Wyniki wyszukiwania

1

Differences in responses upon corticosteroid therapy between smoking and non-smoking patients with COPD

100%

Van Overveld F. J., Demkow U., Gorecka D., De Backer W. A., Zielinski J.

Journal of Physiology and Pharmacology. Supplement

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2006

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tom 57

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nr 4

273-282

Inhaled corticosteroids have a high level of topical anti-inflammatory activity. However, in patients with COPD these drugs have been reported to exert limited effects. A reduction in histone deacetylase (HDAC) activity is suggested to prevent the anti-inflammatory action of corticosteroids. Cigarette smoke is known to reduce HDAC expression. The aim of this study is to compare the outcome of corticosteroid therapy in both smoking and non-smoking COPD patients. Twenty-three smoking patients and 18 ex-smoking patients with COPD were treated with inhaled corticosteroids for a period of 2 months. Blood and induced sputum samples were collected before and after treatment. Values of FEV1 %-predicted did not change upon the therapy, but there was a trend to improve in the ex-smokers (63.1 64.8%-pred.), compared with a decrease in the smokers (63.3 61.6%-pred.). The levels of the pro-inflammatory cytokine IL-8 increased in the group of smokers from 379 ±78 to 526 ±118 ng/ml. Although not significant, a slight decrease from 382 ±70 to 342 ±62 ng/ml was observed in the group of ex-smokers. The neutrophil related elastase activity showed similar effects after steroid treatment, it went up from 36.4 ±12.0 to 113.5 ±9.7 nmol/l in smokers, and decreased from 346.2 ±72.1 to 131.1 ±6.5 nmol/l in ex-smokers with COPD. These results support the evidence that inhaled corticosteroids have no anti-inflammatory effects in COPD patients, but only when these patients are still smoking. Smoking cessation seems the best therapy for COPD patients.

2

Natural agents-mediated targeting of histone deacetylases

100%

Farooqi A. A., Kamran-ul-Hassan Naqvi S., Perk A. A., Yanar O., Tabassum S., Ahmad M. S., Mansoor Q., Ashry M. S., Ismail M., Naoum G. E., Arafat W. O.

Archivum Immunologiae et Therapiae Experimentalis

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2018

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tom 66

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nr 1

3

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Impact of 3-hydroxybutyrate on the expression of plant histone deacetylases, methylases and crucial genes of the phenylpropanoid pathway

100%

Mierziak J., Dzialo M., Kulma A., Szopa-Skorkowski J.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

4

Effect of trichostatin a on transcription of selected signal molecule genes in Jurkat T cells

100%

Januchowski R., Jagodzinski P. P.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr Suppl.2

5

Molecular characterisation of retinoblastoma protein interactions with the nuclear lamins

100%

Markiewicz E., Venables R., Quinlan R., Hutchison C. J.

Cellular and Molecular Biology Letters

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2000

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tom 05

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nr 2

6

Nuclear receptors, their coactivators and modulation of transcription

100%

Manteuffel-Cymborowska M.

Acta Biochimica Polonica

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1999

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tom 46

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nr 1

Nuclear receptors arc ligand-dependent transcription factors which can also be activated in the absence of their lipophilic ligands by signaling substances acting on cell membrane receptors. This ligand-independent activation indicates the importance of nuclear receptor phosphorylation for their function. Nuclear receptor- mediated transcription of target genes is further increased by interactions with recruited coactivators forming a novel family of nuclear proteins. CBP/p300, a coactivator of different classes of transcription factors, including the tumor suppressor protein p53, plays a special role acting as a bridging protein between inducible transcription factors and the basal transcription apparatus, and as an integrator of diverse signaling pathways. Coactivators of nuclear receptors and associated proteins forming a multicomponent complex have an intrinsic histone acetylase activity in contrast to nuclear receptor and heterodimer Mad-Max corepressors, which recruit histone deacetylase. Similarly the Rb protein interacts with histone deacetylase to repress transcription of cell cycle regulatory genes. Targeted histone acetylation/dca- cetylation results in remodeling of chromatin structure and correlates with activation/repression of transcription. Recent data point to the important role of coactivator proteins associated with inducible transcription factors in transcription regulation, and in the integration of multiple signal transduction pathways within the nucleus.

7

Sirt7-dependent inhibition of cell growth and proliferation might be instrumental to mediate tissue integrity during aging

84%

Vakhrusheva O., Braeuer D., Liu Z., Braun T., Bober E.

Journal of Physiology and Pharmacology. Supplement

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2008

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tom 59

|

nr 9

201-212

8

Effect of trichostatin A on CD4 surface density in peripheral blood T cells

84%

Kozlowska A., Jagodzinski P. P.

Folia Histochemica et Cytobiologica

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2006

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tom 44

|

nr 4

9

WDR5, ASH2L, and RBBP5 control the efficiency of FOS transcript processing

67%

Teoh P. L., Sharrocks A. D.

