increased oxidative status, as revealed by increased lipid peroxidation and protein oxidation, and decreased GSH and sulfhydryl levels in MCF-7 cells exposed to Topotecan compared to control cells. In contrast, there was a slight increase in SOD and a significant increase in GPx and catalase activity in MCF-7 cells incubated with Topotecan compared to the control. Conclusions. These results support our hypothesis that Topotecan increases oxidative stress in MCF-7 cells.

9

Novel Ru[III], Rh[III], Pd[II] and Pt[II] complexes with ligands incorporating azole and pyrimidine rings. I. Antiproliferative activity in vitro

63%

Wisniewski M. Z., Wietrzyk J., Opolski A.

Archivum Immunologiae et Therapiae Experimentalis

|

2000

|

tom 48

|

nr 1

10

STAT3, HIF-1alpha, EPO and EPOR - signaling proteins in human primary ductal breast cancers

63%

Wincewicz A., Sulkowska M., Koda M., Lesniewicz T., Kanczuga-Koda L., Sulkowski S.

Folia Histochemica et Cytobiologica

|

2007

|

tom 45

|

nr 2

11

ERK is involved in the differentiation induced by diallyl disulfide in the human gastric cancer cell line MGC803

63%

Ling H., Zhang L., Su Q., Song Y., Luo Z., Zhou X. T., Zeng X., He J., Tan H., Yuan J.

Cellular and Molecular Biology Letters

|

2006

|

tom 11

|

nr 3

Diallyl disulfide (DADS) is a major constituent of garlic. Previously, we found that DADS both inhibited proliferation in human gastric cancer cells in vitro and in vivo, and induced G2/M arrest. In this study, we investigated whether this differentiation effect was induced by DADS in human gastric cancer MGC803 cells, and whether it was related to an alteration in ERK activity. The results showed that the growth of MGC803 cells was inhibited by DADS. Cells treated with DADS displayed a lower nucleocytoplasmic ratio and tended to form gland and intercellular conjunction structures. The ConA-mediated cell agglutination ratio and cells’ ALP specific activity decreased. In MGC803 cells, dye transfer was limited to a few cells neighbouring the dye-injected cell and to a depth of 1–2 layers beneath the scrape site. However, after treatment with DADS, the LY (Lucifer Yellow) was transferred to several cells immediately neighbouring the microinjected cell and to a depth of 2–4 cell layers from the scrape site. This indicated that DADS induced differentiation in MGC803 cells. Western blot analysis revealed that although DADS did not influence the quantity of ERK1/2 protein expressed, it did decrease its phosphorylation in a concentration-dependent manner, compared with the controls. At 30 mg·L−1, DADS inhibited the activation of ERK1/2 in 15–30 min. These results suggested that the DADS-induced differentiation of MGC803 cells involved an alteration of the ERK1/2 signaling pathway.

12

The cell type-specific effect of TAp73 isoforms on the cell cycle and apoptosis

51%

Holcakova J., Ceskova P., Hrstka R., Muller P., Dubska L., Coates P. J., Palecek E., Vojtesek B.

Cellular and Molecular Biology Letters

|

2008

|

tom 13

|

nr 3

404-420

p73, a member of the p53 family, exhibits activities similar to those of p53, including the ability to induce growth arrest and apoptosis. p73 influences chemotherapeutic responses in human cancer patients, in association with p53. Alternative splicing of the TP73 gene produces many p73 C- and N-terminal isoforms, which vary in their transcriptional activity towards p53-responsive promoters. In this paper, we show that the C-terminal spliced isoforms of the p73 protein differ in their DNA-binding capacity, but this is not an accurate predictor of transcriptional activity. In different p53-null cell lines, p73β induces either mitochondrial-associated or death receptor-mediated apoptosis, and these differences are reflected in different gene expression profiles. In addition, p73 induces cell cycle arrest and p21WAF1 expression in H1299 cells, but not in Saos-2. This data shows that TAp73 isoforms act differently depending on the tumour cell background, and have important implications for p73-mediated therapeutic responses in individual human cancer patients.

13

Quantitative analysis of the level of p53 and p21 WAF1 mRNA in human colon cancer HT-29 cells treated with inositol hexaphosphate

51%

Weglarz L., Molin I., Orchel A., Parfiniewicz B., Dzierzewicz Z.

