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The objective of the study was to determine if there were differences in Cr, Mn, Cu, Cd, Pb and Ni levels in coat hair and hoof horn between Polish Konik horses maintained in stabled and reserve systems in Roztocze National Park in Poland. Summer and winter feeding seasons (seasons of the year in case of hooves) and age of horses were taken into account. 107 samples of hair and hooves, as well as all kinds of feed and sources of water available for the horses were examined. The results of the study indicate that the concentration of the elements in the coat hair, hoof horn, feeds and water is usually too low to show possible influence of the system of maintenance in feeding seasons and age effect. The reserve horses are not more exposed to heavy metal contamination than the stabled horses. In the hair, solely Mn level is affected by the systems of maintenance in summer and winter feeding seasons: during winter it is higher, particularly in the stabled system, than during summer. In hooves, Cr concentration is higher in stabled horses than in reserve horses and conversely, Cu content is higher in the reserve horses. Mn content is age-affected both in hair and hooves, likewise is Cu content in the hooves. The results indicate a necessity of Cu compound supplementation in feeding the horses in both systems of maintenance.
Sexual dimorphism in the skull of Spanish ibex Capra pyrenaica Schinz, 1838 is analyzed by means of univariate and multivariate techniques in one of the most representative populations of the species, located in the Sierra de Gredos, central Spain. Thirty eight measurements were used for the analysis: 30 of the skull and mandible, and 8 of the horn. Eighty three skulls (40 males and 43 females) of individuals older than 4 years were used. In 36 out of the 38 variables males were significantly larger than females. Horn characters showed the highest variability and also the most significant differences between sexes. Values of Mahalanobis distance between males and females were high (D2 = 323.1). When Factor Analysis is performed with the whole set of 38 variables, 7 Principal Factors were extracted, scores of Principal Factor 2 (related with horn variables) and Principal Factor 3 {related to molar toothrow length) showing significant differences between sexes. In this analysis, horn characters (Principal Factor 2) accumulate the sexual dimorphism of other skull traits. However, if the same analysis is performed without horn characters, 5 Principal Factors were extracted, scores of three Principal Factors showing significant differ­ences between sexes. Highest sexual dimorphism in this analysis corresponds to the Principal Factor 3 (molar toothrow), follow the Principal Factor 2 (posterior region of the skull) and Principal Factor 1 (main length measurements). This suggest that the high degree of sexual dimorphism observed in the Spanish ibex skull is mainly related to horn traits and, to a lesser extent, of other skull features like molar toothrow length.
To investigate phylogenetic relationships among 9 genera of the Caprinae (Capra, Ammotragus, Hemitragus, Pseudois, Ovis, Rupicapra, Oreamnos, Nemorhaedus, Capri- cornis) behaviours involved in courtship and mating, aggression, threat, dominance, submission, and marking of adult males were subjected to phylogenetic analysis. Based on all 32 characters and 96 character states investigated, phylogenetic patterns generally were in good agreement with biochemical-genetic data avaliable. Discordance between phylogenetic trees constructed from behavioural and from biochemical-genetic traits as to the position of Ammotragus turned out to be associated with ethological functional categories. Behaviours involved in courtship and mating were identified as the most reliable ones for phylogenetic studies. Courtship displays function as isolation mechanisms among closely related taxa. This is of paramount importance in those forms where secondary sexual characters such as horns are poorly differentiated while in highly evolved taxa size and shape of horns may also trigger readiness for mating in estrous females.
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