Cellular and Molecular Biology Letters

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2014

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tom 19

|

nr 2

H3K4 trimethylation is strongly associated with active transcription. The deposition of this mark is catalyzed by SET-domain methyltransferases, which consist of a subcomplex containing WDR5, ASH2L, and RBBP5 (the WAR subcomplex); a catalytic SET-domain protein; and additional complexspecific subunits. The ERK MAPK pathway also plays an important role in gene regulation via phosphorylation of transcription factors, co-regulators, or histone modifier complexes. However, the potential interactions between these two pathways remain largely unexplored. We investigated their potential interplay in terms of the regulation of the immediate early gene (IEG) regulatory network. We found that depletion of components of the WAR subcomplex led to increased levels of unspliced transcripts of IEGs that did not necessarily reflect changes in their mature transcripts. This occurs in a manner independent from changes in the H3K4me3 levels at the promoter region. We focused on FOS and found that the depletion of WAR subcomplex components affected the efficiency of FOS transcript processing. Our findings show a new aspect of WAR subcomplex function in coordinating active transcription with efficient pre-mRNA processing.

10

Effects of the histone deacetylase inhibitor, trichostatin A, in a chronic allergic airways disease model in mice

67%

Royce S. G., Dang W., Yuan G., Tran J., El-Osta A., Karagiannis T. C., Tang M. L. K.

Archivum Immunologiae et Therapiae Experimentalis

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2012

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tom 60

|

nr 4

11

Antileukemic activity of combined epigenetic agents, DNMT inhibitors zebularine and RG108 with HDAC inhibitors, against promyelocytic leukemia HL-60 cells

67%

Savickiene J., Treigyte G., Borutinskaite V.-V., Navakauskiene R.

Cellular and Molecular Biology Letters

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2012

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tom 17

|

nr 4

DNMT inhibitors are promising new drugs for cancer therapies. In this study, we have observed the antileukemic action of two diverse DNMT inhibitors, the nucleoside agent zebularine and the non-nucleoside agent RG108, in human promyelocytic leukemia (PML) HL-60 cells. Zebularine but not RG108 caused dose- and time-dependent cell growth inhibition and induction of apoptosis. However, co-treatment with either drug at a non-toxic dose and all trans retinoic acid (RA) reinforced differentiation to granulocytes, while 24 or 48 h-pretreatment with zebularine or RG108 followed by RA alone or in the presence of HDAC inhibitors (sodium phenyl butyrate or BML-210) significantly accelerated and enhanced cell maturation to granulocytes. This occurs in parallel with the expression of a surface biomarker, CD11b, and early changes in histone H4 acetylation and histone H3K4me3 methylation. The application of both drugs to HL-60 cells in continuous or sequential fashion decreased DNMT1 expression, and induced E-cadherin promoter demethylation and reactivation at both the mRNA and the protein levels in association with the induction of granulocytic differentiation. The results confirmed the utility of zebularine and RG108 in combinations with RA and HDAC inhibitors to reinforce differentiation effects in promyelocytic leukemia.

12

Molecular basis of chronic inflammation in lung diseases: new therapeutic approach

67%

Mroz R. M., Noparlik J., Chyczewska E., Braszko J. J., Holownia A.

Journal of Physiology and Pharmacology. Supplement

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2007

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tom 58

|

nr 5

13

Sirtuin inhibition increases the rate of non-homologous end-joining of DNA double strands breaks

67%

Wojewodzka M., Kruszewski M., Buraczewska I., Xu W., Massuda E., Zhang J., Szumiel I.

Acta Biochimica Polonica

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2007

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tom 54

|

nr 1

Sirtuins (type III histone deacetylases) are an important member of a group of enzymes that modify chromatin conformation. We investigated the role of sirtuin inhibitor, GPI 19015, in double strand break (DSB) repair in CHO-K1 wt and xrs-6 mutant cells. The latter is defective in DNA-dependent protein kinase (DNA-PK)-mediated non-homologous end-joining (D-NHEJ). DSB were estimated by the neutral comet assay and histone γH2AX foci formation. We observed a weaker effect of GPI 19015 treatment on the repair kinetics in CHO wt cells than in xrs6. In the latter cells the increase in DNA repair rate was most pronounced in G1 phase and practically absent in S and G2 cell cycle phases. The decrease in the number of histone γH2AX foci was faster in xrs6 than in CHO-K1 cells. The altered repair rate did not affect survival of X-irradiated cells. Since in G1 xrs6 cells DNA-PK-dependent non-homologous end-joining, D-NHEJ, does not operate, these results indicate that inhibition of sirtuins modulates DNA-PK-independent (backup) non-homologous end-joining, B-NHEJ, to a greater extent than the other DSB repair system, D-NHEJ.

Ograniczanie wyników

Differences in responses upon corticosteroid therapy between smoking and non-smoking patients with COPD

Natural agents-mediated targeting of histone deacetylases

Impact of 3-hydroxybutyrate on the expression of plant histone deacetylases, methylases and crucial genes of the phenylpropanoid pathway

Effect of trichostatin a on transcription of selected signal molecule genes in Jurkat T cells

Molecular characterisation of retinoblastoma protein interactions with the nuclear lamins

Nuclear receptors, their coactivators and modulation of transcription

Sirt7-dependent inhibition of cell growth and proliferation might be instrumental to mediate tissue integrity during aging

Effect of trichostatin A on CD4 surface density in peripheral blood T cells

WDR5, ASH2L, and RBBP5 control the efficiency of FOS transcript processing

Effects of the histone deacetylase inhibitor, trichostatin A, in a chronic allergic airways disease model in mice

Antileukemic activity of combined epigenetic agents, DNMT inhibitors zebularine and RG108 with HDAC inhibitors, against promyelocytic leukemia HL-60 cells

Molecular basis of chronic inflammation in lung diseases: new therapeutic approach

Sirtuin inhibition increases the rate of non-homologous end-joining of DNA double strands breaks