Acta Biochimica Polonica

|

2006

|

tom 53

|

nr 2

The aim of this study was to analyze the molecular mechanism of inositol hexaphosphate (InsP6) action through which it may inhibit proliferation of colon cancer cells and cell cycle progression. A kinetic study of p53 and p21WAF1 mRNA increase was performed on human colon cancer HT-29 cells after treatment with 1, 5 and 10 mM InsP6 for 6, 12, 24 and 48 h. Real-time-QPCR based on TaqMan methodology was applied to analyze quantitatively the transcript levels of these genes. The transcription of β-actin and GAPDH genes was assessed in parallel to select the control gene with least variability. The 2-ΔΔCt method was used to analyze the relative changes in gene transcription. InsP6 stimulated p53 and p21WAF1 expression at the mRNA level, with the highest increase in p21WAF1 mRNA occurring at 24 h, i.e., following the highest increase in p53 mRNA observed at 12 h. Based on these studies it may be concluded that the ability of InsP6 to arrest the cell cycle may be mediated by the transcriptional up-regulation of the p53-responsive p21WAF1 gene.

14

The ability of new formamidine sugar-modified derivatives of daunorubicin to stimulate free radical formation in three enzymatic systems: NADH dehydrogenase, NADPH cytochrome p450 reductase and xanthine oxidase

51%

Pawlowska J., Tarasiuk J., Borowski E., Wasowska M., Oszczapowicz I., Wolf C. R.

Acta Biochimica Polonica

|

2000

|

tom 47

|

nr 1

Some sterically hindered N-substituted derivatives of daunorubicin are known to be poor substrates for NADH dehydrogenase, NADPH cytochrome P450 reductase and xanthine oxidase. In consequence, poor oxygen radical generation by these compounds is observed. In this study we examined a new family of sugar-N-substituted derivatives of daunorubicin bearing a bulky substituent introduced on the nitrogen atom through the amidine spacer. These compounds were found to be very active in radical formation catalyzed by all three studied enzymes. Thus, the introduction of a heterocyclic ring, even if it is bulky but flexible, on the nitrogen atom of daunosamine moiety through the one-atom spacer (amidine group), does not induce the steric hindrance effect on the interaction of daunorubicin derivatives with these flavoprotein enzymes.

15

EBV - infected LCL and B-celL exhibit different expression pattern of p27 and Rb genes family

51%

Marzec B., Kubiatowski T., Gawlowicz M., Szczesniak D., Filip A., Koczkodaj D., Nesina I., Wojcierowski J.

Bulletin of the Veterinary Institute in Puławy. Supplement

|

2002

16

Mitochondrial DNA mutations in human neoplasia

51%

Czarnecka A. M., Golik P., Bartnik E.

Journal of Applied Genetics

|

2006

|

tom 47

|

nr 1

Many models of tumour formation have been put forth so far. In general they involve mutations in at least three elements within the cell: oncogenes, tumour suppressors and regulators of telomere replication. Recently numerous mutations in mitochondria have been found in many tumours, whereas they were absent in normal tissues from the same individual. The presence of mutations, of course, does not prove that they play a causative role in development of neoplastic lesions and progression; however, the key role played by mitochondria in both apoptosis and generation of DNA-damaging reactive oxygen species might indicate that the observed mutations contribute to tumour development. Recent experiments with nude mice have proven that mtDNA mutations are indeed responsible for tumour growth and exacerbated ROS production. This review describes mtDNA mutations in main types of human neoplasia.

17

Evaluation of apoptotic activity of new condensed pyrazole derivatives

45%

Toton E., Ignatowicz E., Bernard M. K., Kujawski J., Rybczynska M.

Journal of Physiology and Pharmacology

|

2013

|

tom 64

|

nr 1

Ograniczanie wyników

P53 and P73 expression in ovarian neoplasms

The role of copper in tumor angiogenesis

TP53 and mutations in human cancer

Immunospecific nuclear proteins of colorectal tumors

A novel non-catalytic inhibitory mechanism employed by the CSK-homologous kinase [CHK] to inactivate SRC-family protein kinases

Mutations in the KRAS gene in ovarian tumors

Vitamin C inhibits random migration of malignant pleural effusion mononuclear cells

The effect of Topotecan on oxidative stress in MCF-7 human breast cancer cell line

Novel Ru[III], Rh[III], Pd[II] and Pt[II] complexes with ligands incorporating azole and pyrimidine rings. I. Antiproliferative activity in vitro

STAT3, HIF-1alpha, EPO and EPOR - signaling proteins in human primary ductal breast cancers

ERK is involved in the differentiation induced by diallyl disulfide in the human gastric cancer cell line MGC803

The cell type-specific effect of TAp73 isoforms on the cell cycle and apoptosis

Quantitative analysis of the level of p53 and p21 WAF1 mRNA in human colon cancer HT-29 cells treated with inositol hexaphosphate

The ability of new formamidine sugar-modified derivatives of daunorubicin to stimulate free radical formation in three enzymatic systems: NADH dehydrogenase, NADPH cytochrome p450 reductase and xanthine oxidase

EBV - infected LCL and B-celL exhibit different expression pattern of p27 and Rb genes family

Mitochondrial DNA mutations in human neoplasia

Evaluation of apoptotic activity of new condensed pyrazole derivatives